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Regeneration of fertile plants from embryogenic suspension culture protoplasts of Sorghum vulgare

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Abstract

Protoplasts were isolated from immature inflorescence-derived embryogenic suspension cultures of two cultivars of Sorghum vulgare. The protoplasts were cultured in a modified K8P liquid medium. They started to divide after 4–5 days of culture, and achieved 16.8% division frequency by 10 days. Protocalli proliferated further upon transfer to C1 solid medium. After that, they were moved to C1 differentiation medium to induce shoot formation, followed by whole plant regeneration. So far, 60 plants have been obtained, with only two albinos. Some of these have been transplanted to soil in pots and grown to flowering and have set seeds.

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Abbreviations

BA:

6-benzylaminopurine

CH:

casein hydrolysate

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

3-indoleacetic acid

IBA:

3-indolebutyric acid

KT:

kinetin

PVP:

polyvinylpyrrolidone

References

  • Brar DS, Rambold S, Constabel F, Gamborg OL (1980) Z Pflanzenphysiol 96:269–275

    Google Scholar 

  • Brettell RIS, Wernicke W, Thomas E (1980) Protoplasma 104:141–148

    Google Scholar 

  • Chen YR, Wang P (1979) Hereditas (Beijing) 1:31–33

    Google Scholar 

  • Chourey PS, Sharpe DZ (1985) Plant Sic 39:171–175

    Google Scholar 

  • Dunstan DI, Short KC, Thomas E (1978) Ptotoplasma 97:251–260

    Google Scholar 

  • Dunstan DI, Short KC, Dhaliwal H, Thomas E (1979) Protoplasma 101:355–361

    Google Scholar 

  • Gamborg OL, Shyluk JP, Brar DS, Constabel F (1977) Plant Sic Lett 10:67–74

    Google Scholar 

  • Kao KN (1977) Mol Gen Genet 150:225–230

    Google Scholar 

  • Mu QH, Chen ZG, Yang ZT, Wang YX, Zhang ZM, Liu ZD, Zhang GL, Tian HX (1982) Kexue Tongbao 27:1025

    Google Scholar 

  • Thomas E, King PJ, Potrykus I (1977) Naturwissenschaften 64:587

    Google Scholar 

  • Vasil IK (1988) Bio/Technology 6:397–402

    Google Scholar 

  • Vasil V, Vasil IK (1980) Theoret Appl Genet 56:97–99

    Google Scholar 

  • Wernicke W, Brettell RIS (1980) Nature 287:138–139

    Google Scholar 

  • Zhou WB (1978) Acta Genet Sinica 5:337–338

    Google Scholar 

Download references

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Authors and Affiliations

  1. Plant Molecular Genetics Laboratory, Shanghai Institute of Plant Physiology, Academia Sinica, 300 Fenglin Road, 200032, Shanghai, People's Republic of China

    Zhi-ming Wei & Zhi-hong Xu

Authors
  1. Zhi-ming Wei
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  2. Zhi-hong Xu
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Communicated by I.K. Vasil

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Wei, Zm., Xu, Zh. Regeneration of fertile plants from embryogenic suspension culture protoplasts of Sorghum vulgare . Plant Cell Reports 9, 51–53 (1990). https://doi.org/10.1007/BF00232135

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  • DOI: https://doi.org/10.1007/BF00232135

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