Abstract
Mature embryos of Acanthopanax senticosus explanted on Murashige and Skoog (MS) medium with 0.5 mg/1 2,4-D developed somatic embryos directly from swollen cotyledon and embryo axes within one to two months. When the somatic embryos were transferred to medium supplemented with 2,4-D (0.5 mg/1) or IAA (1–3 mg/1) or Zeatin (0.5 mg/1) and NAA (0.2 mg/1), additional somatic embryos developed. Most (93%) embryos germinated on the above medium without 2,4-D. Sixty-two percent of the plantlets survived in soil. Histological observations revealed that the somatic embryos originated from cell masses of epidermal and sub-epidermal origin. There was no cytological separation zone between the somatic embryos and cultured expiants. Consequently, embryos were difficult to separate from their expiant tissue.
Similar content being viewed by others
References
Chang WC, Hsing YZ (1980) Theor Appl Genet 57:133–135.
Cheng J, Raghavan V (1985) Amer J Bot 72(4):580–587.
Gui Y, Guo Z, Ting-yu X, Shurong G (1987) Acta Bot. Sinica 29(2):223–224.
Gui Y, Ting-yu X (1982) Acta Bot. Sinica 24(4):301–306
Gui Y, Ting-yu X, Shurong G, Shuqiong L (1988) Wuhan Bot Res. 6(4):395–397.
Gui Y, Xi-jin M, Ting-yu X (1982) Acta Bot. Sinica 24(3):216–221
Jhang JJ, Staba EJ, Kim JY (1974) In Vitro 9:253–259.
Ke S, Gui Y, Kuo C (1989) Chinese Bulletin of Botany 6(4):205–210.
Kim YH, Janick J (1989a) HortScience 24:674–676.
Kim YH, Janick J (1989b) HortScience 24:671–673.
Kitto SL, Janick J (1985) J Amer Soc Hort Sci 110(2):277–282.
Konar RN, Nataraja K (1965) Phytomorph 15:132–137.
Kuo C, Lu W, (1984) Acta Bot Sinica 26(1):19–23.
Murashige T, Skoog F (1962) Physiol Planta 15:473–497.
Redenbaugh K, Paasch BD, Nichol JW, Kossler ME, Viss PR, Wolker KA (1986) Bio/Tech 4:797–801.
Steward FC, Mapes MO, Mears K (1958) Amer J Bot 45:709–713.
Thompson GA, McCown BH (1986) HortScience 21(3):784 (Abstr.)
Wang D, Guo S, Cheng H (1983) Plant Physiol Communic 7(3):44–48.
Zhang S, Zheng G (1986) Acta Bot Sinica 28(3):241–244.
Zhou J (1981) J Plant Physiol (China) 7:389–397.
Author information
Authors and Affiliations
Additional information
Communicated by J.M. Widholm
Rights and permissions
About this article
Cite this article
Gui, Y., Guo, Z., Ke, S. et al. Somatic embryogenesis and plant regeneration in Acanthopanax senticosus . Plant Cell Reports 9, 514–516 (1991). https://doi.org/10.1007/BF00232108
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232108