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Genetic transformation of Chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity

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Summary

To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few genotype/strain combinations resulted in significant tumour formation. Especially 0-type strains were highly efficient. An 0-type strain was used to transfer genes for neomycine phosphotransferase (NPT II) and ß-glucuronidase (GUS) to a susceptible cultivar. Transfer of the GUS gene was confirmed by using the Polymerase Chain Reaction (PCR).

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Author notes

  1. Hans B. M. Huitema

    Present address: Department of Plant Physiology, University of Agriculture, Arboretumlaan 4, 6703, BD Wageningen, The Netherlands

Authors and Affiliations

  1. Centre for Plant Breeding Research (CPO), P.O.box 16, 6700, AA Wageningen, The Netherlands

    Monique F. van Wordragen, Jan de Jong, Hans B. M. Huitema & Hans J. M. Dons

Authors
  1. Monique F. van Wordragen
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  2. Jan de Jong
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  3. Hans B. M. Huitema
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  4. Hans J. M. Dons
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Communicated by H. Lörz

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van Wordragen, M.F., de Jong, J., Huitema, H.B.M. et al. Genetic transformation of Chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity. Plant Cell Reports 9, 505–508 (1991). https://doi.org/10.1007/BF00232106

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  • DOI: https://doi.org/10.1007/BF00232106

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