Skip to main content
SpringerLink
Log in

Rescuing abortive inter-EBN potato hybrids through double pollination and embryo culture

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Summary

Regeneration of inter-EBN hybrids among potato species was achieved using embryo rescue techniques. Tetraploid hybrids between 4x(2EBN) Solanum stoloniferum x 4x(4EBN) S. tuberosum Gp. Andigena as well as diploid hybrids between 2x(1EBN) S. chancayense x 2x(2EBN) S. chacoense were obtained by culturing immature hybrid embryos in nutrient medium. Identification of appropriate embryo developmental stages was critical in developing a suitable protocol for rescuing viable hybrid embryos. The use of IVP clones as the second pollinator in 4x(2EBN) x 4x(4EBN) crosses reduced premature fruit drop and helped to identify triploid hybrids. Morphological and cytological examination confirmed true hybridity for a few of the regenerated plants. Male sterility and meiotic abnormalities were characteristic of the hybrids. Several S. stoloniferum-Andigena hybrids were successfully backcrossed to Gp. Andigena.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Austin S, Baer MA, Helgeson JP (1985) Plant Sci 39:75–82

    Google Scholar 

  • Barsby TL, Shepard JF, Kemble RJ, Wong R (1984) Plant Cell Rep 3:165–167

    Google Scholar 

  • Bhojwani SS, Razdan WK (1986) Plant tissue culture: theory and practice, Elsevier Science Publishers, Amsterdam, p 199–235

    Google Scholar 

  • Brown CR (1988) Amer Potato J 65:75–84

    Google Scholar 

  • Chavez R, Brown CR, Iwanaga M (1988) Theor Appl Genet 76:129–135

    Google Scholar 

  • Chujoy JE (1985) Ph.D. Thesis, Univ Wisconsin, Madison, 129 pp

    Google Scholar 

  • Fish N, Karp A, Jones MGK (1988) Theor Appl Genet 76:260–266

    Google Scholar 

  • Frandsen NO (1968) Zuchter 38:153–167

    Google Scholar 

  • Hanneman RE Jr, Bamberg JB (1986) Univ Wis Exp Stn Bull 533, 216 pp

  • Haynes FL (1954) Amer Potato J 31:282–288

    Google Scholar 

  • Johnston SA, den Nijs TPM, Peloquin SJ, Hanneman RE Jr (1980) Theor Appl Genet 61:161–169

    Google Scholar 

  • Lee JH, Cooper DC (1958) Amer Potato J 45:104–110

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Ross H (1986) Potato breeding — problems and perspectives, Verlag Paul Parley, Berlin and Hamburg, 130 pp

    Google Scholar 

  • Singsit C, Hanneman RE Jr (1990) Amer Potato J 67:578 (Abstr)

    Google Scholar 

  • Singsit C, Veilleux RE (1989) Euphytica 43:105–112

    Google Scholar 

  • Swaminathan MS (1951) Amer Potato J 28:472–489

    Google Scholar 

  • von Wangenheim KH (1954) Z Plflanzenzucht 34:7–48

    Google Scholar 

  • von Wangenheim KH (1957) Z Indukt Abstamm Vererb 88:21–37

    Google Scholar 

  • Watanabe K, Arbizu C, Schmiediche P, Jackson MT (1990) Amer Potato J 67:586 (Abstr)

    Google Scholar 

  • Williams E, De Lautour G (1980) Bot Gaz 141:252–257

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Horticulture, Vegetable Crops Research Unit, University of Wisconsin, 53706, Madison, WI, USA

    C. Singsit & R. E. Hanneman Jr.

  2. USDA, Agricultural Research Service, Department of Horticulture, University of Wisconsin, 53706, Madison, WI, USA

    R. E. Hanneman Jr.

Authors
  1. C. Singsit
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. E. Hanneman Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. C. Phillips

Cooperative investigation of Vegetable Crops Research Unit, USDA, Agricultural Research Service, and the Wisconsin Agricultural Experiment Station

Note: Reference to a specific brand or firm name does not constitute endorsement by the U.S. Department of Agriculture over others of similar nature not mentioned

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singsit, C., Hanneman, R.E. Rescuing abortive inter-EBN potato hybrids through double pollination and embryo culture. Plant Cell Reports 9, 475–478 (1991). https://doi.org/10.1007/BF00232099

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232099

Keywords

Search

Navigation