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Association of folic acid with rat liver microsomes

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Summary

Shin et al. (Biochim Biophys Acta 444: 794–801, 1976) described the subcellular location of [3H]folic acid after injection into rats. The microsomal fraction of the liver contained relatively large amounts of tracer initially but lower amounts at later times. Because of the heterogeneous nature of the microsomal fraction of the liver we re-examined the nature of the folate binding fraction. The location of injected [3H]folic acid resembled that of the microsomes derived from the plasma membrane, where ultracentrifugal analysis was conducted in the presence and absence of cesium ions. The location of the folate did not resemble that of microsomes derived from the endoplasmic reticulum (ER). One of the marker enzymes of the ER was the vitamin K-dependent carboxylase. A simple method for reducing vitamin K is described.

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References

  1. Brody T, Shane B, Stokstad ELR: Folic acid. In: L Machlin (ed) Handbook on Vitamins. Marcel Dekker, Inc, New York, 1984, pp 459–496

    Google Scholar 

  2. Shin YS, Williams MA, Stokstad ELR: Identification of folic acid compounds in rat liver. Biochem Biophys Res Commun 47: 35–43, 1972

    Google Scholar 

  3. Houilihan CM, Scott J: The identification of pteroylpentaglutamate as the major folate derivative in rat liver and the demonstration of its biosynthesis from exogenous [3H]pteroylglutamate. Biochem Biophys Res Commun 48: 1675–1681, 1972

    Google Scholar 

  4. Corrocher R, Hoffbrand AV: Subcellular localization of folate and effect of methotrexate on the incorporation of radioactive folic acid into guinea pig liver folate. Clinical Sci 43: 815–822, 1972

    Google Scholar 

  5. Shin YS, Chan C, Vidal A, Brody T, Stokstad ELR: Subcellular localization of γ-glutamyl carboxypeptidase and of folates. Biochim Biophys Acta 444: 794–801, 1976

    Google Scholar 

  6. Cook RJ, Blair JA: The distribution and chemical nature of radioactive folates in rat liver cells and rat liver mitochondria. Biochem J 178: 651–659, 1979

    Google Scholar 

  7. Zamierowski MM, Wagner C: Identification of folate binding proteins in rat liver. J Biol Chem 252: 933–938, 1977

    Google Scholar 

  8. Chicheportiche Y, Vassalli P, Tartakoff AM: Characterization of cytoplasmically oriented golgi porteins with a monoclonal antibody. J Cell Biology 99: 2200–2210, 1984

    Google Scholar 

  9. Pletsch QA, Coffrey JW: Intracellular distribution of radioactive vitamin B12 in rat liver. J Biol Chem 246: 4619–4629, 1971

    Google Scholar 

  10. Marshall S, Heidenreich KA, Horikoshi H: Stoichiometric translocation of adipocyte insulin receptors from the cell-surface to the cell-interior. J Biol Chem 260: 4128–4135, 1985

    Google Scholar 

  11. Carpentier JL, Dayer JD, Lang U, Silverman R, Orci L, Gorden P: Down-regulation and recycling of insulin receptors. J Biel Chem 259: 14190–14195, 1984

    Google Scholar 

  12. Oliver JM, Ukena TE, Berlin RD: Effects of phagocytosis and colchicine on the distribution of lectin-binding site on cell surfaces. Proc Natl Acad Sci USA 71: 394–398, 1974

    Google Scholar 

  13. Thilo L: Selective internationalization of granule membrane after secretion in mast cells. Proc Natl Acad Sci USA 82: 1711–1715, 1985

    Google Scholar 

  14. Henderson GB, Grzelakowska-Sztabert B, Zevely EM, Huennekens FM: Binding properties of the 5-methyltetrahydrofolate/methotrexate transport system in L1210 cells. Arch Biochem Biophys 202: 144–149, 1980

    Google Scholar 

  15. Henderson GB, Zevely EM: Affinity labeling of the 5-methyltetrahydrofolate/methotrexate transport protein of L1210 cells by treatment with an N-hydroxysuccinimide ester of [3H]methotrexate. J Biol Chem 259: 4558–4562, 1984

    Google Scholar 

  16. Kamen BA, Capdevila A: Receptor-mediated folate accumulation is regulated by the cellular folate content. Proc Natl Acad Sci USA 83: 5983–5987, 1986

    Google Scholar 

  17. Antony AC, Kane M, Portillo RM, Elwood PC, Kolhouse JF: Studies of the role of a particulate folate-binding protein in the uptake of 5-methyltetrahydrofolate by cultured human KB cells. J Biol Chem 260: 14911–14917, 1985

    Google Scholar 

  18. Kamen BA, Wang MT, Streckfuss AJ, Peryea X, Anderson RGW: Delivery of folates to the cytoplasm of MA104 cells is mediated by a surface membrane receptor that recycles. J Biol Chem 263: 13602–13609, 1988

    Google Scholar 

  19. McHugh M, Cheng YC: Demonstration of a high affinity folate binder in human cell membranes and its characterization in cultured human KB cells. J Biol Chem 254: 11312–11318, 1979

    Google Scholar 

  20. Selhub J, Franklin WA: The folate-binding protein of rat kidney. J Biol Chem 259: 6601–6606, 1984

    Google Scholar 

  21. Thenen SW, Stokstad ELR: Effect of methionine on specific folate coenzyme pools in vitamin B12 deficient and supplemented rats. J Nurt 103: 363–370, 1973

    Google Scholar 

  22. Bohlen P, Stein S, Dairman W, Udenfriend S: Fluoremetric assay of proteins in the nanogram range. Arch Biochem Biophys 155: 213–220, 1973

    Google Scholar 

  23. Dallner G: Isolation of rough and smooth microsomes. In: S Fleischer and L Packer (eds) Methods in Enzymology Vol. 31, Part A, Academic Press, New York, 1974, pp 191–201

    Google Scholar 

  24. Bergstrand A, Dallner G: Isolation of rough and smooth microsomes from rat liver by means of a commercially available centrifuge. Anal Biochem 29: 351–356, 1969

    Google Scholar 

  25. Aronson NN, Touster O: Isolation of rat liver plasma membrane fragments in isotonic sucrose. In: S Fleischer and L Packer (eds) Methods in Enzymology Vol. 31, Part A, Academic Press, New York, 1974, pp. 90–112

    Google Scholar 

  26. Beafay H, Amar-Costesec A, Feytmans E, Thines-Sempoux D, Wibo M, Robbi M, Berthet J: Analytical study of microsomes and isolated subcellular membranes from rat liver. J Cell Biol 61: 188–200, 1974

    Google Scholar 

  27. Ray TK: A modified method for the isolation of the plasma membrane from rat liver. Biochem Biophys Acta 196: 1–9, 1970

    Google Scholar 

  28. Cooperstein SJ, Lazarow A: A microspectrophotometric method for the determination of cytochrome oxidase. J Biol Chem 189: 665–670, 1951

    Google Scholar 

  29. Carlisle TL, Suttie JW: Vitamin K dependent carboxylase. Biochemistry 19: 1161–1167, 1980

    Google Scholar 

  30. Brody T, Suttie JW: Glutamate carboxylase. In: F Fold and K Moldave (eds) Methods in Enzymology Vol. 107, Academic Press, New York, 1984, pp. 552–563

    Google Scholar 

  31. Fieser LF: Synthesis of vitamin K1. J Am Chem Soc 61: 3467–3475, 1939

    Google Scholar 

  32. Fasco MJ, Principle LM: Vitamin K1 hydroquinone formation catalyzed by a microsomal reductase system. Biochem Biophys Res Commun 97: 1487–1492, 1980

    Google Scholar 

  33. Shin YS, Buehring KU, Stokstad ELR: Separation of folid acid compounds by gel chromatography on Sephadex G-15 and G-25. J Biol Chem 247: 7266–7269, 1972

    Google Scholar 

  34. Garfield SA, Cardell RR: Hepatic glucose-6-phosphatase activities and correlated ultrastructural alterations in hepatocytes of diabetic rats. Diabetes 28: 664–679, 1979

    Google Scholar 

  35. Wibo M, Thines-Sempoux D, Amar-Costesec A, Beaufay H, Godelaine D: Analytical study of microsomes and isolated subcellular membranes from rat liver. J Cell Biol 89: 456–474, 1981

    Google Scholar 

  36. Brody T, Suttie JW: Evidence for the glycoprotein nature of the vitamin K-dependent carboxylase. Biochim Biophys Acta 923: 1–7, 1987

    Google Scholar 

  37. Sabesin SM, Frase S; Electron microscopic studies of the assembly, intracellular transport, and secretion of chylomicrons by rat intestine. J Lipid Res 18: 496–511, 1977

    Google Scholar 

  38. Seetharam B, Alpers DH: Absorption and transport of cobalamin. Ann Rev Nutr 2: 343–369, 1982

    Google Scholar 

  39. Mellman I, Plutner H, Ukkonen P: Internationalization and rapid recyling of macrophage Fc receptors tagged with monovalent antireceptor antibody. J Cell Biol 98: 1163–1169, 1984

    Google Scholar 

  40. Berger M, Birx DL, Wetzler EM, O'Shea JJ, Brown EJ, Cross AS: Calcium requirements for increased complement receptor expression during neutrophil activation. J Immunol 135: 1342–1348, 1985

    Google Scholar 

  41. Karnieli E, Zarnowski MJ, Hissin PJ, Simpson IA, Salans LB, Cushman SW: Insulin-stimulated translocation of glucose transport systems in the isolated rat adipocyte cell. J Biol Chem 256: 4772–4777, 1981

    Google Scholar 

  42. Forte JG, Black JA, Forte TM, Machen TE, Wolosin JM: Ultrastructural changes related to functional activity in gastric oxyntic cells. Am J Physiol 241: G349-G358, 1981

    Google Scholar 

  43. Shane B, Stokstad ELR: Transport and metabolism of folates by bacteria. J Biol Chem 250: 2243–2253, 1975

    CAS  PubMed  Google Scholar 

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  1. Dept. of Nutritional Sciences, Univ. of California at Berkeley, 94720, Berkeley, CA, USA

    Tom Brodyt & E. L. R. Stokstadt

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  1. Tom Brodyt
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  2. E. L. R. Stokstadt
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Brodyt, T., Stokstadt, E.L.R. Association of folic acid with rat liver microsomes. Mol Cell Biochem 97, 67–73 (1990). https://doi.org/10.1007/BF00231702

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  • DOI: https://doi.org/10.1007/BF00231702

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