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Synthesis of Ogura male sterile rapeseed (Brassica napus L.) with cold tolerance by protoplast fusion and effects of atrazine resistance on seed yield

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Abstract

Twenty-one cold-tolerant, male sterile Brassica napus somatic hybrids were produced by protoplast fusion. The fusion partners were a coldsensitive, Ogura cytoplasmic male sterile cauliflower inbred (B. oleracea var. botrytis inbred NY7642A) and a cold-tolerant, fertile canola-type B. rapa cv. Candle. Hybridity was confirmed by morphology, isozyme expression, flow cytometry, and DNA hybridization. Organellar analyses revealed a very strong bias for Brassica over Raphanus chloroplasts. Cold tolerance was confirmed by cold chamber studies and chloroplast DNA analyses. Good female fertility with 21.4 ± 3.1 seeds/pod was observed in the field using natural pollination vectors. Total seed yield was significantly greater for the atrazine-sensitive somatic hybrids produced in this study than for atrazine-resistant isolines.

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Abbreviations

CMS:

cytoplasmic male sterility

IA:

iodoacetate

cpDNA:

chloroplast DNA

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Author notes

  1. Douglas W. Heath

    Present address: Petoseed Co., Inc., 37437 St. Hwy 16, 95695, Woodland, CA, USA

Authors and Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA

    Douglas W. Heath & Elizabeth D. Earle

Authors
  1. Douglas W. Heath
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  2. Elizabeth D. Earle
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Communicated by C. Quiros

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Heath, D.W., Earle, E.D. Synthesis of Ogura male sterile rapeseed (Brassica napus L.) with cold tolerance by protoplast fusion and effects of atrazine resistance on seed yield. Plant Cell Reports 15, 939–944 (1996). https://doi.org/10.1007/BF00231592

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  • DOI: https://doi.org/10.1007/BF00231592

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