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Inhibition of prostaglandin synthase activity of sheep seminal vesicular gland by human serum haptoglobin

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Summary

Serum haptoglobin was added to the reaction mixture of prostaglandin synthase (EC 1.14.99.1) and its inhibitory effect was studied. [1-14C]Arachidonic acid was used as substrate and the enzyme activity was estimated by monitoring the radioactivity of the products after thin layer chromatography. With or without addition of hemoglobin to the reaction mixture, both the purified haptoglobin 1-1 and 2-2 showed inhibitory activity. In the presence of 5 μM hematin, however, inhibitory activity of haptoglobin was not observed. Inhibition of prostaglandin synthesis in the system depended on the molar ratio of haptoglobin to hemoglobin in the reaction mixture. These results demonstrate that haptoglobin inhibits prostaglandin synthase by restricting available heme group for the enzyme activity through complexing with hemoglobin. However, haptoglobin did not inhibit completely the stimulatory effect of free hemoglobin. Relevant significance of this effect was discussed.

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Abbreviations

Hp:

haptoglobin

EIPS:

endogenous inhibitor of prostaglandin synthase

Hb:

hemoglobin

Hp-Hb:

haptoglobin-hemoglobin complex

References

  1. Lands, W. E. M. and Samuelsson, B., 1968. Biochem. Biophys. Acta 164: 426–429.

    Google Scholar 

  2. Bergström, S., Carlson, L. A. and Weeks, J. R., 1968. Pharmacol. Rev. 20: 1–40.

    Google Scholar 

  3. Ramwell, P. W., Shaw, J. E., Clarke, G. B., Grostic, M. F., Keiser, D. G. and Pike, J. E., 1968. Prog. Chem. Fat Other Lipids 9: 233–273.

    Google Scholar 

  4. Hemler, M. and Lands, W. E. M., 1976. J. Biol. Chem. 251: 5575–5579.

    Google Scholar 

  5. Miyamoto, T., Ogino, M., Yamamoto, S. and Hayaishi, O., 1976. J. Biol. Chem. 251: 2629–2636.

    Google Scholar 

  6. Van der Ouderaa, F. J., Buytenhek, M., Nugteren, D. H. and Van Dorp, D. A., 1977. Biochim. Biophys. Acta 487: 315–331.

    Google Scholar 

  7. Ogino, N., Miyamoto, T., Yamamoto, S. and Hayaishi, O., 1977. J. Biol. Chem. 252: 890–895.

    Google Scholar 

  8. Christ-Hazelhof, E. and Nugteren, D. H., 1979. Biochim Biophys. Acta 572: 43–51.

    Google Scholar 

  9. Shimizu, T., Yamamoto, S. and Hayaishi, O., 1979. J. Biol. Chem. 254: 5222–5228.

    Google Scholar 

  10. Roth, G. J., Sick, C. J. and Ozols, J., 1980. J. Biol. Chem. 255: 1301–1304.

    Google Scholar 

  11. Shim, B.-S., 1976. Korean J. Biochem. 8: 7–11.

    Google Scholar 

  12. Saeed, S. A., McDonald-Gibson, W. J., Cuthbert, J., Copas, J. L., Schneider, C., Butt, N. M. and Collier, H. O. J., 1977. Nature (London) 270: 32–36.

    Google Scholar 

  13. Vane, J. R., 1971. Nature (London) New Biol. 231: 232–235.

    Google Scholar 

  14. Kang, Y.-S., Jue, D.-M. and Shim, B.-S., 1979. Korean J. Biochem. 10: 64–65.

    Google Scholar 

  15. Kang, Y.-S., Jue, D.-M. and Shim. B.-S., 1979. Korean J. Biochem. 11: 21–25.

    Google Scholar 

  16. Benesch, R. E., Benesch, R., Renthal, R. D. and Maeda, N., 1972. Biochemistry 11: 3576–3582.

    Google Scholar 

  17. Chu, T. C. and Chu, E. J.-H., 1955. J. Biol. Chem. 212: 1–7.

    Google Scholar 

  18. Wallach, D. P. and Daniels, E. G., 1971. Biochim. Biophys. Acta 231: 445–447.

    Google Scholar 

  19. Han, S.-M. and Shim, B.-S., 1965. Korean Central J. Med. 9: 453–460.

    Google Scholar 

  20. Shim, B.-S., Park, Y.-H., Kim, Y.-J. and Lee, T.-H., 1959. J. Cath. Med. Coll. (Seoul) 3: 176–182.

    Google Scholar 

  21. Cameron, B. F., 1965. Anal. Biochem. 11: 164–169.

    Google Scholar 

  22. Paul, K. G., Theorell, H. and Akeson, A., 1953. Acta Chem. Scand. 7: 1284–1287.

    Google Scholar 

  23. Lowry, O. H., Rosenberg, H. J., Farr, A. L. and Randall, R. J., 1951. J. Biol. Chem. 193: 265–275.

    CAS  PubMed  Google Scholar 

  24. Shim, B.-S. and Bearn, A. G., 1964. J. Exp. Med. 120: 611–628.

    Google Scholar 

  25. Shim, B.-S., Lee, T.-H. and Kang, Y.-S., 1965. Nature(London) 207: 1264–1267.

    Google Scholar 

  26. Shim, B.-S., 1973. Genetics, Structure and Function of Serum Haptoglobin pp. 10–25, Catholic Medical College, Seoul.

    Google Scholar 

  27. Van der Ouderaa, F. J., Buytenhek, M., Slikkerveer, F. J. and Van Dorp, D. A., 1979. Biochim. Biophys. Acta 572: 29–42.

    Google Scholar 

  28. Waks, M., Alfsen, A., Schwaiger, S. and Mayer, A., 1969. Arch. Biochem. Biophys. 132: 268–278.

    Google Scholar 

  29. Hong, K.-J., Kim, S.-K., Choi, J.-M., Cho, B.-K. and Shim, B.-S., 1973. Korean Central J. Med. 25: 205–211.

    Google Scholar 

  30. Deby, C., Van Caneghem, P. and Bacq, Z. M. 1978. Biochem. Pharmacol. 27: 613–615.

    Google Scholar 

  31. Shim, B.-S., 1976. Nature (London) 259: 326–327.

    Google Scholar 

  32. Hong, K.-J. and Shim, B.-S., 1977. Korean J. Biochem. 9: 5–13.

    Google Scholar 

  33. Shim, B.-S. and Kim, I.-K., 1978. Vox Sang. 35: 414–419.

    Google Scholar 

  34. Horrobin, D. F., 1978. Prostaglandins: Physiology, Pharmacology and Clinical Significance, Churchill Livingstone, Edinburgh, Scotland.

    Google Scholar 

  35. Weinberg, J. B. and Hibbs, J. B., Jr., 1977. Nature(London) 269: 245–247.

    Google Scholar 

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Author notes

  1. Yoon-Se Kang

    Present address: Department of Biochemistry, Kyungsang University, 620, Jinju, Korea

Authors and Affiliations

  1. Department of Biochemistry, Catholic Medical College, 110, Seoul, Korea

    Dae-Myung Jue, Bong-Sop Shim & Yoon-Se Kang

Authors
  1. Dae-Myung Jue
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  2. Bong-Sop Shim
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Jue, DM., Shim, BS. & Kang, YS. Inhibition of prostaglandin synthase activity of sheep seminal vesicular gland by human serum haptoglobin. Mol Cell Biochem 51, 141–147 (1983). https://doi.org/10.1007/BF00230400

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  • DOI: https://doi.org/10.1007/BF00230400

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