Summary
Glucagon increased alanine amino transferase (AAT) activity in perfused rat liver by about 90% over control. Propranolol, the beta receptor antagonist, abolished the effect of glucagon on this enzyme. Well known beta receptor agonists like isoproterenol, norepinephrine and epinephrine also increased the enzyme activity under identical condition and the enhancement was similarly abolished by propranolol. These experiments suggest that the effect of glucagon on AAT was mediated through beta adrenergic receptor. However, the interesting observation was that phenylephrine, alpha receptor agonist and phenoxybenzamine and tolazoline, two alpha receptor antagonists, increased the AAT activity like glucagon in perfusion experiments and the effects of all these three agents were also abolished by propranolol. Glucagon, when perfused with phenoxybenzamine showed some additive effect. From all these results we are proposing that in our system phenoxybenzamine is acting as beta agonist although it is known to be an alpha antagonist.
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References
Exton JH, Park CR: Control of gluconeogenesis in liver. J Biol Chem 244: 1424–1433, 1969
Pilkis SJ, Fox E, Wolfe L, Rothbarth L, Colosia A, Stewart HB, El-MaghrabiMR: Hormonal modulation of key hepatic regulatory enzymes in the gluconeogenic/glycolytic pathway. Annals New York Academy of Sciences 478: 1–19, 1986
Riou JP, Claus TH, Pilkis SJ: Stimulation by glucagon in vivo phosphorylation of rat hepatic pyruvate kinase. J Biol Chem 253: 656–659, 1978
Garrison JC, Haynes RC Jr: The hormonal control of gluconeogenesis by regulation of mitochondrial pyruvate carboxylation in isolated rat liver cells. J Biol Chem 250: 2769–2777, 1975
Chatterjee T, Datta AG: Effect of glucagon on mice liver fructose 1,6 bisphosphatase. Biochem Biophys Res Commun 84: 950–956, 1978
Claus TH, Schlumpf J, El-Maghrabi MR, McGrane M, Pilkis SJ: Glucagon stimulation of fructose-1,6-bisphosphatase phosphorylation in rat hepatocytes. Biochem Biophys Res Commun 100: 716–723, 1981
Felig P, Pozefsky T, Marliss E, Cahill GF Jr: Alanine; Key role in gluconeogenesis. Science 167: 1003–1004, 1970
Chiasson JL, Cook J, Liljenquist JE, Lacy WW: Glucagon stimulation of gluconeogensis from alanine in the intact dog. Am J Physiol 227: 19–23, 1974
Mallete LE, Exton JH, Park CR: Control of gluconeogenesis from amino acids in the perfused rat liver. J Biol Chem 244:5713–5723, 1969
Joseph SK, McGivan JD: The effect of ammonium chloride and glucagon on the metabolism of glutamine in isolated liver cells from starved rat. Biochim Biophys Acta 543: 16–28, 1978
Exton JH, Robison JA, Sutherland EW, Park CR: Studies on the role of 3′5′ monophosphate in the hepatic actions of glucagon. J Biol Chem 246: 6166–6177, 1971
McGivan JD, Ramsell JC, Lacy JH: Stimulation of alanine transport and metabolism by dibutyryl cyclic AMP in the hepatocytes from fed rat. Biochim Biophys Acta 644: 295–304, 1981
Greenberg DM: Carbon catabolism of amino acids. In: Metabolic Pathways. Academic Press, New York, 1969, pp 95–190, Vol 3
Banerjee RB, Bhadra R, Datta AG: Effect of glucagon on alanine-2-oxoglutarate aminotransferase. Biochem Biophys Res Commun 115: 506–511, 1983
Exton JH, Park CR: Control of gluconeogenesis in liver. J Biol Chem 242: 2622–2636, 1967
De Roosa G, Swick RW: Metabolic implications of the distribution of the AAT isozymes. J Biol Chem 200: 7961–7967, 1975
Segal JL, Matsuzawa T: L-alanine aminotransferase (rat liver). In: H Tabor and GW Tabor (eds) Methods of Enzymology. Academic Press, New York and London, 1970, pp 153–159, Vol 17 A
Stadel JM, Lefkowitz RL: The beta adrenergic receptor: Ligand binding studies illuminate the mechanism of receptor-adenylate cyclase coupling. In: A Kleinzeller and BR Martin (eds) Current Topics in Membrane and Transport. Academic Press, INC (London), 1983, pp 45–66, Vol 18
Gullapali S, Shivaswamy V, Ramasarma T, Kurup CKR: Redistribution of subcellular calcium in rat liver on administration of vanadate. Mol Cell Biochem 90: 155–154, 1989
Taunton OD, Stifel FB, Greene HL, Herman RH: Rapid reciprocal changes of rat hepatic glycolytic enzymes and fructose-1,6-bisphosphatase following glucagon and insulin injection in vivo. Biochem Biophys Res Commun 48: 1663–1670, 1972
Exton JH: Mechanisms involved in effects of catecholamines on liver carbohydrate metabolism. Biochem Pharmacol 28: 2237–2240, 1979
Weiner N: Drugs that inhibit adrenergic nerves and block adrenergic receptors. In: AG Gilman, LS Goodman and A Gilman (eds) The Pharmacological Basis of Therapeutics. Macmillan Publishing Co., INC, 1980, pp 176–210, Edition 6
Houslay MD, Metcalfe JC, Warrent GB, Hesketh TR, Smith GA: The glucagon receptor of rat liver in plasma membrane can couple to adenylate cyclase without activating it. Biochim Biophys Acta 436: 489–494, 1976
Garrison JC, Borland MK: Regulation of mitochondrial pyruvate carboxylation and gluconeogenesis in rat hepatocytes via an alpha adrenergic, adenosine 3'5' monophosphate-independent mechanism. J Biol Chem 254: 1129–1133, 1979
Pariza MW, Butcher FR, Becker JE, Potter VR: 3′5′ cyclic AMP: Independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells. Proc Natl Acad Sci USA 74: 234–238, 1977
Le Cam A, Freychet P: Effect of catecholamines on amino acid transport in isolated rat hepatocytes. Endocrinology 102:379–385, 1978
Houslay MD: A better view of beta receptors. TIBS 5: 208–209, 1984
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Begum, N.A., Datta, A.G. Effect of glucagon and some other alpha and beta adrenergic agonists and antagonists on alanine amino transferase of perfused rat liver. Mol Cell Biochem 105, 7–13 (1991). https://doi.org/10.1007/BF00230369
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DOI: https://doi.org/10.1007/BF00230369