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Alterations in human gingival glycosaminoglycan pattern in inflammation and in phenytoin induced overgrowth

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Abstract

Glycosaminoglycans were extracted from normal, inflamed and phenytoin induced overgrowth of human gingival tissue by proteolysis and alcohol precipitation. Extracts were run in a Dowex-1 column and the fractions were treated with mucopolysaccharidases. Cellulose acetate electrophoresis was carried out with or without enzyme digestion for identification of individual glycosaminoglycans. Glycosaminoglycans were found to be decreased in inflammation but were observed to increase in the overgrowth. Hyaluronic acid was found to be increased in both the pathological conditions. Dermatan sulphate, chondroitin sulphate and heparan sulphate were observed to be decreased in inflammation. In overgrowth, dermatan sulphate and chondroitin sulphate were found to increase while the presence of heparan sulphate was not significant. The changes in the pattern of individual glycosaminoglycan in the two varied conditions are discussed.

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Abbreviations

GAG:

glycosaminoglycan

MPS:

mucopolysaccharide

DS:

dermatan sulphate

HS:

heparan sulphate

CS:

chondroitin sulphate

HA:

hyaluronic acid

KS:

keratan sulphate

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Authors and Affiliations

  1. Department of Biochemistry, Central Leather Research Institute, 600 020, Adyar, Madras, India

    R. Suresh, R. Puvanakrishnan & S. C. Dhar

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  1. R. Suresh
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  2. R. Puvanakrishnan
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  3. S. C. Dhar
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Suresh, R., Puvanakrishnan, R. & Dhar, S.C. Alterations in human gingival glycosaminoglycan pattern in inflammation and in phenytoin induced overgrowth. Mol Cell Biochem 115, 149–154 (1992). https://doi.org/10.1007/BF00230325

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  • DOI: https://doi.org/10.1007/BF00230325

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