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Dual anomeric specificity of phosphomannoisomerase assessed by 2D phase sensitive 13C EXSY NMR

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Abstract

The reversible conversion between D-mannose 6-phosphate and D-fructose 6-phosphate catalyzed by yeast phosphomannoisomerase was studied by phase sensitive 2D 13C-1H EXSY NMR spectroscopy at 100.623 MHz, using 13C enriched substrates in the C2 position of the D-hexose 6-phosphates. The unique pair of isomerization cross-peaks observed in the 2D EXSY map correlates the 13C2 resonances of the β-anomers of both D-[2-13C]-mannose-6-phosphate and D-[213C]-fructose 6-phosphate. This indicates that phosphomannoisomerase specifically catalyzes the reversible conversion between β-D-mannose 6-phosphate and β-D-fructose 6-phosphate. Since phosphoglucoisomerase was recently found to catalyze specifically the interconversion of α-D-glucose 6-phosphate and β-D-fructose 6-phosphate, the β-anomer of the ketohexose ester could be directly channeled in a multi-enzyme system involving phosphoglucoisomerase, phosphomannoisomerase and phosphofructokinase.

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Authors and Affiliations

  1. Laboratory of Experimental Medicine, Erasmus Medical School, Brussels Free University (ULB), Belgium

    Francine Malaisse-Lagael & Willy J. Malaisse

  2. Department of General and Organic Chemistry, Faculty of Engineering and High Resolution NMR Center, Free University of Brussels (VUB), Belgium

    Rudolph Willem

  3. Bruker Spectrospin Brussels, Belgium

    Marc Penders

Authors
  1. Francine Malaisse-Lagael
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  2. Rudolph Willem
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  3. Marc Penders
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  4. Willy J. Malaisse
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Malaisse-Lagael, F., Willem, R., Penders, M. et al. Dual anomeric specificity of phosphomannoisomerase assessed by 2D phase sensitive 13C EXSY NMR. Mol Cell Biochem 115, 137–142 (1992). https://doi.org/10.1007/BF00230323

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  • DOI: https://doi.org/10.1007/BF00230323

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