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Partial purification and characterization of cyclic nucleotide phosphodiesterases from human bronchial tissue

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Summary

DEAE-Sephadex chromatography reveals the presence in extracts of human bronchial tissue of at least three separate cyclic nucleotide phosphodiesterases: a cyclic GMP-specific high affinity enzyme, a non-specific low affinity enzyme, and a high affinity cyclic AMP-specific enzyme. The activity of each fraction was partially characterized with respect to kinetic parameters, thermal stability, and the influence of a number of inhibitors. Each activity was found to resemble the activity of the previously characterized corresponding enzyme from whole lung tissue extracts. A high affinity non-specific phospho-diesterase previously isolated from lung tissue is missing in extracts of bronchial tissue.

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References

  1. Appleman, M. M., Thompson, W. J., and Russell, T. R., 1973. Adv. Cyclic Nucleotide Res. 3, 65–98.

    Google Scholar 

  2. Kakiuchi, S., Yamazaki, R., Teshima, Y., Uenishi, K., and Miyamoto, E., 1975. Adv. Cyclic Nucleotide Res. 5, 163–178.

    Google Scholar 

  3. Wang, J. H., Teo, T. S., Ho, H. C., and Stevens, F. C., 1975. Adv. Cyclic Nucleotide Res. 5, 179–194.

    Google Scholar 

  4. Feiss, B., 1975. Adv. Cyclic Nucleotide Res. 5, 195–211.

    Google Scholar 

  5. Goldberg, M. D., and Haddox, M. K., 1977. Ann. Rev. Biochem. 46, 823–896.

    Google Scholar 

  6. Amer, M. S., and Kreighbaum, W. E., 1975. J. Pharm. Sci. 64, 1–37.

    Google Scholar 

  7. Chasin, M., and Harris, D. (1977). Adv. Cyclic Nucleotide Res. 7, 225–264.

    Google Scholar 

  8. Russell, T. R., Terasaki, W. L., and Appleman, M. M. 1973. J. Biol. Chem. 248, 1334–1340.

    Google Scholar 

  9. Bergstrand, H., Lundquist, B., and Schurmann, A. 1977. J. Biol. Chem. (in press).

  10. Bergstrand, H., and Lundquist, B. 1976. Biochemistry 15, 1727–1735.

    Google Scholar 

  11. Thompson, W. J., and Appleman, M. M. 1971. Biochem. 10, 311–316.

    Google Scholar 

  12. Lowry, O. H., Rosebrough, N. J., Farr, C. A., and Randall, R. J. 1951. J. Biol. Chem. 193, 265–275.

    CAS  PubMed  Google Scholar 

  13. Engel, P. C. and Ferdinand, W. 1973. Biochem. J. 131, 97–105.

    Google Scholar 

  14. Segel, I. H. 1975. Enzyme kinetics. John Wiley & Sons, N.Y.

    Google Scholar 

  15. Wilkinson, G. N. 1961. Biochem. J. 80, 324–332.

    Google Scholar 

  16. Wolff, D. J. and Brostrom, C. O. 1976. Arch. Biochem. Biophys. 173, 720–731.

    Google Scholar 

  17. Pichard, A. L., and Cheung, W. Y. 1976. J. Biol. Chem. 252, 4872–4875.

    Google Scholar 

  18. Bergstrand, H., Kristoffersson, J., Lundquist, B., and Schurmann, A. 1977. Mol. Pharmacol. 13, 38–43.

    Google Scholar 

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Authors and Affiliations

  1. The Research and Development Laboratories, AB Draco, Fack, S-221 01, Lund, Sweden

    Håkan Bergstrand & Britta Lundquist

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  1. Håkan Bergstrand
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  2. Britta Lundquist
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Bergstrand, H., Lundquist, B. Partial purification and characterization of cyclic nucleotide phosphodiesterases from human bronchial tissue. Mol Cell Biochem 21, 9–15 (1978). https://doi.org/10.1007/BF00230191

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  • DOI: https://doi.org/10.1007/BF00230191

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