Abstract
The cardiac ventricular myosin heavy chain phenotype is developmentally and hormonally regulated, but less is known concerning the actin phenotype. In this study, the levels of accumulation of α-skeletal and α-cardiac actin mRNAs were investigated in rat and human ventricles by primer extension assays. In rat, the two iso-mRNAs are present in approximately equal amounts from birth until 15 days of age and the cardiac form is predominant in adult and senescent hearts. Hypothyroid development has no effect, at least during the first two weeks of age. In man, the two isoactins are co-expressed to similar ratios in one control heart and in one failing heart. It therefore appears that myosin heavy chain and actin multigene families are both expressed in a species specific fashion but are independently regulated within a species. Preliminary results from nuclear run-on assays are presented that indicate differences in the level of transcription of the α-actin and β-myosin heavy chain isogenes in the rat heart.
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References
Barany M: ATPase activity of myosin correlated with speed of muscle shortening. J Gen Physiol 50: 197–216, 1967
Buller AJ, Mommaerts W, Seraydarian K: Enzymic properties of myosin in fast and slow twitch muscles of the cat following cross-innervation. J Physiol 205: 581–597, 1969
Morgan HE, Gordon EE, Kira Y, Chua BHL, Russo LA, Peterson CJ, McDermott P, Watson PA: Biochemical mechanisms of cardiac hypertrophy. Ann Rev Physiol 49: 533–543, 1987
Bishopric NH, Simpson PC, Ordhal CP: Induction of the skeletal α-actin gene in α 1-adrenoreceptor-mediated hypertrophy of rat cardiac myocytes. J Clin Invest 80: 1194–1199, 1987
Winegrad S, Wisnewsky C, Schwartz K: Effect of thyroid hormone on the accumulation of mRNA for skeletal and cardiac α-actin in hearts from normal and hypophysectomised rats. Proc Natl Acad Sci USA 87: 2456–2460, 1990
Samuel JL, Rappaport L, Syrovy I, Wisnewsky C, Marotte F, Whalen RG, Schwartz K: Differential effect of thyroxine on atrial and ventricular isomyosins in rats. Amer J Physiol 250: H333-H341, 1986
Liew CC, Jackowski G, Ma T, Sole MJ: Nonenzymatic separation of myocardial cell nuclei from whole heart tissue. Am J Physiol 244: C3-C10, 1983
Konieczny SF, Emerson CP: Differentiation, not determination, regulates muscle gene activation: transfection of troponin I genes into multipotential and muscle lineages of 10T1/2 cells. Mol Cell Biol 5: 2423–2432, 1985
Marzluff WF, Huang RCC: Transcription of RNA in Isolated Nuclei. In: BD Hames, SJ Higgins (eds) Transcription and Translation. IRL Press, Washington, 1984, pp 89–129
Sassoon DA, Garner I, Buckingham M: Transcripts of acardiac and α-skeletal actins are early markers for myogenesis in the mouse embryo. Development 104: 155–164, 1988
Schiaffino S, Samuel JL, Sassoon D, Lompre AM, Garner I, Marotte F, Buckingham M, Rappaport L, Schwartz K: Nonsynchronous accumulation of α-skeletal actin and β-myosin heavy chain mRNAs during early stages of pressure-overload-induced cardiac hypertrophy demonstrated by in situ hybridization. Circ Res 64: 937–948, 1989
Long CS, Ordhal CP, Simpson PC: α 1-Adrenergic receptor stimulation of sarcomeric actin isogene transcription in hypertrophy of cultured rat heart muscle cells. J Clin Invest 83: 1078–1082, 1989
Schwartz K, de la Bastie D, Bouveret P, Oliviéro P, Alonso S, Buckingham M: α-skeletal actin mRNA's accumulate in hypertrophied adult rat hearts. Circ Res 59: 551–555, 1986
Mayer Y, Czosnek H, Zeelon P, Yaffe D, Nudel U: Expression of the genes coding for the skeletal muscle and cardiac actins in the heart. Nucleic Acids Res 12: 1087–2000, 1984
Lompré AM, Nasal-Ginard B, Mahdavi V: Expression of the cardiac ventricular α- and β- myosin heavy chain genes is developmentally and hormononally regulated. J Biol Chem 259: 6437–6446, 1984
O'Neill L, Holbrook N, Lakatta EG: Progressive changes in steady state mRNA levels of rat cardiac myosin heavy chain genes from young age to senescence. J Mol Cell Cardiol 22, Suppl I: S34, 1990
Chizzonite RA, Zak R: Regulation of myosin isoenzyme composition in fetal and neonatal rat ventricle by endogenous thyroid hormones. J Biol Chem 259: 12628–12632, 1984
Izumo S, Lompré AM, Matsuoka R, Koren G, Schwartz K, Nasal-Ginard B, Mahdavi V: Myosin heavy chain messenger RNA and protein isoform transitions during cardiac hypertrophy. J Clin Invest 79: 970–977, 1987
McCully JD, Liew CC: RNA transcription in myocardial cell nuclei during post-natal development. A study establishing an assay system for transcription in vitro. Biochem J 256: 441–445, 1988
Chassagne C, Boheler KR, Schwartz K: Description of an in vitro transcription assay in nuclei isolated from control and hemodynamically overloaded rat cardiac myocytes. CR Acad Sci Paris 312(111): 7–12, 1991
Gunning P, Ponte P, Blau H, Kedes L: α-skeletal and α-cardiac actin genes are coexpressed in adult human skeletal muscle and heart. Mol Cell Biol 3: 1449–1452, 1984
Bennetts BH, Burnett L, Dos Remedios CG: Differential co-expression of α-actin genes with the human heart. J Mol Cell Cardiol 18: 993–996, 1986
Vanderkerckove J, Bugaisky G, Buckingham M: Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. J Biol Chem 261: 1838–1843, 1986
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Boheler, K.R., Carrier, L., Chassagne, C. et al. Regulation of myosin heavy chain and actin isogenes expression during cardiac growth. Mol Cell Biochem 104, 101–107 (1991). https://doi.org/10.1007/BF00229809
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DOI: https://doi.org/10.1007/BF00229809