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The effect of culture and membrane potential on Goα expression in neonatal rat cardiac myocytes

  • Signal Transduction
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Abstract

The effects of culture and membrane potential on Goα39 expression were examined in neonatal rat cardiac myocytes. During six days of culture, the amount of Goα39 in myocytes increased six-fold. The increase in Goα39 appeared to be programmed, since Goα39 of rat hearts also increased in vivo within three days after birth before declining by six days after birth. Furthermore, the age of the rat from which cardiac myocytes were isolated determined the amount of Goα39 that accumulated in cultured cells with myocytes from two day-old rats producing more Goα39 than myocytes from six day-old rats. In addition, agents which alter membrane potential (KCl and bupivacaine) inhibited the accumulation of Goα39 in cultured myocytes. In an attempt to identify the signaling pathway in which cardiac Goα39 is involved, muscarinic receptor-stimulated inositol phosphate production was examined, but was found to be comparable in myocytes that had six-fold differences in Goα39 content. Thus Goα39 does not appear to couple muscarinic receptors to phospholipase C in rat cardiac myocytes.

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Author notes

  1. P. J. McDermott

    Present address: Dept. of Cardiology, Medical University of South Carolina, Veterans Administration Medical Center, 29403, Charleston, SC, USA

Authors and Affiliations

  1. Weis Center for Research, Geisinger Clinic, 17822, Danville, Pennsylvania, USA

    K. A. Foster, P. J. McDermott & J. D. Robishaw

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  2. P. J. McDermott
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  3. J. D. Robishaw
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Foster, K.A., McDermott, P.J. & Robishaw, J.D. The effect of culture and membrane potential on Goα expression in neonatal rat cardiac myocytes. Mol Cell Biochem 104, 63–72 (1991). https://doi.org/10.1007/BF00229805

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