Abstract
Twenty-six days of fat deficiency brought about a decrease of linoleic and an increase of oleic acid in rough endoplasmic reticulum (RER) of guinea pig liver. Arachidonic acid was only slightly decreased in some phospholipids whereas eicose-5,8,11-trienoic acid was not enhanced except in phosphatidyl-inositol. All these changes were relevant specifically in phosphatidylinositol molecules and less important in phosphatidylcholine and phosphatidylethanolamine. Fat deficiency did not modify the relative proportion of phospholipids and cholesterol. Therefore, fat deficient guinea pig microsomes are a good model to study the effect of unsaturated fatty acids on membrane properties. Fluorescent anisotropy of RER membranes, lipids and phospholipids labeled with diphenylhexatriene, was increased by the fat deficiency. The most important increase was observed in liposomes of a mixture of RER phosphatidylinositol, phosphatidylserine and sphingomyelin. A small change was found in phosphatidylcholine and phosphatidylethanolamine dispersions at 37°C. The modification of the lipid unsaturation evoked fluorescent anisotropy changes. Temperature-dependent fluorescent polarization curves of RER membranes labeled with trans-parinaric acid did not show inflections in the temperature range from 5 to 45°C but, RER lipids and phospholipids presented a phase separation at about 20°C. This inflection point was not modified by the fat deficient diet. In those liposomes prepared with a mixture of RER phosphatidylinositol, phosphatidylserine and sphingomyelin, the inflection point was produced at about 37°C.
Similar content being viewed by others
References
Castuma CE, Brenner RR: Biochim Biophys Acta 729:9–16, 1983
Towers NR, Raison JK, Kellerman GM, Linnane AW: Biochim Biophys Acta 287:301–311, 1972
Shinitzky, MM, Barenholz Y: Biochim Biophys Acta 515:367–394, 1978
Andrich MP, Vanderkooi JM: Biochemistry 15:1257–1261, 1976
Van Blitterswijk WJ, Van Hoeven RP, Van der Meer BW: Biochim Biophys Acta 644:323–332, 1981
Sklar LA: Mol Cell Biochem 32:169–177, 1980
Reid ME, Briggs GM: J Nutr 51:341–354, 1953
Blobel G, Potter VR: J Mol Biol 28:539–542, 1967
Ramsey JC, Steele WJ: Anal Biochem 92:305–312, 1979
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: J Biol Chem 193:265–275, 1951
Folch J, Lees M, Sloane-Stanley GH: J Biol Chem 226:497–509, 1957
Huang TC, Chen CP, Wefler V, Raftery J: Anal Chem 33:1405–1406, 1961
Chen PS, Toribara TY, Warner H: Anal Chem 28:1756–1758, 1956
Blobel G, Potter VR: Biochim Biophys Acta 166:48–53, 1968
Fleck A, Munro HN: Biochim Biophys Acta 55:571–583, 1962
Nutter LP, Privett SO: J Chromatog 35:519–525, 1968
Neskovic NM, Kostic OM: J Chromatog 35:297–303, 1968
Azumi T, McGlynn S: J Chem Phys 37:2413–2420, 1962
Chen RF, Bowman RJ: Science 147:729–732, 1965
Sklar LA, Hudson BS, Simoni RD: Biochemistry 16:819–828, 1977
Adelman MR, Blobel G, Sabatini DD: J Cell Biol 56:191–205, 1973
Brenner RR, Garda H, Gomez Dumm INT de, Pezzano H. In: Holman RT (ed) Progress in Lipid Research. Vol 20, 1981, p 315
Gilmore R, Cohn N, Glaser M. Biochemistry 18:1050–1056, 1979
Fraley RT, Yen GSL, Lucking DR, Kaplan S: J Biol Chem 254:1987–1991, 1979
Moore BM, Lentz BL, Meissner G: Biochemistry 17:5248–5255, 1978
Sudipto Das, Rand RP: Biochem Biophys Res Commun 124:491–496, 1984
Sklar LA, Miljanich GP, Dratz EA: Biochemistry 18:1707–1716, 1979
Stubbs CD, Smith AD: Biochim Biophys Acta 779:89–137, 1984
Brenner RR. In: Holman RT (ed) Progress in Lipid Research. Vol 23, 1984, p 69
Jahnig F: Proc Natl Acad Sci 76:6361–6365, 1979
Poon R, Clark WR: Biochim Biophys Acta 649:58–66, 1981
Poon R, Richards JM, Clark WR: Biochim Biophys Acta 689:230–240, 1981
York DA, Hyslop PA, French R: Biochem Biophys Res Commun 106:1478–1483, 1982
Pugh EL, Kates M, Szabo AG: Chem Phys Lipids 30:55–69, 1982
Author information
Authors and Affiliations
Additional information
The author is member of the Carrera del Investigador Cientifico, Consejo Nacional de Investigaciones Cientificas y Técnicas, Argentina.
Rights and permissions
About this article
Cite this article
Soulages, J.L., Brenner, R.R. Effects of fatty acid deficiency on the lipid composition and physical properties of guinea pig rough endoplasmic reticulum. Mol Cell Biochem 78, 109–119 (1987). https://doi.org/10.1007/BF00229685
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00229685