Summary
Antibodies made against thyroglobulin (TG) were used in an immunocytochemical study for the light and electron microscopic localization of TG in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus, during its upstream migration. TG was found in the follicular lumen and in some colloid droplets within the follicular cells. Except for an immunoreactive product observed in a small portion of the interstitial connective tissue, the location of TG in the lamprey was similar to that in the thyroid of the rat.
Similar content being viewed by others
References
Barrington, E.J.W., Sage, M.: The endostyle and thyroid gland. In: The biology of lampreys (Hardisty, M.W. and Potter, I.C., eds.), Vol. 2, pp. 105–134. London: Academic Press 1972
Clausen, J.: Immunochemical techniques for the identification and estimation of macromolecular biology (Work, T.S. and Work, E., eds.), Vol. 1. Amsterdam: North Holland Publishing Co. 1969
Fujita, H., Honma, Y.: Electron microscopical studies on the thyroid of a cyclostome, Lampetra japonka, during its upstream migration. Z. Zellforsch. 73, 559–575 (1966)
Hoheisel, G.: Untersuchungen zur fraktionellen Morphologie des Endostyls und der Thyreoidea vom Bachneunauge (Lampetra planeri Bloch) 2. Untersuchungen an der Thyreoidea. Gegenbaurs morph. Jb. 114, 337–358 (1970)
Lanzing, W.J.R.: Studies on the river lamprey Lampetra fluviatilis during its anadromous migration. Uitgeversmmaatschappi Neerlandia, Utrecht (1959)
Leblond, J., Gross, J.: Thyroglobulin formation in the thyroid follicle visualized by the “coated autoradiograph” technique. Endocrinology 43, 306–324 (1948)
Mollenhauer, H.H.: Plastic embedding mixtures for use in electron microscopy. Stain. Technol. 39, 111–114 (1964)
Mouriz, J., Stanbury, J.B.: Purification of human 19S thyroglobulin by gel filtration. Canad. J. Biochem. 46, 51–58 (1968)
Olivereau, M.: Etude histologique et autoradiographie de la glande thyroide de la lamproie (Petromyzon marinus). Arch. Anat. micr. Morph. exp. 41, 1–10 (1952)
Paiement, J., Leblond, C.P.: Localization of thyroglobulin antigenicity in rat thyroid sections using antibodies labeled with peroxidase and 125I-radioiodine. J. Cell Biol. 74, 992–1015 (1977)
Pantić, V.R.: The cytophysiology of thyroid cells. Int. Rev. Cytol. 38, 153–243 (1974)
Pelletier, G., Puviani, R., Dussault, J.H.: Electron microscope immunohistochemical localization of thyroglobulin in the rat thyroid gland. Endocrinology 98, 1253–1259 (1976)
Peng, T.C., Cooper, C.W., Petrusz, P., Volpert, E.M.: Identification of C-cells in normal and goitrous rat thyroid tissues using antiserum to rat thyrocalcitonin and the immunoperoxidase bridge technique. Endocrinology 97, 1537–1544 (1975)
Shimazaki, M., Ueda, G., Ito, M., Mukobayashi, H., Shirakawa, J.: Staining of the thyroid gland with fluorescein conjugated antithyroglobulin. Wakayama med. Rep. 7, 13–21 (1962)
Sternberger, L.A.: Immunocytochemistry, New Jersey: Prentice Hall 1974
Strum, J.M., Karnovsky, M.J.: Cytochemical localization of endogenous peroxidase in thyroid follicular cells. J. Cell Biol. 44, 655–666 (1970)
Suzuki, S., Gorbman, A., Rolland, M., Montford, M.F., Lissitzky, S.: Thyroglobulin of cyclostomes and an elasmobranch. Gen. comp. Endocr. 26, 59–69 (1975)
Wright, G.M., Youson, J.H.: Transformation of the endostyle of the anadromous sea lamprey, Petromyzon marinus L., during metamorphosis. 1. Light microscopy and autoradiography with 125I. Gen. comp. Endocr. 30, 243–257 (1976)
Author information
Authors and Affiliations
Additional information
Supported by National Research Council of Canada Grant no. A5945 to J.H.Y. We thank Dr. F.W.W. Beamish and Mr. R. Robinson who helped in the capture of the lamprey
Rights and permissions
About this article
Cite this article
Wright, G.M., Filosa, M.F. & Youson, J.H. Light and electron microscopic immunocytochemical localization of thyroglobulin in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus L., during its upstream migration. Cell Tissue Res. 187, 473–478 (1978). https://doi.org/10.1007/BF00229611
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00229611