Abstract
Several studies indicate the presence of hydroxyl radical (OH·) as well as its involvement in the myocardial reperfusion injury. A transition metal-like iron is necessary for the conversion of superoxide anion (O2 −) to a highly reactive and cytotoxic hydroxyl radical (OH·). In the present study, we have examined the generation of OH· and free iron in reperfused hearts following either normothermic (37°C) or hypothermic ischemia (5°C). Employing the Langendorff technique, isolated rat hearts were subjected to global ischemia for 30 min at 37°C or 5°C and were then reperfused for 15 min at 37°C. The results of the study suggest that both the OH· generation in myocardium and free iron release into perfusate were significantly lower in hearts made ischemic at 5°C as compared to 37°C. Release of myoglobin and lactic acid dehydrogenase into perfusate also followed a similar pattern. Furthermore, in in vitro studies, chemically generated O2 − at 5°C caused a significantly lower rate of oxidation of oxymyoglobin as well as generation of OH° and free iron as compared to 37°C. These results suggest that (1) reperfusion of hypothermic ischemic heart is associated with a reduction in the generation of OH· and cellular damage compared to that of normothermic ischemic heart, and (2) myoglobin, an intracellular protein, is a source of free iron and plays a role in the reperfusion injury mediated by free radicals.
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Abbreviations
- OH·:
-
hydroxyl radical
- O2 − :
-
superoxide anion
- ODFR:
-
oxygen-derived free radicals
- KHB:
-
Krebs-Henseleit buffer
- LDH:
-
lactate hydrogenase
- SOD:
-
superoxide dismutase
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Renuka Prasad, M., Liu, X., Rousou, J.A. et al. Reduced free radical generation during reperfusion of hypothermically arrested hearts. Mol Cell Biochem 111, 97–102 (1992). https://doi.org/10.1007/BF00229579
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DOI: https://doi.org/10.1007/BF00229579