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Glycosylation is critical for natriuretic peptide receptor-B function

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Abstract

Co-transfection of a truncated natriuretic peptide receptor-B (NPR-B) with the full length receptor results in a decrease of 60–80% in wild-type receptor activity. This reduction correlates with a loss of glycosylation of the full length NPR-B. This effect is dose-dependent, and occurs with no change in the glycosylation of the truncated receptor. Co-transfection of the full length NPR-B with other receptors yields similar results. These data suggest that glycosylation may be crucial for NPR-B function. Cross-linking studies further demonstrate that only fully glycosylated NPR-B receptors are able to bind ligand. Our data therefore argue that carbohydrate modification may be critical for NPR-B receptor ligand binding.

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Abbreviations

as:

amino acids

ANF:

atrial natriuretic factor

ANOVA:

analysis of variance

BS3 :

bis(sulfosuccinimidyl) suberate

BSA:

bovine serum albumin

CNP:

C-type natriuretic peptide

DEAE:

dextran-diethylaminoethyl-dextran

DMEM:

Dulbecco's modified Eagle medium

DMSO:

dimethyl sulfoxide

dNTP:

deoxynucleotide triphosphate

EDTA:

ethylenediamine tetraacetic acid

IBMX:

3-isobutyl-l-methyl-=xanthine

min:

minutes

N-linked:

asparagine-linked

NPR:

natriuretic peptide receptor

nt:

nucleotide

PCR:

polymerase chain reaction

RIA:

radioimmunoassay

RP-HPLC:

reverse phase-high performance liquid chromatography

RP-HPLC:

reverse phase-high performance liquid chromatography

SDS:

sodium dodecyl sulfate

UV:

ultraviolet

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Authors and Affiliations

  1. Department of Pharmacology, University of Montreal, H3C3J7, Montreal, Quebec, Canada

    Normand McNicoll & André De Léan

  2. Department of Biochemistry, University of Montreal, H3C3J7, Montreal, Quebec, Canada

    Randy Fenrick & André De Léan

Authors
  1. Randy Fenrick
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  2. Normand McNicoll
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  3. André De Léan
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Address for offprints:Department of Pharmacology, University of Montreal, 2900 Edouard Montpetit, Montreal, Quebec, H3C3J7, Canada

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Fenrick, R., McNicoll, N. & De Léan, A. Glycosylation is critical for natriuretic peptide receptor-B function. Mol Cell Biochem 165, 103–109 (1996). https://doi.org/10.1007/BF00229471

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  • DOI: https://doi.org/10.1007/BF00229471

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