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Clathrin-dependent internalization, signaling, and metabolic processing of guanylyl cyclase/natriuretic peptide receptor-A

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Abstract

Cardiac hormones, atrial and brain natriuretic peptides (ANP and BNP), have pivotal roles in renal hemodynamics, neuroendocrine signaling, blood pressure regulation, and cardiovascular homeostasis. Binding of ANP and BNP to the guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) induces rapid internalization and trafficking of the receptor via endolysosomal compartments, with concurrent generation of cGMP. However, the mechanisms of the endocytotic processes of NPRA are not well understood. The present study, using 125I-ANP binding assay and confocal microscopy, examined the function of dynamin in the internalization of NPRA in stably transfected human embryonic kidney-293 (HEK-293) cells. Treatment of recombinant HEK-293 cells with ANP time-dependently accelerated the internalization of receptor from the cell surface to the cell interior. However, the internalization of ligand–receptor complexes of NPRA was drastically decreased by the specific inhibitors of clathrin- and dynamin-dependent receptor internalization, almost 85% by monodansylcadaverine, 80% by chlorpromazine, and 90% by mutant dynamin, which are specific blockers of endocytic vesicle formation. Visualizing the internalization of NPRA and enhanced GFP-tagged NPRA in HEK-293 cells by confocal microscopy demonstrated the formation of endocytic vesicles after 5 min of ANP treatment; this effect was blocked by the inhibitors of clathrin and by mutant dynamin construct. Our results suggest that NPRA undergoes internalization via clathrin-mediated endocytosis as part of its normal itinerary, including trafficking, signaling, and metabolic degradation.

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Acknowledgements

The authors wish to thank Ms. Gevoni Bolden and Ms. Meaghan Bloodworth for their excellent technical help and Mrs. Kamala Pandey for her help in the preparation of the manuscript. We thank Dr. Sandra Schmid for providing a gift of wild-type and mutant dynamin construct. This research was supported by the National Institutes of Health Grants (HL057531 and HL062147).

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  1. Naveen K. Somanna and Indra Mani have contributed equally the first authors.

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  1. Department of Physiology, SL-39, Tulane University Health Sciences Center and School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA

    Naveen K. Somanna, Indra Mani, Satyabha Tripathi & Kailash N. Pandey

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  1. Naveen K. Somanna
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Somanna, N.K., Mani, I., Tripathi, S. et al. Clathrin-dependent internalization, signaling, and metabolic processing of guanylyl cyclase/natriuretic peptide receptor-A. Mol Cell Biochem 441, 135–150 (2018). https://doi.org/10.1007/s11010-017-3180-0

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