Skip to main content
SpringerLink
Log in

Effect of oleate on the apparent Km of monoamine oxidase and the amount of membrane-bound hexokinase in isolated rat hepatocytes: Further evidence for the controlling role of the surface charge in hexokinase binding

  • Original Article
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Brief incubation of isolated rat hepatocytes in the presence of the oleate-bovine serum albumin complex resulted in a release to the cytosol of a portion of hexokinase (EC 2.7.1.1) normally bound to intracellular membranes. This was correlated with an increase of the negative surface potential of the outer mitochondrial membrane, as measured in situ by determining changes of Km of monoamine oxidase (EC 1.4.3.4). It is suggested that non-esterified fatty acids produce a partial release of bound hexokinase in the liver cell by changing the surface charge of intracellular membranes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Klug G, Krause J, Ostlund AK, Knoll G, Brdiczka D: Alterations in liver mitochondrial function as a result of fasting and exhaustive exercise. Biochim Biophys Acta 764:272–282, 1984

    Google Scholar 

  2. Weiler U, Riesinger I, Knoll G, Brdiczka D: The regulation of mitochondrial-bound hexokinase in the liver. Biochem Medicine 33:223–235, 1985

    Google Scholar 

  3. Wojtczak L, Nałęcz MJ: Surface charge of biological membranes as a possible regulator of membrane-bound enzymes. Eur J Biochem 94:99–107, 1979

    Google Scholar 

  4. Famulski KS, Nałęcz MJ, Wojtczak L: Phosphorylation of mitochondrial membrane proteins: Effect of the surface potential on monoamine oxidase. FEBS Lett 157:124–128, 1983

    Google Scholar 

  5. Nałęcz MJ, Wroniszewska A, Famulski KS, Wojtczak L: Changes of the mitochondrial surface potential during cuprizone-induced formation of megamitochondria. Eur J Cell Biol 27:289–295, 1982

    Google Scholar 

  6. Berry MN, Friend DS: High-yield preparation of isolated rat liver parenchymal cells. A biochemical and fine structural study. J Cell Biol 43: 506–520, 1969

    Google Scholar 

  7. Krebs HA, Henseleit K: Untersuchungen über die Harnstoffbildung im Tierkörper. Hoppe-Seyler's Z Physiol Chem 210:33–66, 1932

    Google Scholar 

  8. Bücher Th, Luh W, Pette D: Einfache und zusammengesetzte optische Tests mit Pyridinnucleotiden. In: K Lang and E Lelnartz (eds) Hoppe-Seyler/Thierfelder Handbuch der physiologisch- und pathologisch-chemischen Analyse. Springer-Verlag, Berlin, 1964, vol. 6/A, pp 293–339

    Google Scholar 

  9. Wurtman RJ, Axelrod J: A sensitive and specific assay for the estimation of monoamine oxidase. Biochem Pharmacol 12:1429–1440, 1963

    Google Scholar 

  10. Smith GS, Reid RA: The influence of respiratory state on monoamine oxidase activity in rat liver mitochondria. Biochem J 176:1011–1014, 1978

    Google Scholar 

  11. Scholz R, Schwabe U, Soboll S: Influence of fatty acids on energy metabolism. 1. Stimulation of oxygen consumption, ketogenesis and CO2 production following addition of octanoate and oleate in perfused rat liver. Eur J Biochem 141:223–230, 1984

    Google Scholar 

  12. Goodman DS: The distribution of fatty acids between n-heptane and aqueous phosphate buffer. J Amer Chem Soc 80:3887–3892, 1958

    Google Scholar 

  13. Katz J, Wals PA: Studies with digitonin-treated rat hepatocytes (nude cells). J Cell Biochem 28:207–228, 1985

    Google Scholar 

  14. Adams V, Bosch W, Hämmerle Th, Brdiczka D: Activation of low Km hexokinases in purified hepatocytes by binding to mitochondria. Biochim Biophys Acta, in press

  15. Schnaitman C, Ervin VG, Greenawalt JM: The submitochondrial localization of monoamine oxidase. An enzymatic marker for the outer membrane of liver mitochondria. J Cell Biol 32:719–735, 1967

    Google Scholar 

  16. Wojtczak L, Famulski KS, Nałęcz MJ, Zborowski J: Influence of the surface potential on the Michaelis constant of membrane-bound enzymes: Effect of membrane solubilization. FEBS Lett 139:221–224, 1982

    Google Scholar 

  17. Rodahl K, Miller HI, Issekutz B: Plasma free fatty acids in exercise. J Appl Physiol 19:489–492, 1964

    Google Scholar 

  18. Barakat HA, Kasperek GJ, Dohm GL, Tapscott EB, Snider RD: Fatty acid oxidation by liver and muscle preparations of exhaustively exercised rats. Biochem J 208:419–424, 1982

    Google Scholar 

  19. Issekutz B, Miller HI, Paul P, Rodahl K: Aerobic work capacity and plasma free fatty acid turnover. J Appl Physiol 20:293–296, 1965

    Google Scholar 

  20. Wojtczak L, Nałęcz MJ: The surface potential of membranes: Its effect on membrane-bound enzymes and transport processes. In: Gh Benga (ed) Structure and Properties of Cell Membranes. CRC Press, Boca Raton, Florida 1985, vol. 2, pp 215–242

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Nencki Institute of Experimental Biology, Pasteura 3, 02-093, Warsaw, Poland

    Lech Wojtczak

  2. Faculty of Biology, University of Konstanz, D-7750, Konstanz, Germany

    Volker Adams & Dieter Brdiczka

Authors
  1. Lech Wojtczak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Volker Adams
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dieter Brdiczka
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wojtczak, L., Adams, V. & Brdiczka, D. Effect of oleate on the apparent Km of monoamine oxidase and the amount of membrane-bound hexokinase in isolated rat hepatocytes: Further evidence for the controlling role of the surface charge in hexokinase binding. Mol Cell Biochem 79, 25–30 (1988). https://doi.org/10.1007/BF00229394

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00229394

Key words

Search

Navigation