Abstract
Interspecific hybrids from the crossing Brassica campestris x B. hirta are reported in our study for the first time. F1 plants were obtained by using ovary culture. The phenotype of hybrids was similar to B. napus; the plants were self-fertile. Investigation of meiotic division and nuclear DNA content measurements showed the amphidiploid origin of these hybrids. The relationship between genome A and D, as well as the spontaneous amphidiploidization of the hybrids, are discussed.
Similar content being viewed by others
References
Attia T, Robbelen G (1986) Cytogenetic relationship within cultivated Brassicas analyzed in amphihaploids form the tree diploid ancestors. Can J Genet Cytol 28:323–329
Banga SS (1986) Hybrid pollen-aided induction of matromorphy in Brassica. Z Pflanzenzuecht 96:86–89
Bannerot H, Boulidard L, Cauderon Y, Temple J (1974) Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea. Proc. Eucarpia Meeting Cruciferae, Dundee, Scotland, pp 52–54
Dolezel J, Binarová P, Lucretti S 1989 Analysis of nuclear DNA content in plant cells by flow cytometry. Biol Plant 31(2):113–120
Dyer AF (1963) The use of lactopropionic orcein in rapid squash methods for chromosome preparation. Stain Technol 46:85–90
Eenink AH (1975) Matromorphy in Brassica oleracea L. VII. Research on products of microsporogenesis and gametogenesis from prickle pollinated plants. Euphytica 24:45–52
Halldén C, Bryngelsson T, Sall T, Gustafsson M (1987) Distribution and evolution of tandemly repeated DNA sequence in the family Brassicaceae. In:Tsunoda S, Hinata K, Gommes-Campo C (eds) Brassica crop and wild allies. Japan Scientific Society Press, Tokyo, pp 67–87
Harberd DJ (1975) Cytotaxonomic studies of Brassica and related genera. In: Vaughan JG, Macleod AJ, Jones BMG (eds) The biology and chemistry of the Cruciferae. Academic Press, London New York, pp 47–68
Heyn FW (1977) Analysis of unreduced gametes in the Brassicaceae by crosses between species and ploidy levels. Z Pflanzenzucht 78:13–30
Inomata N (1977) Production of interspecific hybrids between B. campestris and B. oleracea by culture in vitro of excised ovaries. I. Effects of yeast extract and casein hydrolysate on the development of excised ovaries. Jpn J Genet 53:161–173
Inomata N (1985) A revised medium for in vitro culture of Brassica ovaries. In: Chapman GP, Mantell SH, Daniels RW (eds) The experimental manipulation of ovule tissues. Longman, New York London, pp 164–176
Inomata N (1986) Interspecific hybrids between Brassica campestris and B. bourgeami by ovary culture in vitro. Cruciferae Newsl 11:14–15
Inomata N (1990)Interspecific hybridization in Brassica through ovary culture. In: Bajaj YPS (ed) Legumes and oilseed crops I. (Biotechnology in agriculture and forestry, vol 10) Springer, Berlin Heidelberg New York, pp 386–384
Karpechenko GD (1922) The number of chromosomes and the genetic correlation of cultivated Cruciferae. Bull Appl Bot Genet Plant Breed 13:3–14
Matsuzawa Y, Sarashima M (1986) Intergeneric hybridization between Raphanus sativus and Brassica nigra Koch. and alloplasmic derivate. Jpn J Breed 36:122–130
Mizushima U (1946) Some amphihaploids in Cruciferae. Ikushu To Nogei 1:31–32
Mizushima U (1980) Genome analysis in Brassica and allied genera. In: Tsunoda S, Hinata K, Gomes-Campo C (eds) Brassica crops and wild allies. Biology and Breeding. Japan Scientific Society Press, Tokyo, pp 89–108
Mohapatra P, Bajaj YPS (1987) Interspecific hybridization in B. juncea x B. hirta using embryo rescue. Euphytica 36:321–326
Morigana T (1934) On the chromosome number of Brassica juncea and B. napus, on the hybrid between the two and offspring line of the hybrid. Jpn J Genet 94:161–163
Nanda Kumar PBA, Shivanna KR (1986) Interspecific hybridization between B. fruticulosa and B. campestris. Cruciferae Newsl 1:18
Palmer JD, Herbon LA (1987) Unicircular structure of Brassica hirta mitochondrial genome. Curr Genet 11:565–570
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and the origin of aneuploid Brassica species. Tag 65:181–189
Prakash S, Hinata K (1980) Taxonomy, cytogenetics and origin of crop brassicas, a review. Opera Bot 55:1–57
Quiros CF, Ochoa O, Douches DS (1988) Exploring the role of x = 7 species in Brassica evolution: hybridization with B. nigra and B. oleracea. J Hered 79:351–358
Repková J, Smolíková M (1988) Production of F1 interspecific hybrids between B. oleracea and B. campestris through in vitro embryo rescue. Cruciferae Newslett 13:86–87
Ripley VL, Arnisov PG (1990) Hybridization of Sinapis alba L. via embryo rescue. Plant Breeding 104 (1):26–33
Robbelen G (1960) Beiträge zur Analyse des Brassica Genoms. Chromosoma 11:205–228
Robberts IN, Gaude TC, Harrod G, Dickinson HG (1983) Pollenstigma interactions in Brassica oleracea; a new pollen germination medium and its use in elucidating the mechanism of selfincompatibility. Theor Appl Genet 65:231–238
Sarashima M, Matsuzawa Y (1989) Intergeneric hybridization between radish (Raphanus sativus L.) and two monogenomic species of Brassica (B. campestris L. and B. nigra Koch.) Bull Coll Agric Utsonomiya Univ 14 (1):99–104
Shiga T (1980) Male sterility and cytoplasmic differentiation. In: Tsunoda S, Hinata K, Gomez-Campo G (eds) Brassica crops and wild allies. Biology and Breeding Japan Scientific Society Press Tokyo:205–221
UN (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452
Wojciechowski A (1985) Interspecific hybrids between Brassica campestris L. and B. oleracea L. I. Effectiveness of crossing, pollen tube growth embryogenesis. Genet Pol 26 (4):1–14
Zaman MW (1988) Limitation for introgression of yellow seed coat in Brassica napus. Sver Utsaedesfoeren Tidskr 98:157–161
Zenkteler M (1990) In vitro fertilization and wide hybridization in higher plants. Plant Sci 9 (3):267–279
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jandurová, O.M., Dolezel, J. Cytological study of interspecific hybrid between Brassica campestris x B. hirta (Sinapis alba). Sexual Plant Reprod 8, 37–43 (1995). https://doi.org/10.1007/BF00228761
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00228761