Summary
The present study is a detailed kinetic analysis of the synthesis, release and multimerization of fibronectin (FN) in normal and tumor promoter-treated human lung fibroblasts. Pulse/chase and surface labeling experiments were performed to follow the fate of both newly synthesized and pre-existing cell-surface FN over time. The majority of FN (80%) left the intracellular compartment within one hour of synthesis. However, the rate of direct secretion was very low and after one hour, 70% of newly synthesized FN was still at the cell surface. This material was primarily dimeric. Dimeric and multimeric (very high molecular weight) FN was detectable at the cell surface and in the medium 4 hours after synthesis. Pulse-labeled FN multimer levels peaked at 12 hours and declined thereafter. After 24 hours, 85% of pulse-labeled FN had been shed into the medium and the labeled FN remaining at the cell surface was primarily multimeric. Surface labeling experiments confirmed that the majority of FN resides at the cell surface prior to release into the medium.
One hour treatment with the phorbol ester tumor promoter, 12-0-tetradecanoyl phorbol-13-acetate (TPA), stimulated a nine-fold increase in release of preexisting, dimeric cell-surface FN (125I-labeled). The major effect of longer term TPA treatment up to nine hours was continued depletion of dimeric cell-surface FN. Increased release of cell-surface multimeric FN was also stimulated by TPA, but to a much lesser extent. Release of newly synthesized (pulse-labeled) dimeric FN was also stimulated by TPA though much less than pre-existing FN, and TPA treatment produced a small decrease in the steady-state level of multimeric FN. Thus, preexisting cell-surface IN and newly synthesized FN differ dramatically in their susceptibility to TPA treatment.
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Abbreviations
- FN:
-
fibronectin
- HLF:
-
human lung fibroblasts
- PMSF:
-
phenylmethylsulfonylfluoride
- TPA:
-
12-O-tetradecanoyl phorbol-13-acetate
- DMSO:
-
dimethylsulfoxide
- MEM:
-
minimal essential medium
- ELISA:
-
enzyme linked immunosorbent assay
- PBS:
-
phosphate-buffered saline
- BSA:
-
bovine serum albumin
- SDS-PAGE:
-
sodium dodecylsulfate-polyacrylamide gel electrophoresis
- DOC:
-
deoxycholate
- DTT:
-
dithiotreitol
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Burrous, B.A., Wolf, G. The kinetics of fibronectin synthesis and release in normal and tumor promoter-treated human lung fibroblasts. Mol Cell Biochem 96, 57–67 (1990). https://doi.org/10.1007/BF00228453
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DOI: https://doi.org/10.1007/BF00228453