Summary
A Drosophila null mutant(BO-1-4) of α-glycerolphosphate dehydrogenase induced by ethylmethane sulfonate(EMS) was analyzed by double immunodiffusion, enzyme immuno-inactivation, immunoelectrophoresis and two-dimensional electrophoresis. Based on all the immunological evidence, this mutant appears to express no protein that can cross-react with the antiserum specific to α-glycerolphosphate dehydrogenase. A protein spot corresponding to α-glycerolphosphate dehydrogenase was identified on two-dimensional gels of the soluble fly homogenates. The absence of this protein spot on two-dimensional gels of this null mutant further supported the immunological data. The activities of seven other enzymes in the related metabolic pathways were determined for the mutant and the control Drosophila. The null mutant does not show significant alterations in activities of these enzymes. The relationship between the deficiency of this enzyme and the inability for the sustained flight of the null mutant was discussed in terms of cellular metabolic regulations.
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Abbreviations
- α-GPD:
-
α-glycerolphosphate dehydrogenase (EC 1.1.1.8)
- EMS:
-
ethylmethane sulfonate
- TEMED:
-
N,N,N′,N′-tetramethylene diamine
- pI:
-
isolectric point
- CRM:
-
immunological cross-reacting material
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Lee, CY., Read, S. & Johnson, L. Immunological and biochemical analysis of a null mutant of α-glycerolphosphate dehydrogenase from Drosophila melanogaster . Mol Cell Biochem 30, 79–85 (1980). https://doi.org/10.1007/BF00227921
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DOI: https://doi.org/10.1007/BF00227921