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Expression and regulation of chloride channels in neonatal rat cardiomyocytes

  • Part II: (Patho)physiological Responses in Myocardium
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Abstract

Using an 125I− efflux assay, we have studied the expression of various types of chloride channels in isolated neonatal rat cardiomyocytes. Three different classes of anion conductances were distinguished: (1) a Cal2+-sensitive Cl− conductance, triggered upon stimulation of the cells with endothelin-1 or Ca2+-ionophore; (2) a CAMP/protein kinase A-operated Cl− conductance, activated by addition of forskolin. This anion channel could be identified as the Cystic Fibrosis Transmembrane conductance Regulator (CFTR-CI− channel) by Western blotting as well as by its enhanced activity in cultures pretreated with the tyrosine kinase inhibitor genistein; (3) a distinct class of cell volume-regulated Cl− channels, potentiated in the presence of endothelin-1 or the phosphotyrosine phosphatase inhibitor pervanadate. The potential role of each class of Cl− channels in the generation and/or modulation of action potentials as well as in maintaining cell volume is discussed.

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Authors and Affiliations

  1. Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands

    Ben C. Tilly, Karel Bezstarosti, Wendy E.M. Boomaars, Jos M.J. Lamers & Hugo de R.

  2. Department of Veterans Affairs Medical Center, Memphis, Tennessee, USA

    Christopher R. Marino

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  1. Ben C. Tilly
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  2. Karel Bezstarosti
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  3. Wendy E.M. Boomaars
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  4. Christopher R. Marino
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  5. Jos M.J. Lamers
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  6. Hugo de R.
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C. Tilly, B., Bezstarosti, K., E.M. Boomaars, W. et al. Expression and regulation of chloride channels in neonatal rat cardiomyocytes. Mol Cell Biochem 157, 129–135 (1996). https://doi.org/10.1007/BF00227891

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