Summary
As part of continuing multidisciplinary studies on the neuropathogenesis of subacute sclerosing panencephalitis (SSPE), in situ hybridisation, immunocytochemistry and electron microscopy were used to detect measles virus nucleic acid, protein and nucleocapsids in brain perivascular infiltrates of three cases. Perivascular cuffing cells which contained measles virus nucleic acid and antigens were found in all cases. Infected cuffs occurred predominantly in areas of general parenchymal cell infection and in many of these a high proportion of the infiltrating cells were infected. Other cuffs in these areas were either uninfected or contained only a few infected cells. Occasional infected cells were also seen in cuffs in non-infected areas. In contrast, no specific immunocytochemical reactions or in situ hybridisation for measles virus was observed in brain tissue from a patient with herpes encephalitis. By electron microscopy viral nucleocapsid, consistent with measles virus, was found within the cytoplasm of plasma cells in the inflammatory cuffs in SSPE brain tissue. Possible explanations for our results are that infiltrates become infected on arrival in the CNS or alternatively, that the infected infiltrates reflect a generalised infection of the reticuloendothelial system. The frequent presence of uninfected cuffs favours the former explanation.
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Axthelm MK, Krakowka S (1987) Canine distemper virus: the early blood-brain barrier lesion. Acta Neuropathol (Berl) 75:27–33
Brown HR, Goller NL, Rudelli RD, Dymecki J, Wisniewski HM (1989) Postmortem detection of measles virus in nonneural tissues in subacute sclerosing panencephalitis. Ann Neurol 26:263–268
Budka H, Lassmann H, Popow-Kraupp T (1982) Measles virus antigen in panencephalitis. An immunomorphological study stressing dendritic involvement in SSPE. Acta Neuropathol (Berl) 56:52–62
Connolly JH, Allen IV, Hurwitz LJ, Millar JHD (1967) Measles virus antibody and antigen in subacute sclerosing panencephalitis. Lancet I: 542–544
Cosby SL, McQuaid S, Taylor MJ, Bailey M, Rima BK, Martin SJ, Allen IV (1989) Examination of eight cases of multiple sclerosis and 56 neurological and non-neurological controls for genomic sequences of measles virus, canine distemper virus, simian virus 5 and rubella virus. J Gen Virol 70:2027–2036
Esiri MM Oppenheimer DR, Brownell B, Haire M (1981) Distribution of measles antigen and immunoglobulin-containing cells in the CNS in subacute sclerosing panencephalitis (SSPE) and atypical measles encephalitis. J Neurol Sci 53:29–43
Fournier JG, Tardieu M, Lebon P, Robain O, Ponsot G, Rozenblatt S, Bouteille M (1985) Detection of measles virus RNA in lymphocytes from peripheral blood and brain perivascular infiltrates of patients with subacute sclerosing panencephalitis. N Engl J Med 313:910–915
Haase AT, Gantz D, Eble B, Walker D, Stowring L, Ventura P, Blum H, Wietgrefe S, Zupancie M, Tourtellotte WW, Gibbs CJ, Norrby E, Rozenblatt S (1985) Natural history of restricted synthesis and expression of measles virus genes in subacute sclerosing panencephalitis. Proc Natl Acad Sci USA 82:3020–3024
Hall WW, Chopin PW (1981) Measles-virus proteins in the brain tissue of patients with subacute sclerosing panencephalitis. Absence of the M protein. N Engl J Med 304:1152–1155
Joseph BS, Lampert PW, Oldstone MBA (1975) replication and persistence of measles virus in defined subpopulations of human leukocytes. J Virol 16:1638–1649
Kirk J, Zhou A-L, McQuaid S, Cosby SL, Allen IV (1991) Cerebral endothelial cell infection by measles virus in subacute sclerosing panencephalitis: ultrastructural and in situ hybridization evidence. Neuropathol Appl Neurobiol 17:289–297
McQuaid S, Allan GM (1992) Detection protocols for biotinylated probes: optimization using multistep techniques. J Histochem Cytochem 40:569–574
McQuaid S, Isserte S, Allan GM, Taylor MJ, Allen IV, Cosby SL (1990) Use of immunocytochemistry and biotinylated in situ hybridization for detecting measles virus in central nervous system tissue. J Clin Pathol 43:329–333
Moench TR, Griffin DE, Obriecht CR, Vaisberg A, Johnson RT (1988) Acute measles in patients with and without neurologic involvement: distribution of measles virus antigen and RNA. J Infect Dis 158:433–442
Oyanagi S, Rorke LB, Katz M, Koprowski H (1971) Histopathology and electron microscopy of three cases of subacute sclerosing panencephalitis (SSPE). Acta Neuropathol (Berl) 18:58–73
Schneider-Schualies S, Kreth HW, Hoffmann G, Billeter M, ter Meulen V (1991) Expression of measles virus RNA in peripheral blood mononuclear cells of patients with measles, SSPE and autoimmune diseases. Virology 182:703–711
Summers BA, Greisen HA, Appel MJG (1979) Early events in canine distemper demyelinating encephalomyelitis. Acta Neuropathol (Berl) 46:1–10
Ter Meulen V, Stephenson JR, Kreth HW (1983) Subacute sclerosing panencephalitis. In: Fraenkel-Conrat H, Wagner RR (eds) Comprehensive virology, vol 18; Virus host interactions, receptors, persistence and neurological diseases. Plenum Press, New York, pp 105–159
Valdimarsson H, Agnasdottir G, Lachmann PJ (1975) Measles virus receptor on human T lymphocytes. Nature 255:554–556
Wilczynski SP, Cook ML, Stevens JG (1977) Newcastle disease as a model for paramyxovirus-induced neurologic syndromes. Am J Pathol 89:649–666
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McQuaid, S., Kirk, J., Zhou, A.L. et al. Measles virus infection of cells in perivascular infiltrates in the brain in subacute sclerosing panencephalitis: confirmation by non-radioactive in situ hybridization, immunocytochemistry and electron microscopy. Acta Neuropathol 85, 154–158 (1993). https://doi.org/10.1007/BF00227762
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DOI: https://doi.org/10.1007/BF00227762