Abstract
The aim of this study was to evaluate the ability of propionyl-L-carnitine to prevent cardiac damage induced by erucic acid. Rats were fed for 10 days with normal or 10% erucic acid—enriched diets with or without propionyl-L-carnitine intraperitoneally injected, (1 mM/kg daily, for 10 days). The erucic acid diet produced increases in triglycerides (from 5.6 to 12.4 mg/gww, P < 0.01), and free fatty acids (from 2.0 to 5.1 mg/gww, P < 0.01), but no changes in phospholipids. When the hearts were perfused aerobically with an isovolumic preparation there was no difference in mechanical activity. On the contrary, when pressure-volume curves were determined, the pressure developed by hearts from the erucic acid-treated rats were reduced.
Independent of diet, propionyl-L-carnitine treatment always produced positive inotropy. This was concomitant with improved mitochondrial respiration (RCI 5.1 vs 9.3, P < 0.01), higher tissue ATP content (10.3 vs 18.4 μmol/gdw P < 0.01) and reduction of triglycerides (12.4 vs 8.0 mg/gww, P < 0.01). These data suggest that propionyl-L-carnitine, when given chronically, is able to prevent erucic acid-induced cardiotoxicity, probably by reducing triglyceride accumulation and improving energy metabolism.
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References
Litwin B, Sheldon E, Raya TE, Gay RG, Bedotto JB, Bahl JJ, Anderson PG, Goldman S, Bressler R: Chronic inhibition of fatty acid oxidation: new model of diastolic dysfunction. Am J Physiol 258: H51-H56, 1990
Carnitine deficiency. The Lancet 335: 631–632, 1990
Gilbert EF: Carnitine deficiency. Pathol 17: 161–169, 1985
Christophersen BO, Bremer J: Erucic acid-an inhibitor of fatty acid oxidation in the heart. Biochim Biophy Acta 280: 506–514, 1972
Abdellatif AM, Vles RO: Pathological effects of dietary rapeseed oil in rats. Nutr Metab 12(5): 285–295, 1970
Abdellatif AM, Vles RO: Short-term and long-term pathological effects of glyceryl trierucate and of increasing levels of dietary rapeseed oil in rats. Nutr Metab 15(4): 219–231, 1973
Siliprandi N, Di Lisa F, Menabo' R: Propionyl-L-carnitine: biochemical significance and possible role in cardiac metabolism. Cardiovasc Drugs Ther 5: 103–107, 1990
Ferrari R, Pasini E, Condorelli E, Boraso A, Lisciani R, Marzo A: Effect of propionyl-L-carnitine on mechanical function of isolated rabbit heart. Cardiovasc Drugs Ther 3: 403–412, 1990
Bongrani S, Di Lisa F, Raddino R, Ferrari R, Razzetti R, Visioli O: D,L-Carnitine and D,L-acetylcarnitine actions on myocardial contractility and coronary resistance in isolated perfused rabbit hearts. Il Farmaco 5: 1980
Curello S, Ceconi C, Cargnoni A, Medici D, Ferrari R: Oxidative stress during myocardial ischaemia and reperfusion: experimental and clinical evidences. J Appl Cardiol 1: 311–327, 1986
Folch J, Lees M, Stanley GM: A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 226: 497–509, 1957
Sordhal L, McCollum M, Wood W, Schwartz A: Mitochondria and sarcoplasmic reticulum function in cardiac hypertrophy and failure. Am J Physiol 244: 497–502, 1973
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt Biochem 72: 246–254, 1976
Wollenberger A, Ristav O, Schoffa G: Eine einfache technik der extemschellen abkuhlung grosser gewebstuche. Pfluegers Arch 270: 399–402, 1960
Lamprecht W, Trautschold E: Adenosine-5′-triphosphate Determination with Hexokinase and Glucose-6-phosphate Dehydrogenase. In: HU Bergmeryer (ed.) Methods of Enzymatic Analysis. Academic Press, New York 2101–2105
Beare R, Nera EA: Nutritional effects of partially hydrogenated low erucic rapeseed oils. Lipids 12: 769–774, 1977
Ten Floor FM, Van de Graaf HM, Vergrossen AJ: Effects of dietary erucic and linoleic acid on myocardial function in rats. Recent Adv Stud Cardiac Struct Metab 3: 59–72, 1973
Paulson DJ, Traxler J, Schmidt M, et al.: Protection of the ischaemic myocardium by propionyl-L-carnitine: Effects on the recovery of cardiac output after ischaemia and reperfusion, carnitine transport and fatty acid oxidation. Cardiovasc Res 20: 536–541, 1986
Zweier JL, Jacobus WE: Substrate-induced alterations of high energy phosphate metabolism and contractile function in the perfused heart. Biochem Chem 17: 8015–8021, 1987
Ferrari R, Barbato R, Ceconi C, Cerbai E, De Jong JW, Di Lisa F, Menabo' R, Mugelli A, Pasini E: Prolonged propionyl-L-carnitine pretreatment of rabbit: biochemical, hemodynamic and electrophysiological effects on myocardium. J Mol Cell Cardiol. Under consideration.
Ou C, Majumder S, Dai J, Panagia V, Dhalla NS, Ferrari R: Cardiac phosphatidylethanolamine n-methylation in normal and diabetic rats treated with propionyl-i-carnitine. In: Subcellular Basis of contractile failure. Kluwer Academic Publications. Boston 219–234, 1990
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Pasini, E., Cargnoni, A., Condorelli, E. et al. Effect of prolonged treatment with propionyl-L-carnitine on erucic acid-induced myocardial dysfunction in rats. Mol Cell Biochem 112, 117–123 (1992). https://doi.org/10.1007/BF00227568
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DOI: https://doi.org/10.1007/BF00227568