Summary
Protoplasts from pollen grains of Lilium longiflorum regenerate amorphous cellulosic cell walls in culture, during which some precursors of cellulose are polymerized, thus producing progressively harder cellulosic cell walls as the period of culture continues. It is presumed that the components of the cell wall regenerated during 1 week in culture differ from those of the intine of the pollen grain wall. The regenerated cell wall is formed by means of large smooth vesicles; in addition, numerous coated vesicles and pits aid in wall regeneration. The pollen tube that germinates from the 8-day-old cultured protoplast has numerous Golgi bodies and many vesicles which build the pollen tube wall. The tube wall has two layers just like a normal pollen tube wall.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bajaj YSP, Davey MR (1974) The isolation and ultrastructure of pollen protoplasts. In: Lins-kens HF (ed) Fertilization in higher plants. Elsevier, Amsterdam, pp 73–80
Fowke LC, Pickett-Heaps JD (1972) A cytochemical and auto-radiographic investigation of cell wall deposition in fiber cells of Marchantia berteroana. Protoplasma 74:19–32
Fowke LC, Bech-Hansen CW, Gamborg OL, Shyluk JP (1973) Electron microscopic observations of cultured cells and protoplasts of Ammi visnaga. Am J Bot 60:304–312
Fowke LC, Griffing CR, Mersey BG, Van der Valk P (1983) Protoplasts for studies of the plasma membrane and associated cell organelles. (Protoplasts lecture proceedings) Experientia [Suppl] 46:101–110
Griffing LR, Mersey BG, Fowke LC (1985) An investigation of the role of coated vesicles in cell wall regeneration by soybean protoplasts. J Cell Biol 101:49a
Griffmg LR, Mersey BG, Fowke LC (1986) Cell-fractionation analysis of glucan synthase I and II distribution and polysaccharide secretion in soybean protoplasts. Planta 167:175–182
Karnovsky MJ (1971) Use of ferrocyanide-reduced osmium tetroxide in electron microscopy. In: Proceedings of the 11th Annual Meeting of the American Society of Cell Biology, p146
Miki-Hirosige H, Nakamura S (1982) Process of metabolism during pollen tube wall formation. J Electron Microsc 31:51–62
Mizuta S, Sawada K, Okuda K (1985) Cell wall regeneration of new spherical cells developed from the protoplasm of a coenocytotic green alga, Boergesenia forbesii. Jpn J Phycol 33:32–44
Mori K, Matsushima H, Takeuchi M (1983) A study of cell wall regeneration of protoplasts in Marchantia polymorpha L. Bot Mag 96:281–289
Nakamura S, Miki-Hirosige H (1982) Coated vesicles and cell plate formation in the microspore mother cell. J Ultrastruct Res 80:302–311
Pesacreta TC, Lucas WJ (1984) Plasma membrane coat and coated vesicle-associated reticulum of membranes: their structure and possible interrelationship in Chara corallina. J Cell Biol 98:1537–1545
Pojnar E, Willison JHM, Cocking EC (1967) Cell-wall regeneration by isolated tomato-fruit protoplasts. Protoplasma 64:460–480
Reynolds E (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212
Seligman AM, Hanker JS, Wasserkrug H, Dmochowski H, Katzoff L (1965) Histochemical demonstration of some oxidized macromolecules with thoicarbohydrazide (TCH) or thiosemicarbazide (TSC) and osmium tetroxide. J Histochem Cytochem 13:629–639
Simmonds D, Setterfield G, Brown DL (1983) Microtubule organization in plasmolysed plant protoplasts before and during cell-wall regeneration. J Cell Biol 97:207a
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Tanaka I, Kitazume C, Ito M (1987) The isolation and culture of lily pollen protoplasts. Plant Sci 50:205–211
Tanchak MA, Griffing LR, Mersey BG, Fowke LC (1984) Endocytosis of cationized ferritin by coated vesicles of sybean protoplasts. Planta 162:481–486
Van der Valk P, Fowke LC (1981) Ultrastructural aspects of coated vesicles in tobacco protoplasts. Can J Bot 59:1307–1313
Willison JHM, Cocking EC (1972) The production of microfibrils at the surface of isolated tomato-fruit protoplasts. Protoplasma 75:397–403
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Miki-Hirosige, H., Nakamura, S. & Tanaka, I. Ultrastructural research on cell wall regeneration by cultured pollen protoplasts of Lilium longiflorum . Sexual Plant Reprod 1, 36–45 (1988). https://doi.org/10.1007/BF00227021
Issue Date:
DOI: https://doi.org/10.1007/BF00227021