Summary
Attempts were made to produce intergeneric hybrids between Enarthrocarpus lyratus, a wild species, and several species of crop brassicas: B. campestris, B. nigra, B. oleracea, B. juncea, B. napus and B. Carinata. Hybrids using E. lyratus as female parent were realized by means of embryo rescue in four combinations — E. lyratus x B. campestris, E. lyratus x B. oleracea, E. lyratus x B. napus and E. lyratus x B. carinata. Reciprocal crosses showed strong pre-fertilization barriers and yielded no hybrids except in one combination — B. Juncea x E. Lyratus — in which a single hybrid could be realized. All of the hybrids were multiplied in vitro through the multiplication of axillary shoots. Morphological and cytological studies confirmed hybridity. All hybrids were completely pollen sterile except for E. lyratus x B. carinata, which showed 2% pollen fertility. Attempts to double the chromosome number through the in vitro application of colchicine to axillary meristems of F1 hybrids were successful in only one hybrid, E. lyratus x B. oleracea. Cytological studies of the hybrids indicated the presence of a partial homology between the genomes of E. lyratus and crop brassicas. Backcross progenies were raised from all of the five F1 hybrids to develop malesterile alloplasmic lines.
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Agnihotri A, Shivanna KR, Raina SN, Malathi L, Prakash S, Jagannathan V (1990) Production of Brassica napus x Raphanobrassica hybrids by embryo rescue. Plant Breed 105:292–299
Armstrong KC, Keller WA (1981) Chromosome pairing in haploids of B. campestris. Theor Appl Genet 59:49–52
Armstrong KC, Keller WA (1982) Chromosome pairing in haploids of Brassica oleracea. Can J Genet Cytol 24:735–739
Batra V, Shivanna KR, Prakash S (1989) Hybrids of wild species Erucastrum gallicum and crop brassicas. In: Iyama S, Takeda G (eds) Breeding research: the key to the survival of the earth, Proc 6th Int Cong SABRO. The Organizing Committee of the Congress, Tsukuba, pp 443–445
Delourme R, Eber F, Chevre AM (1989) Intergeneric hybridization of Diplotaxis erucoides with Brassica napus. Cytogenetic analysis of F1 and BC1 progeny. Euphytica 41:123–128
Harberd DJ (1976) Cytotaxonomic studies of Brassica and related genera. In: Vaughan JG, Macleod AJ, Jones BMG (eds) The biology and chemistry of the cruciferae. Academic Press, London New York, pp 47–68
Linskens HF, Esser K (1957) Über eine spezifische Anfärbung der Pollenschläuche im Griffel und die Zahl der Kallosepropfen nach Selbstung und Fremdung. Naturwissenschaften 44:16
Matsuzawa Y, Sarashima M (1986) Intergeneric hybridization of Eruca, Brassica and Raphanus. Cruciferae Newsl 11:17
Mizushima U (1980) Genome analysis in Brassica and allied genera. In: Tsunoda SK, Hinata SK, Gomez-Campo C (eds) Brassica crops and wild allies. Jpn Sci Soc Press, Tokyo, pp 89–106
Mohapatra D, Bajaj YPS (1984) In vitro hybridization in an incompatible cross — Brassica juncea x Brassica hirta. Curr Sci 53:489–490
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nanda Kumar PBA, Shivanna KR, Prakash S (1988) Wide hybridization in Brassica. Sex Plant Reprod 1:234–239
Prakash S, Hinata K (1980) Taxonomy, cytogenetics and origin of crop Brassicas, a review. Opera Bot 55:1–57
Ripley VL, Arnison PG (1990) Hybridization of Sinapis alba L and Brassica napus L. via embryo rescue. Plant Breed 104:26–33
Takahata Y (1990) Production of intergeneric hybrids between a C3-C4 intermediate species Moricandia arvensis and a C3 species Brassica oleracea through ovary culture. Euphytica 56:259–264
Takahata Y, Takeda T (1990) Intergeneric (Intersubtribe) hybridization between Moricandia arvensis and Brassica A and B genome species by ovary culture. Theor Appl Genet 80:38–42
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Communicated by H. F. Linskens
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Gundimeda, H.R., Prakash, S. & Shivanna, K.R. Intergeneric hybrids between Enarthrocarpus lyratus, a wild species, and crop brassicas. Theoret. Appl. Genetics 83, 655–662 (1992). https://doi.org/10.1007/BF00226912
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DOI: https://doi.org/10.1007/BF00226912