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Physical maps of Nicotiana chloroplast DNA constructed by an efficient procedure

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Summary

The restriction profiles of chloroplast DNA (cpDNA) from Nicotiana tabacum, N. sylvestris, N. plumbaginifolia, and N. otophora were obtained with respect to AvaI, BamHI, BglI, HindIII, PstI, PvuII, SalI, and XhoI. An efficient mapping method for the construction of cpDNA physical maps in Nicotiana was established via a computer-aided analysis of the complete cpDNA sequence of N. tabacum for probe selection. The efficiency of this approach is demonstrated by the determination of cpDNA maps from N. sylvestris, N. plumbaginifolia, and N. otophora with respect to all of the above restriction endonucleases. The size and basic structure of the cpDNA from the three species are almost identical, with an addition of approximately 80 bp in N. plumbaginifolia. The restriction patterns and hence the physical maps between N. tabacum and N. sylvestris cpDNA are identical and there is no difference in the Pvull digests of cpDNA from all four species. Restriction site variations in cpDNA from different species probably result from point mutations, which create or eliminate a particular cutting site, and they were observed spanning the whole chloroplast molecule but highly concentrated in both ends of the large, single-copy region. The results presented here will be used for the forthcoming characterization of chloroplast genomes in the interspecies somatic hybrids of Nicotiana, and will be of great value in completing the exploration of the phylogenetic relationships within this already extensively studied genus.

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Authors and Affiliations

  1. Institute of Life Science, National Tsing Hua University, Hsinchu, Taiwan 30043, Republic of China

    Ding-I Yang & Yiu-Kay Lai

  2. Institute of Botany, Academic Sinica, Taipei, Taiwan 11529, Republic of China

    Teng-Yung Feng

  3. Department of Botany, National Taiwan University, Taipei, Taiwan 10764, Republic of China

    Chi-Chang Chen

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  1. Ding-I Yang
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  2. Teng-Yung Feng
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  3. Chi-Chang Chen
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  4. Yiu-Kay Lai
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Communicated by Yu. Gleba

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Yang, DI., Feng, TY., Chen, CC. et al. Physical maps of Nicotiana chloroplast DNA constructed by an efficient procedure. Theoret. Appl. Genetics 83, 515–527 (1992). https://doi.org/10.1007/BF00226542

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