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An FMRFamide antiserum differentiates between populations of antigens in the ventral nervous system of the locust, Schistocerca gregaria

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Summary

The distribution of FMRFamide immunoreactive neurones in the ventral nerve cord of the locust, Schistocerca gregaria, is described. These neurones are found only in the suboesophagael and thoracic ganglia, although immunoreactive processes are found in the neuropils of the abdominal ganglia. Many of these neurones also react with an antiserum raised against bovine pancreatic polypeptide (BPP), but this antiserum also reveals another population of cells in the abdominal ganglia. The staining obtained with the BPP antiserum is blocked by preabsorption of the antiserum with FMRFamide; the converse is not true: FMRFamide-like immunoreactivity is not suppressed by preincubation with BPP. These results suggest that there are at least two endogenous peptide antigens in the locust nerve cord: one is found in cells of the suboesophageal and thoracic ganglia, and the other is found in cells of the abdominal ganglia.

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References

  • Bishop CA, O'Shea M (1982) Neuropeptide proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH): Immunocytochemical mapping of neurones in the central nervous system of the cockroach. J Comp Neurol 207:223–238

    Google Scholar 

  • Dockray GJ, Williams RG (1983) FMRFamide-like immunoreactivity in rat brain: development of a radio-immunoassay and its application in studies of distribution and chromatographic properties. Brain Res 266:295–303

    Google Scholar 

  • Dockray GJ, Vaillant C, Williams RG (1981) New vertebrate brain-gut peptide related to a molluscan neuropeptide and an opioid peptide. Nature 293:656–657

    Google Scholar 

  • Dockray GJ, Reeve JR, Shively J, Gayton RJ, Barnard CS (1983) A novel active pentapeptide from chicken brain identified by antibodies to FMRFamide. Nature 305:328–330

    Google Scholar 

  • Duve H, Thorpe A (1982) The distribution of pancreatic polypeptide in the nervous system of the blowfly, Calliphora vomitoria, (Diptera). Cell Tissue Res 227:67–77

    Google Scholar 

  • Greenberg MJ, Price DA (1983) Invertebrate neuropeptides: native and naturalised. Ann Rev Physiol 45:271–288

    Google Scholar 

  • Hansen BL, Hansen GN, Scharrer B (1982) Immunoreactive material resembling vertebrate neuropeptides in the corpus cardiacum and corpus allatum of the insect Leucophaea maderae. Cell Tissue Res 225:319–329

    Google Scholar 

  • Kimmel JR, Pollock HG, Hazelwood RL (1968) Isolation and characterisation of chicken insulin. Endocrinology 83:1323–1330

    Google Scholar 

  • Kimmel JR, Hayden LJ, Pollock HG (1975) Isolation and characterisation of a new pancreatic polypeptide hormone. J Biol Chem 250:9369–9376

    Google Scholar 

  • Lin TM, Chance RE (1972) Spectrum of gastrointestinal actions of a new bovine pancreatic polypeptide (BPP). Gastroenterology 62:852 (Abstract)

    Google Scholar 

  • Moore RY, Gustafson EL, Card JP (1984) Identical immunoreactivity of afferents to the rat suprachiasmatic nucleus with antisera against avian pancreatic polypeptide, molluscan cardioexcitatory peptide and neuropeptide Y. Cell Tissue Res 236:41–46

    Google Scholar 

  • Myers CM, Evans PD (1985) The distribution of bovine pancreatic polypeptide/FMRFamide-like-immunoreactivity in the ventral nervous system of the locust. J Comp Neurol 234:1–16

    Google Scholar 

  • Price DA (1982) The FMRFamide-like peptide of Helix aspersa. Comp Biochem Physiol 72C:325–328

    Google Scholar 

  • Price DA, Greenberg MJ (1977) The structure of a molluscan cardioexcitatory neuropeptide. Science 197:670–671

    Google Scholar 

  • Stern AS, Lewis RV, Kimura S, Rossier J, Gerber LD, Brink L, Stein S, Udenfriend S (1979) Isolation of the opioid heptapeptide Met-enkephalin[Arg6,Phe7] from bovine adrenal medullary granules and striatum. Proc Natl Acad Sci USA 76:6680–6683

    Google Scholar 

  • Sternberger LA (1974) Immunocytochemistry. 2nd Ed. Prentice Hall Inc., New Jersey

    Google Scholar 

  • Tatemoto K (1982a) Isolation and characterisation of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion. Proc Natl Acad Sci USA 79:2514–2518

    Google Scholar 

  • Tatemoto K (1982b) Neuropeptide Y: complete amino acid sequence of the brain peptide. Proc Natl Acad Sci USA 79:5485–5489

    Google Scholar 

  • Tatemoto K, Carlquist M, Mutt V (1982) Neuropeptide Y: a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature (London) 296:659–660

    Google Scholar 

  • Triepel J, Grimmelikhuijzen CJP (1984) A critical examination of the occurrence of FMRFamide immunoreactivity in the brain of guinea pig and rat. Histochemistry 80:63–71

    Google Scholar 

  • Veenstra JA (1984) Immunocytochemical demonstration of a homology in peptidergic neurosecretory cells in the suboesophageal ganglion of a beetle and a locust with antisera to bovine pancreatic polypeptide, FMRFamide, vasopressin and α-MSH. Neuroscience Lett 48:185–190

    Google Scholar 

  • Veenstra JA, Schooneveld H (1984) Immunocytochemical localisation of neurones in the nervous system of the Colorado potato beetle with antisera against FMRFamide and bovine pancreatic polypeptide. Cell Tissue Res 235:303–308

    Google Scholar 

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Myers, C.M., Evans, P.D. An FMRFamide antiserum differentiates between populations of antigens in the ventral nervous system of the locust, Schistocerca gregaria . Cell Tissue Res. 242, 109–114 (1985). https://doi.org/10.1007/BF00225568

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  • DOI: https://doi.org/10.1007/BF00225568

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