Summary
The distribution of FMRFamide immunoreactive neurones in the ventral nerve cord of the locust, Schistocerca gregaria, is described. These neurones are found only in the suboesophagael and thoracic ganglia, although immunoreactive processes are found in the neuropils of the abdominal ganglia. Many of these neurones also react with an antiserum raised against bovine pancreatic polypeptide (BPP), but this antiserum also reveals another population of cells in the abdominal ganglia. The staining obtained with the BPP antiserum is blocked by preabsorption of the antiserum with FMRFamide; the converse is not true: FMRFamide-like immunoreactivity is not suppressed by preincubation with BPP. These results suggest that there are at least two endogenous peptide antigens in the locust nerve cord: one is found in cells of the suboesophageal and thoracic ganglia, and the other is found in cells of the abdominal ganglia.
Similar content being viewed by others
References
Bishop CA, O'Shea M (1982) Neuropeptide proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH): Immunocytochemical mapping of neurones in the central nervous system of the cockroach. J Comp Neurol 207:223–238
Dockray GJ, Williams RG (1983) FMRFamide-like immunoreactivity in rat brain: development of a radio-immunoassay and its application in studies of distribution and chromatographic properties. Brain Res 266:295–303
Dockray GJ, Vaillant C, Williams RG (1981) New vertebrate brain-gut peptide related to a molluscan neuropeptide and an opioid peptide. Nature 293:656–657
Dockray GJ, Reeve JR, Shively J, Gayton RJ, Barnard CS (1983) A novel active pentapeptide from chicken brain identified by antibodies to FMRFamide. Nature 305:328–330
Duve H, Thorpe A (1982) The distribution of pancreatic polypeptide in the nervous system of the blowfly, Calliphora vomitoria, (Diptera). Cell Tissue Res 227:67–77
Greenberg MJ, Price DA (1983) Invertebrate neuropeptides: native and naturalised. Ann Rev Physiol 45:271–288
Hansen BL, Hansen GN, Scharrer B (1982) Immunoreactive material resembling vertebrate neuropeptides in the corpus cardiacum and corpus allatum of the insect Leucophaea maderae. Cell Tissue Res 225:319–329
Kimmel JR, Pollock HG, Hazelwood RL (1968) Isolation and characterisation of chicken insulin. Endocrinology 83:1323–1330
Kimmel JR, Hayden LJ, Pollock HG (1975) Isolation and characterisation of a new pancreatic polypeptide hormone. J Biol Chem 250:9369–9376
Lin TM, Chance RE (1972) Spectrum of gastrointestinal actions of a new bovine pancreatic polypeptide (BPP). Gastroenterology 62:852 (Abstract)
Moore RY, Gustafson EL, Card JP (1984) Identical immunoreactivity of afferents to the rat suprachiasmatic nucleus with antisera against avian pancreatic polypeptide, molluscan cardioexcitatory peptide and neuropeptide Y. Cell Tissue Res 236:41–46
Myers CM, Evans PD (1985) The distribution of bovine pancreatic polypeptide/FMRFamide-like-immunoreactivity in the ventral nervous system of the locust. J Comp Neurol 234:1–16
Price DA (1982) The FMRFamide-like peptide of Helix aspersa. Comp Biochem Physiol 72C:325–328
Price DA, Greenberg MJ (1977) The structure of a molluscan cardioexcitatory neuropeptide. Science 197:670–671
Stern AS, Lewis RV, Kimura S, Rossier J, Gerber LD, Brink L, Stein S, Udenfriend S (1979) Isolation of the opioid heptapeptide Met-enkephalin[Arg6,Phe7] from bovine adrenal medullary granules and striatum. Proc Natl Acad Sci USA 76:6680–6683
Sternberger LA (1974) Immunocytochemistry. 2nd Ed. Prentice Hall Inc., New Jersey
Tatemoto K (1982a) Isolation and characterisation of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion. Proc Natl Acad Sci USA 79:2514–2518
Tatemoto K (1982b) Neuropeptide Y: complete amino acid sequence of the brain peptide. Proc Natl Acad Sci USA 79:5485–5489
Tatemoto K, Carlquist M, Mutt V (1982) Neuropeptide Y: a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature (London) 296:659–660
Triepel J, Grimmelikhuijzen CJP (1984) A critical examination of the occurrence of FMRFamide immunoreactivity in the brain of guinea pig and rat. Histochemistry 80:63–71
Veenstra JA (1984) Immunocytochemical demonstration of a homology in peptidergic neurosecretory cells in the suboesophageal ganglion of a beetle and a locust with antisera to bovine pancreatic polypeptide, FMRFamide, vasopressin and α-MSH. Neuroscience Lett 48:185–190
Veenstra JA, Schooneveld H (1984) Immunocytochemical localisation of neurones in the nervous system of the Colorado potato beetle with antisera against FMRFamide and bovine pancreatic polypeptide. Cell Tissue Res 235:303–308
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Myers, C.M., Evans, P.D. An FMRFamide antiserum differentiates between populations of antigens in the ventral nervous system of the locust, Schistocerca gregaria . Cell Tissue Res. 242, 109–114 (1985). https://doi.org/10.1007/BF00225568
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225568