Summary
In the maturation cycle of the testis of cultured rainbow trout, three periods can be distinguished: a period of spermatogonial proliferation (June–September), a period of maturation, spermiogenesis and spermiation (September–January), and a period of full spermiation (January–June). In the third period new primary spermatogonia are formed, and old sperm cells are resorbed. Sperm release occurs seldom, if ever in animals kept in captivity. The presence of 3β-HSD, 3α-HSD and G6PD activities, smooth endoplasmic reticulum and mitochondria with tubular cristae indicates steroidogenesis in Leydig cells. These parameters have been found during the annual cycle; the strongest steroidogenic activity is from January to June, when metabolic activities tend to shift to the pentose phosphate shunt. In June, most steroid synthesizing Leydig cells are abundant, at sites where seminiferous tubules contain primary and secondary spermatogonia. In November, a weak steroidogenic activity has also been demonstrated in Sertoli cells lining post-spermatogonial cysts. Other aggregations of steroid synthesizing cells are present outside the testis in the connective tissue surrounding the epithelium of vas deferens. The epithelium of the vas deferens shows positive reaction for 3α-HSD, G6PD, MD, LD and NADHD throughout the testicular cycle; 3α-HSD and G6PD activities are generally weak. Metabolic activity in this epithelium increases when sperm cells are stored in the lumen of the duct (November–June). Acid phosphatase in the epithelium of the vas deferens is more active during the period when sperm cells are resorbed.
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References
Ahsan, S.N.: Cyclical changes in the testicular activity of the lake chub, Couesius plumbeus (Agassiz). Canad. J. Zool. 44, 149–171 (1966)
Baillie, A.H., Calman, K.C., Ferguson, M.M., Mck. Hart, D.: Developments in steroid histochemistry. London and New York: Academic Press, 1966
Barka, T., Anderson, P.: Histochemical method for acid phosphatase using hexazonium pararosaline as coupling. J. Histochem. Cytochem. 10, 741–753 (1963)
Billard, R., Jalabert, B., Breton, B.: Les cellules de Sertoli des poissons téléostéens. I. Etude ultrastructurale. Ann. Biol. anim. Biochem. Biophys. 12, 19–32 (1972)
Boddingius, J.: The cell types of the adenohypophysis in the rainbow trout (Salmo irideus). A histological study. Thesis. Oxford: Holywell Press Ltd, 1975
Chayen, J., Bitenski, L., Butcher, R., Poulter, L.: Histochemistry, a guide to practical histochemistry. Edinburgh: Oliver, Boyd, 1969
Christensen, A.K.: Leydig cells. In: Handbook of physiology. Sect. 7, Vol. V. Washington: Am. Physiol. Soc. 1975
Drance, M.G., Hollenberg, M.J., Smith, M., Wylie, V.: Histological changes in trout testis produced by injections of salmon pituitary gonadotropin. Canad. J. Zool. 54, 1285–1293 (1976)
Foley, J.O.: The spermatogenesis of Umbra limi with special reference to the behaviour of the spermatogonial chromosomes and the first maturation division. Biol. Bull. 50, 117–147 (1926)
Geiser, S.W.: Seasonal changes in the testes of Gambusia affinis, the top minnow. 19th Annual meeting Amer. Soc. Zool. 32–33 (1921)
Gresik, E.W.: Homologs of Leydig and Sertoli cells in the testis of the teleost Oryzias latipes. In: Electron microscopic concepts of secretion. Ultrastructure of endocrine and reproductive organs (M. Hess, ed.), Chap. 14, pp. 231–242. New York: Wiley & Sons, Inc. 1975
Henderson, N.E.: The annual cycle in the testis of the eastern brook trout, Salvelinus fontinalis (Mitchell). Canad. J. Zool. 40, 631–645 (1962)
Henderson, N.E.: The urinary and genital systems of trout. J. Fish. Res. Bd. Can. 24, 447–449 (1967)
Hiroi, O., Yamamoto, K.: Studies on the maturation of Salmonid fishes. II. Changes in the testis of the masu salmon, Oncorhynchus masou, during anadromous migration. Bull. Fac. Fish. Hokkaido Univ. 20, 252–271 (1970)
Hurk, R. van den: The localization of steroidogenesis in the testis of oviparous and viviparous teleosts. Proc. kon. ned. Akad. Wet., Ser. C 76, 270–279 (1973)
Hurk, R. van den: Morphological and functional aspects of the testis of the black molly (Mollienisia latipinna). Thesis, University of Utrecht (1975)
Hurk, R. van den, Barends, P.: Cytochemistry of the intratesticular efferent duct system of the black molly (Mollienisia latipinna). Proc. kon. ned. Akad. Wet., Ser. C 77, 205–214 (1974)
Hurk, R. van den, Kant, H. van de: The effect of methyltestosterone, 11-ketotestosterone and methallibure on gonadotropic cells, Leydig cells, spermatogenesis and the epithelium of the intratesticular efferent duct system of the juvenile black molly (Mollienisia latipinna). Proc. kon. ned. Akad. Wet., Ser. C 78, 275–286 (1975)
Hurk, R. van den, Meek, J., Peute, J.: Ultrastructural study of the testis of the black molly (Mollienisia latipinna). I. The intratesticular efferent duct system. Proc. kon. ned. Akad. Wet., Ser. C 77, 460–469 (1974)
Hurk, R. van den, Meek, J., Peute, J.: Ultrastructural study of the testis of the black molly (Mollienisia latipinna). II. Sertoli cells and Leydig cells. Proc. kon. ned. Akad. Wet., Ser. C 77, 470–476 (1974)
Hurk, R. van den, Testerink, G.J.: The effect of methallibure and methyltestosterone on gonadotropic cells, Leydig cells and the intratesticular efferent duct system of the adult black molly (Mollienisia latipinna). Proc. kon. ned. Akad. Wet., Ser. C 78, 265–274 (1975)
Jones, J.W.: Histological changes in the testis in the sexual cycle of male salmon parr (Salmo salar L. juv.). Proc. roy. Soc. B, 128, 499–509 (1940)
Lagios, M.D.: Seasonal changes in the cytology of the adenohypophysis, testes and ovaries of the black seaperch, Embiotoca jacksoni, a viviparous percomorph fish. Gen. comp. Endocr. 5, 207–221 (1965)
Lofts, B., Bern, H.A.: The functional morphology of steroidogenic tissues. In: Steroids in nonmammalian vertebrates. New York and London: Academic Press 1972
Lofts, B., Marshall, A.J.: Cyclical changes in the distribution of the testis lipids of a teleost fish, Esox lucius. Quart. J. micr. Sci. 98, 74–88 (1957)
Marshall, A.J., Lofts, B.: The Leydig cell homologue in certain teleost fishes. Nature (Lond.) 177, 704–705 (1956)
Nicholls, T.J., Graham, G.P.: The ultrastructure of lobule boundary cells and Leydig cell homologs in the testis of a cichlid fish, Cichlasoma nigrofasciatum. Gen. comp. Endocr. 19, 133–146 (1972)
O'Halloran, M.J., Idler, D.R.: Identification and distribution of the Leydig cell homologue in the testis of sexually mature atlantic salmon (Salmo salar). Gen. comp. Endocr. 15, 361–364 (1970)
Oordt, P.G.W.J. van, Brands, F.: The Sertoli cells in the testis of the common frog, Rana temporaria. J. Endocr. 48, abstract (1970)
Oota, J., Yamamoto, K.: Interstitial cells in the immature testes of rainbow trout. Ann. Zool. Jap. 39, 142–148 (1966)
Oota, J., Yamamoto, K., Takano, K., Sakaguchi, T.: Studies in the maturing process of the rainbow trout, Salmo gairdnerii irideus. II. Maturation of the testis of a one year old fish. Bull. Jap. Soc. Sci. Fish. 31, 597–603 (1965)
Pearse, A.G.E.: Histochemistry theoretical and applied. London: Churchill Ltd (1961)
Peute, J., Bruyn, M.G.A. de, Seldenrijk, R., Oordt, P.G.W.J. van: Cytophysiology and innervation of gonadotropic cells in the pituitary of the black molly (Poecilia latipinna). Cell Tiss. Res. 174, 35–54 (1976)
Rastogi, R.K.: Seasonal variations in the gonads of certain Indian teleostean fishes. Thesis. Vikram University (1966)
Remacle, C.: Actions hormonales sur les cellules germinales mâles de Carassius auratus L. en culture organotypique. Gen. Comp. Endocr. 29, 480–491 (1976)
Robertson, O.H.: Accelerated development of testis after unilateral gonadectomy, with observations on normal testis of rainbow trout. Bull. Fish. Wildlife Serv. 58, 9–30 (1958)
Rudolph, G., Klein, H.J.: Histochemische Darstellung und Verteilung der Glucose-6-Phosphatdehydrogenase in normalen Rattenorganen. Histochem. 4, 238–251 (1964)
Saksena, D.N.: Reproductive organs and the reproductive cycle of male Indian freshwater goby, Glossogobius giuris (Ham.). Zool. Jb. Anat. 95, 127–146 (1976)
Savard, K., Marsh, J.M., Howell, D.S.: Progesterone biosynthesis in luteal tissue. Role of nicotinamide adenine dinucleotide phosphate and NADP-linked dehydrogenases. Endocrinol. 73, 554–563 (1963)
Schreck, C.B., Flickinger, S.A., Hopwood, M.L.: Plasma androgen levels in intact and castrate rainbow trout, Salmo gairdneri. Proc. Soc. Symp. Biol. Med. 140, 1009–1011 (1972)
Turner, C.L.: The seasonal cycle in the spermary of the perch. J. Morph. 32, 681–711 (1919)
Weisel, G.F.: A histological study of the testes of the sockeye salmon (Oncorhynchus nerka). J. Morph. 73, 207–230 (1943)
Yamazaki, F., Donaldson, E.M.: Involvement of gonadotropin and steroid hormones in the spermiation of the goldfish (Carassius auratus). Gen. comp. Endocr. 12, 491–497 (1969)
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The authors wish to thank Prof. Dr. P.G.W.J. van Oordt for critically reviewing the manuscript. Thanks are also due to Mr. G.J. Schuttebelt for delivering the trout, to Mr. J. Stegenga and Mr. F. Welter for technical assistance and to the members of the light optical registration department
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van den Hurk, R., Peute, J. & Vermeij, J.A.J. Morphological and enzyme cytochemical aspects of the testis and vas deferens of the rainbow trout, Salmo gairdneri . Cell Tissue Res. 186, 309–325 (1978). https://doi.org/10.1007/BF00225540
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DOI: https://doi.org/10.1007/BF00225540