Summary
Castrated adult male and female and androgenized female rats (AS rats) were injected i.v. with 3H-estradiol (E2). Nuclear uptake and retention of the 3H-steroid was examined in pituitary luteinizing hormone (LH) and prolactin (PRL) cells by the combined techniques of autoradiography and immunocytochemistry. About 80% of PRL cells were found to concentrate the radioactive steroid compound in all experimental groups, while 89%, 82% and 68% of LH cells were found to be labeled in AS rats, normal female and male rats, respectively. This suggests that there are subpopulations of LH or PRL cells that contain no or, if any, small numbers of E2 receptor. Statistical analysis revealed that PRL cells take up more radioactivity than LH cells in male rats, while there is no significant difference between female and AS rats. Variations in E2 uptake (coefficient of variation) was higher in LH cells than in PRL cells in male rats and in AS rats. In females, on the contrary, coefficient of variation was larger in PRL cells. Thus the characteristics of nuclear uptake and retention of estradiol in LH and PRL cells appear to be modulated in part by neonatal androgen since the pattern found in AS rats is different than that found in normal male and female rats.
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References
Bogdanove EM, Spirtos BN, Halmi NS (1955) Further observations on pituitary structure and function in rats bearing hypothalamic lesions. Endocrinology 57:302–315
Gorski RA (1971) Gonadal hormones and perinatal development of neuroendocrine function. In: Martini L, Ganong WF (eds) Frontiers in neuroendocrinology. Oxford Press, New York, 237–390
Gustafsson JA (1970) Steroids in germfree and conventional rats. Steroid monosulfate in urine from female rats. Eur J Biochem 14:560–568
Herbert DC, Sheridan PJ (1983) Uptake and retention of sex steroids by the baboon pituitary gland-evidence of sexual dimorphism with respect to dihydrotestosterone. Biol Reprod 28:377–383
Herbert DC, Sheridan PJ (1984) Identification of the progesterone target cells in the female baboon pituitary gland. Biol Reprod 30:479–483
Herbert DC, Weaker FJ, Sheridan PJ (1981) Localization of 3H-dihydrotestostrone in the pituitary gland of the rhesus monkey. Cell Tissue Res 215:499–505
Herbert DC, Weaker FJ, Sheridan PJ (1982) Identification of target cells for estrogen in the pars distalis of the armadillo by immunocytochemistry. Gen Comp endocrinol 47:94–103
Ison EJ, Sheridan PJ (1981) Autoradiography of diffusible substances — a practical approach. Am J Med Technol 47:38–42
Kao LWL, Weisz J (1975) Direct effect of testosterone and its 5α-reduced metabolites on pituitary LH and FSH release in vitro. Change in pituitary responsiveness to hypothalamic extract. Endocrinology 96:253–260
Keefer DA (1980) In vitro estrogen uptake by individual cell types of the rat anterior pituitary after short term castration and adrenalectomy. Cell Tissue Res 209:167–175
Keefer DA (1981a) Quantification of in vivo 3H-estrogen uptake by individual anterior pituitary cell types of the male rat: a combined autoradiographic-immunocytochemical technique. J Histochem Cytochem 29:167–174
Keefer DA (1981b) Induction by progesterone of a sexual dimorphism of estrogen uptake by anterior pituitary cells in situ. Cell Tissue Res 215:75–86
Keefer DA, Stumpf WE, Petrusz P, Sar M (1975) Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary. Am J Anat 142:129–135
Keefer DA, Stumpf WE, Petrusz P (1976) Quantitative autoradiographic assessment of 3H-estradiol uptake in immunocytochemically characterized pituitary cells. Cell Tissue Res 166:25–35
Lieberman ME, Maurer RA, Gorski J (1978) Estrogen control of prolactin synthesis in vitro. Proc Natl Acad Sci 75:5946–5949
Lieberman ME, Maurer RA, Claude P, Gorski J (1982) Prolactin synthesis in primary cultures of pituitary cells: regulation by estradiol. Mol Cell Endocrinol 25:277–294
Mallampati RS, Johnson DC (1973) Serum and pituitary luteinizing hormone, follicle stimulating hormone and prolactin levels in gonadectomized male, female and androgenized female rats treated with estradiol benzoate. J Endocrinol 59:209–216
Maurer RA (1982) Relationship between estradiol, ergocriptine and thyroid hormone: effects on prolactin synthesis and prolactin messenger ribonucleic acid levels. Endocrinology 110:1515–1520
Nilson LH, Nejedlik MT, Virgin JB, Crowder ME, Nett TM (1983) Expression of α subunit and luteinizing hormone β genes in the ovine anterior pituitary. J Biol Chem 258:12087–12090
Nogami H (1984) Fine structural heterogeneity and morphologic changes in rat pituitary prolactin cells after estrogen and testosterone treatment. Cell Tissue Res 227:195–202
Nogami H, Yoshimura F (1982) Fine structural criteria of prolactin cells identified immunohistochemically in the male rat. Anat Rec 202:261–274
Sar M, Stumpf WE (1979) Simultaneous localization of steroid and peptide hormones in rat pituitary by combined thaw-mount autoradiography and immunocytochemistry: localization of dihydrotestosterone in gonadotrophs and pituicytes. Cell Tissue Res 203:1–7
Sar M, Stumpf WE (1981) Combined autoradiography and immunocytochemistry for simultaneous localization of radioactively labeled steroid hormones and antibodies in the brain. J Histochem Cytochem 29:161–166
Stone RT, Maurer RA, Gorski J (1977) Effect of estradiol-17β on preprolactin messenger ribonucleic acid activity in the rat pituitary gland. Biochemistry 16:4915–4921
Stumpf WE (1968) Cellular and subcellular 3H-estradiol localization in the pituitary by autoradiography. Z Zellforsch 92:23–33
Stumpf WE, Sar M (1975) Autoradiographic techniques for localizing steroid hormones. Methods Enzymol 36:135–156
Warembourg M (1977) Radioautographic localization of estrogenconcentrating cells in the brain and pituitary of the guinea pig. Brain Res 123:357–362
Yamamoto K, Taylor LM, Cole FE (1970) Synthesis and release of GH and prolactin from the rat anterior pituitary in vitro as function of age and sex. Endocrinology 87:21–26
Yamamoto K, Kasai K, Ieiri T (1975) Control of pituitary functions of synthesis and release of prolactin and growth hormone by gonadal steroids in female and male rats. Jpn J Physiol 25:645–658
Yoshimura F, Nogami H, Shirasawa N, Yashiro T (1981) A whole range of fine structural criteria of immunohistochemically identifiable LH cells in rats. Cell Tissue Res 217:1–10
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Nogami, H., Yoshimura, F., Herbert, D.C. et al. Does neonatal androgen modulate uptake and retention of 3H-estradiol in gonadotrophs and lactotrophs?. Cell Tissue Res. 242, 629–632 (1985). https://doi.org/10.1007/BF00225429
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DOI: https://doi.org/10.1007/BF00225429