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Intramural distribution of immunoreactive vasoactive intestinal polypeptide (VIP), substance P, somatostatin and mammalian bombesin in the oesophago-gastro-pyloric region of the human gut

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Summary

The intramural distribution of vasoactive intestinal polypeptide (VIP), substance P, somatostatin and mammalian bombesin was studied in the oesophago-gastro-pyloric region of the human gut. At each of 21 sampling sites encompassing this entire area, the gut wall was separated into mucosa, submucosa and muscularis externa, and extracted for radioimmunoassay. VIP levels in the mucosa were very high in the proximal oesophagus (1231±174 pmol/g, mean±SEM) and showed varied, but generally decreasing concentrations towards the stomach, followed by a clear-cut increase across the pyloric canal (distal antrum: 73±16 pmol/g, proximal duodenum: 366±62 pmol/ g); consistent levels were found in submucosa and muscle (200–400 pmol/g) at most sites, the stomach again showing lower concentrations. By contrast, substance P was present in small amounts as far as the proximal stomach, but sharply increased across the pyloric canal, especially in mucosa and submucosa (distal antrum: 20±6.5 and 5.5±1.3 pmol/g; proximal duodenum: 62±8.5 and 34±11 pmol/g, respectively). Somatostatin concentrations were very low in the mucosa of the oesophagus and stepwise increased in the cardiac, mid-gastric and pyloric mucosa (cardia: 224±72 pmol/g; distal antrum: 513±152 pmol/g; proximal duodenum: 1013±113 pmol/g); concentrations in the submucosa and muscularis were generally low, with the exception of antrum and duodenum. Mammalian bombesin was comparatively well represented throughout the oesophageal muscularis (5–8 pmol/g), but most abundant in the stomach in all layers (oxyntic mucosa: 24±2.7 pmol/g; submucosa: 20±5.7 pmol/g; muscle: 28±5.0 pmol/g). In conclusion, a distinct differential distribution of the four peptides studied was revealed, indicating a diffuse, but highly differentiated peptide-containing innervation of the proximal human gut.

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Authors and Affiliations

  1. Department of Anatomy, "Tor Vergata" University, Roma, Italy

    Gian-Luca Ferri, Leonardo Soimero & Daniela Cavalli

  2. Departments of Medicine, RPMS, Hammersmith Hospital, London, UK

    Thomas E. Adrian, Marion Blank & Stephen R. Bloom

  3. Departments of Histochemistry, RPMS, Hammersmith Hospital, London, UK

    Julia M. Polak

  4. Department of Surgery, University of Firenze, Firenze, Italy

    Giancarlo Biliotti

Authors
  1. Gian-Luca Ferri
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  2. Thomas E. Adrian
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  3. Leonardo Soimero
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  4. Marion Blank
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  5. Daniela Cavalli
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  6. Giancarlo Biliotti
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  7. Julia M. Polak
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  8. Stephen R. Bloom
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Ferri, GL., Adrian, T.E., Soimero, L. et al. Intramural distribution of immunoreactive vasoactive intestinal polypeptide (VIP), substance P, somatostatin and mammalian bombesin in the oesophago-gastro-pyloric region of the human gut. Cell Tissue Res. 256, 191–197 (1989). https://doi.org/10.1007/BF00224734

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  • DOI: https://doi.org/10.1007/BF00224734

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