Summary
The influence of long (light: dark 16∶8) and short (light:dark 8∶16) photoperiods on the autonomic innervation of the ductus epididymidis and the ductus deferens of Phodopus sungorus was studied using electron microscopy with morphometric analyses, and biochemical methods. At short photoperiods, only the large smooth muscle cells in the ductus deferens became atrophic, the number of mainly adrenergic varicosities in the smooth muscle layer decreased, and the mean distance between varicosity and smooth muscle cells increased. The content of noradrenaline was determined by high-performance liquid chromatography with electrochemical detection. For the ductus deferens, the noradrenaline content was reduced at LD 8∶16 to less than 10% of the initial value. Short photoperiods are proposed to influence only the adrenergic innervation of the large smooth muscle cells of the ductus deferens. These cells are believed to exert a trophic influence on their nerves.
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References
Alm P (1982) On the autonomic innervation of the human vas deferens. Brain Res Bull 9:673–677
Baumgarten HG, Holstein AF, Rosengren E (1971) Arrangement, ultrastructure, and adrenergic innervation of smooth musculature of the ductuli efferentes, ductus epididymidis and ductus deferens of man. Z Zellforsch 120:37–79
Bergmann M (1987) Photoperiod and testicular function in Phodopus sungorus. Adv Anat Embryol Cell Biol, Vol 105
Bergmann M, Leetz C, Schindelmeiser J, Kindermann EM, Kutzner M, Hoffmann K (1987) Photoperiodically induced changes in the epididymis of the Djungarian hamster, Phodopus sungorus. Acta Endocrinol (Kbh) 114, Suppl 283:179–180
Burnstock G (1986a) The changing face of autonomic neurotransmission. Acta Physiol Scand 126:67–91
Burnstock G (1986b) Autonomic neuromuscular junctions: current developments and future directions. J Anat 146:1–30
Campbell G, Haller CJ, Rogers DC (1978) Fine structural and cytochemical study of the innervation of smooth muscle in an amphibian (Bufo marinus) lung before and after denervation. Cell Tissue Res 194:419–432
Feher E, Burnstock G (1987) Ultrastructural identification of vasoactive intestinal polypeptide- and neuropeptide Y-containing nerve fibres in the vas deferens of the guinea-pig. J Autonom Nerv Syst 19:235–242
Fried G, Lagercrantz H, Hökfelt T (1978) Improved isolation of small noradrenergic vesicles from rat seminal ducts following castration. A density gradient centrifugation and morphological study. Neuroscience 3:1271–1291
Greenberg J, Schubert W, Metz J, Yanaihara N, Forssmann WG (1985) Studies of the guinea-pig epididymis. III. Innervation of epididymal segments. Cell Tissue Res 239:395–404
Gundert-Remy U, Hildebrandt R, Stiehl A, Zürcher G, DaPrada M (1983) Intestinal absorption of levodopa in man. Eur J Clin Pharmacol 25:69–72
Hoerster M, Hoffmann K (1987) Endocrine changes after prolonged exposure to short photoperiods in the Djungarian hamster, Phodopus sungorus. Proc First Eur Congr Endocrinol (Kbh) Abstr No 18-528
Hoffmann K (1981) Photoperiodism in Vertebrates. In: Aschoff J (ed) Handbook Behav Physiol, Vol IV. Raven Press, New York, pp 449–473
Kilts DK, Breese GR, Mailman RB (1981) Simultaneous quantification of dopamine, 5-hydroxytryptamine and four metabolically related compounds by means of reversed-phase high-performance liquid chromatography with electrochemical detection. J Chromatogr 225:347–357
Kvist U, Sjöstrand NO (1976) A further contribution to the question of trophic and hormonal influences on the noradrenaline content of the male reproductive tract: effect of combined androgen and estrogen treatment of prepubertally castrated rats. Acta Physiol Scand 98:339–346
Risley PL, Skrepetos CN (1964) Cholinesterase distributions in the rat epididymis and vas deferens after castration and sex hormone treatments. Anat Rec 150:195–208
Ryd G, Sjöstrand NO (1967) Effect of castration and testosterone administration on the noradrenaline content of the vas deferens and the seminal vesicle of the guinea-pig. Experientia 23:816–817
Schindelmeiser J, Kutzner M, Kindermann EM, Wittkowski W, Bergmann M, Hoffmann K (1987) Photoperiodically induced changes of the morphology and innervation of the ductus deferens of Phodopus sungorus. Acta Endocrinol (Kbh) 114, Suppl 283:130–131
Schindelmeiser J, Kutzner M, Bergmann M, Aumüller G, Heck H, Hoffmann K (1988a) Influence of long and short photoperiods on the morphology and androgen receptor levels of the epididymis and the ductus deferens of Phodopus sungorus. Andrologia (in press)
Schindelmeiser J, Kindermann EM, Hoffmann K (1988b) Photoperiodic influence on the innervation of the ductus epididymidis and ductus deferens of Phodopus sungorus — light microscopic studies. Anat Anz (in press)
Sjöstrand NO (1975) Critical comment on the ‘trophic’ influence of the effector organ on the sympathetic nerves of the male sex accessories of the guinea-pig. Experientia 31:1428–1429
Sjöstrand NO (1981) Smooth muscles of vas deferens and other organs in the male reproductive tract. In: Bülbring E, Brading AF, Jones AW, Tomita T (eds) Edward Arnold, London, pp 367–376
Sjöstrand NO, Swedin G (1976) Influence of age, growth, castration and testosterone treatment on the noradrenaline levels of the ductus deferens and the auxiliary male reproductive glands of the rat. Acta Physiol Scand 98:323–338
Tranzer JP, Richards JG (1976) Ultrastructural cytochemistry of biogenic amines in nervous tissue: methodological improvements. J Histochem Cytochem 24:1178–1193
Wakade AR, Kirpekar SM (1973) “Trophic” influence on the sympathetic nerves of the vas deferens and seminal vesicle of the guinea pig. J Pharmacol Exp Ther 186:528–536
Wakade AR, Garcia AG, Kirpekar SM (1975) Effect of castration on the smooth muscle cells of the internal sex organs of the rat: influence of the smooth muscle on the sympathetic neurons innervating the vas deferens, seminal vesicle and coagulating gland. J Pharmacol Exp Ther 193:424–434
Weber E (1980) Grundriß der biologischen Statistik. 8. Aufl. G. Fischer Verlag, Stuttgart
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Schindelmeiser, J., Kutzner, M., Rolf, L.H. et al. Photoperiodic influence on the innervation of the ductus epididymidis and ductus deferens of the Djungarian hamster, Phodopus sungorus: electron-microscopic and biochemical results. Cell Tissue Res. 256, 175–181 (1989). https://doi.org/10.1007/BF00224732
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DOI: https://doi.org/10.1007/BF00224732