Summary
The adrenergic innervation of the pulmonary vasculature of the file snake Acrochordus granulatus was examined by use of glyoxylic acid-induced fluorescence. Perivascular plexuses of blue-green fluorescent nerves are observed around the common pulmonary artery, the anterior and posterior pulmonary arteries, the arterioles leading to the gas exchange capillaries of the lung, the venules draining the lung, and the anterior and posterior pulmonary veins. Adrenergic nerves are also associated with the visceral smooth muscle of the lung septa and other tissues. Thus, adrenergic control of pulmonary blood flow may occur either at the common pulmonary artery or more regionally within the lung. Regional control of blood flow in the elongate lung of this snake may be important in matching pulmonary perfusion with the distribution of respiratory gas. Glyoxylic acid-histochemistry and immunohistochemistry revealed that populations of cells located in the common pulmonary artery contain the indoleamine 5-hydroxy-tryptamine. Many of the cells are intimately associated with varicose blue-green fluorescent nerves. It is proposed that the 5-hydroxytryptamine-containing cells may be involved in intravascular chemoreception.
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References
Benchetrit G, Armand J, Dejours P (1977) Ventilatory chemoreflex drive in the tortoise, Testudo horsfieldi. Resp Physiol 31:183–191
Berger PJ (1973) Autonomic innervation of the visceral and vascular smooth muscle of the lizard lung. Comp Gen Pharmacol 4:1–10
Berger PJ, Burnstock G (1979) Autonomic nervous system. In: Gans C (ed) Biology of the Reptilia, vol 5. Academic Press, London New York, pp 1–57
Berger PJ, Gibbins IL, Hards DK, Crosby LJ (1982) The distribution and ultrastructure of sensory elements in the baroreceptor region of the truncus arteriosus of the lizard Trachydosaurus mgosus. Cell Tissue Res 226:389–406
Burggren WW (1977) The pulmonary circulation of the chelonian reptile: morphology, haemodynamics and pharmacology. J Comp Physiol 116:303–323
Burggren WW (1985) Hemodynamics and regulation of central cardiovascular shunts in reptiles. In: Johansen K, Burggren WW (eds) Cardiovascular shunts, Alfred Benzon Symposium 21. Munskgaard, Copenhagen, pp 121–142
Burggren WW (1987) Form and function in reptilian circulations. AmZool 27:5–19
Coupland RE, Fujita T (1976) Chromaffin, enterochromaffin and related cells. Elsevier, Amsterdam
Eyzaguirre C, Zapata P (1984) Perspectives in carotid body research. J Appl Physiol: Resp Env Exercise Physiol 57:931–957
Fujita T (1981) Paraneuron, its current implication. In: Kanno T (ed) Paraneurons, their features and functions. Excerpta Medica, Amsterdam, pp 3–9
Furness JB, Costa M (1975) The use of glyoxylic acid for the fluorescence histochemical demonstration of peripheral stores of noradrenaline and 5-hydroxytryptamine in whole mounts. Histochemistry 41:335–352
Furness JB, Moore J (1970) The adrenergic innervation of the cardiovascular system of the lizard, Trachysaurus rugosus. Z Zellforsch 108:150–176
Goniakowska-Witalinska L (1980) Endocrine-like cells in the lungs of the newt, Triturus alpestris Laur. Cell Tissue Res 210:521–524
Goniakowska-Witalinska L (1981) Neuroepithelial bodies in the lung of the tree frog Hyla arborea L. A scanning and transmission electron microscopic study. Cell Tissue Res 217:435–441
Jones DR, Milsom WK (1982) Peripheral receptors affecting breathing and cardiovascular function in non-mammalian vertebrates. J Exp Biol 100:59–91
Jonsson G (1971) Quantitation of fluorescence biogenic mono-amines demonstrated with the formaldehyde fluorescence method. Prog Histochem Cytochem 2:299–334
Kobayashi S (1971) Comparative cytological studies of the carotid body. 1. Demonstration of monoamine storing cells by correlated chromaffin reaction and fluorescence histochemistry. Arch HistolJpn 33:319–339
Lauweryns JM, Cokelaere M, Theunynck P (1972) Neuro-epithelial bodies in the respiratory mucosa of various mammals. A light optical, histochemical and ultrastructural investigation. Z Zellforsch 135:569–592
Lillywhite HB, Donald JA (1987) Neurovascular structure and function in the elongate lung of an aquatic snake. Physiologist 30:190
Lillywhite HB, Smits AW (1985) Diving adaptations in the aquatic file snake, Acrochordus granulatus. Physiologist 28:283
McLean JR, Burnstock O (1967) Innervation of the lungs of the sleepy lizard (Trachysaurus rugosus). 1. Fluorescent histochemistry of catecholamines. Comp Biochem Physiol 22:809–813
McLelland J, Mcfarlane CJ (1986) Solitary granular endocrine cells and neuroepithelial bodies in the lungs of the ringed turtle dove (Streptopelia risoria). J Anat 147:83–93
Milsom WK, Langille BL, Jones DR (1977) Vagal control of pulmonary vascular resistance in the turtle Chrysemys scripta. Can J Zool 55:359–367
Rogers DC, Haller CJ (1978) Innervation and cytochemistry of the neuroepithelial bodies in the ciliated epithelium of the toad lung (Bufo marinus). Cell Tissue Res 195:395–410
Scheuermann DW, De Groodt-Lassel MHA, Stilman C, Meisters ML (1983) A correlative light-, fluorescence- and electron microscopic study of the neuroepithelial bodies in the lung of the red-eared turtle Pseudemys scripta elegans. Cell Tissue Res 234:249–269
Smith DG, MacIntyre DH (1979) Autonomic innervation of the visceral and vascular smooth muscle of a snake lung (Ophidae: Colubridae). Comp Biochem Physiol 62C: 187–191
Smith RV, Satchell DG (1987) Histochemistry of the lung of the Australian snake-necked tortoise, Chelodonia longicollis. J Morphol 192:257–268
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Donald, J.A., Lillywhite, H.B. Adrenergic nerves and 5-hydroxytryptamine-containing cells in the pulmonary vasculature of the aquatic file snake Acrochordus granulatus . Cell Tissue Res. 256, 113–118 (1989). https://doi.org/10.1007/BF00224724
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DOI: https://doi.org/10.1007/BF00224724