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Morphology of cultured guinea-pig cerebellum

I. Pattern of development. Comparison of phase contrast cinematography and silver impregnations of various cell types

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Summary

Guinea-pig cerebellum has been cultured in three in vitro systems: Maximow slides, Leighton tubes and Rose chambers. The best results were obtained from foetuses at the 34th day of gestation. Myelination appeared at the 21st day in vitro and progressed until after one month in culture. It was observed in 62% of the cultures on Maximow slides or in Leighton tubes. Myelination was quite variable in Rose chambers. The organization of the cultures was quite similar to that previously described in other species. Most types of neurons and glia were identified by phase contrast microscopy and microcinematographic analysis. Various histological stains and impregnations accompanied studies of living cells. Despite the lack of specificity of metal impregnations when applied to tissue cultures, successive study of similar or identical fields by phase contrast and such methods has facilitated cellular identification and demonstrated the limits of each of the techniques used. The difficulty of cell identification in the outgrowth zone is emphasized. Nevertheless, the following cell types have been identified:

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  • neurons: external granule cells, internal granule cells, Purkinje cells, roof nucleus type I and II neurons,

  • glial cells: immature cells, oligodendrocytes, astrocytes, histiocytic cells.

The guinea-pig cerebellum is characterized in vitro as it is in vivo by a particularly slow development when compared to other mammals.

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References

  • Allerand, C. D.: Patterns of neuronal differentiation in developing cultures of neonatal mouse cerebellum: a living and silver impregnation study. J. comp. Neurol. 142, 167–204 (1971)

    Article  CAS  PubMed  Google Scholar 

  • Allerand, C. D., Murray, M. R.: Myelin formation in vitro. Endogeneous influences on cultures of newborn mouse cerebellum. Arch. Neurol. (Chic.) 19, 292–301 (1968)

    Article  CAS  Google Scholar 

  • Altman, J., Gopal-Das, D.: Postnatal neurogenesis in the guinea-pig. Nature (Lond.) 214, 1098–1101 (1967)

    Article  CAS  Google Scholar 

  • Bornstein, M. B.: Reconstituted rat-tail collagen used as substrate for tissue cultures on coverslips in Maximov slides and roller tubes. Lab. Invest. 7, 134–137 (1958)

    CAS  PubMed  Google Scholar 

  • Bornstein, M. B., Murray, M. R.: Serial observations on patterns of growth, myelin formation, maintenance and degeneration in cultures of newborn rat and kitten cerebellum. J. Biophys. biochem. Cytol. 4, 499–504 (1958)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Boutry, J. M.: Etude comparative, histologique et dynamique des cellules de la névroglie du cervelet de cobaye en culture. Mémoire Diplôme Ecole Pratique des Hautes Etudes, Paris (1972)

  • Canti, R. G., Bland, J. O.W., Russell, D. S.: Tissue culture of gliomata. Ass. Res. nerv. Dis. Proc. 16, 1 (1935)

    Google Scholar 

  • Dobbing, J., Sands, J.,: Growth and development of the brain and spinal cord of the guinea pig. Brain Res. 17, 115–123 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Hauw, J.-J.: Aspects récents du tissu nerveux en culture. I — Méthodes actuelles de culture. Presse méd. 77, 939–942 (1969)

    CAS  PubMed  Google Scholar 

  • Hauw, J.-J., Goube de Laforest, P., Anteunis, A., Cathala, F., Robineaux, R.: Culture organotypique prolongée de tissu nerveux en chambre perfusable. C. R. Acad. Sci. (Paris) 269, 1205–1208 (1969)

    CAS  Google Scholar 

  • Hauw, J.-J., Novikoff, A. B., Novikoff, P. M., Boutry, J. M., Robineaux, R.: Culture of nervous tissue on collagen in Leighton tubes. Brain Res. 27, 301–309 (1972)

    Article  Google Scholar 

  • Hendelmann, W. J., Booher, J.: Factors involved in the culturing of chick embryo dorsal root ganglia in the Rose chamber. Tex. Rep. Biol. Med. 24, 83–90 (1966)

    Google Scholar 

  • Hild, W.: Observations on neurons and neuroglia from the area of mesencephalic fifth nucleus of the cat “in vitro”. Z. Zellforsch. 47, 127–146 (1957)

    Article  CAS  PubMed  Google Scholar 

  • Hild, W.: Cell types and neuronal connections in cultures of mammalian central nervous tissue. Z. Zellforsch. 69, 155–188 (1966)

    Article  CAS  PubMed  Google Scholar 

  • Ingebrigtsen, R.: Regeneration of axis cylinders “in vitro”. J. exp. Med. 18, 412–415 (1913)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kim, S. U.: Observations on cerebellar granule cells in tissue culture. A silver and electron microscopic study. Z. Zellforsch. 107, 454–465 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Lapham, L. W., Markesbery, W. R.: Human fetal cerebellar cortex: Organization and maturation of cells “in vitro”. Science 173, 829–832 (1971)

    Article  CAS  PubMed  Google Scholar 

  • Lasher, R. S., Zagon, I. S.: The effect of potassium on neuronal differentiation in cultures of dissociated newborn rat cerebellum. Brain Res. 41, 482–488 (1972)

    Article  CAS  PubMed  Google Scholar 

  • Lebar, R., Boutry, J. M., Robineaux, R., Voisin, G. A.: Induction et prévention de l'encéphalite allergique expérimentale chez le cobaye. Relation avec les lésions induites “in vitro” et avec la classe et la spécificité des auto-anticorps produits. C. R. Acad. Sci. (Paris) 276, 1635–1638 (1973)

    CAS  Google Scholar 

  • Lumsden, C. E.: Tissue culture in relation to tumours of the nervous system. In: Pathology of tumours of the nervous system (D. S. Russel, L. J. Rubinstein, eds.), p. 287–331. London: Arnolds 1963

    Google Scholar 

  • Lumsden, C. E.: Nervous tissue in culture. In: The structure and function of nervous tissue (G. H. Bourne, ed.), vol. 1, p. 68–140. New York-London: Academic Press 1968

    Google Scholar 

  • Murray, M. R.: Nervous tissues “in vitro”. In: Cells and tissues in culture. (E. N. Willmer, ed.) vol. II, p. 373–455. London-New York: Academic Press 1965

    Google Scholar 

  • Nakai, J.: Transformation and multiplication of neuroglia in tissue culture. In: IV International Congress of neuropathology proceedings, vol. II, p. 241–246. Stuttgart: Thieme 1962

    Google Scholar 

  • Nakai, J., Okamoto, M.: Identification of neuroglial cells in tissue culture. In: Morphology of neuroglia (J. Nakai, ed.). Tokyo: Igaku Shoin 1963

    Google Scholar 

  • Penfield, W., Cone, W. V.: Neuroglia and microglia. The metallic methods. In: Handbook of microscopical technique (McClung Jones, ed.), 3rd ed., p. 399–431. New York: Paul B. Koeber Inc. 1950

    Google Scholar 

  • Peterson, E. R., Bornstein, H. B., Crain, S. M.: Development, myelination and function in cultures of mammalian spinal cord. Excerpta med. (Amst.) (Sect. I), 16, 813 (1962)

    Google Scholar 

  • Pomerat, C. M.: Pulsatile activity of cells from the human brain in tissue culture. J. nerv. ment. Dis. 114, 430–449 (1951)

    CAS  PubMed  Google Scholar 

  • Pomerat, C. M.: Dynamic neuropathology. J. Neuropath. exp. Neurol. 14, 28–38 (1955)

    Article  CAS  PubMed  Google Scholar 

  • Pomerat, C. M., Costero, I.: Normal and neoplastic brain in time-lapse cinematography. In: Proceedings second international congress of neuropathology, p. 629–634. London: The Excerpta Medica Foundation 1955

    Google Scholar 

  • Pomerat, C. M., Costero, I.: Tissue culture of cat cerebellum. Amer. J. Anat. 99, 211–247 (1956)

    Article  PubMed  Google Scholar 

  • Raine, C. S.: Ultrastructural applications of cultured nervous systems tissue to neuropathology. In: Progress in neuropathology (H. M. Zimmerman, ed.) vol. 2, p. 27–68. New York and London: Grune & Stratton 1973

    Google Scholar 

  • Raine, C. S., Bornstein, M. B.: Experimental allergic encephalomyelitis: an ultrastructural study of experimental demyelination “in vitro”. J. Neuropath. exp. Neurol. 29, 177–191 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Ramón y Cajal, S.: Histologie du système nerveux de l'homme et des vertébrés, vol. 2. Paris: Maloine 1911

    Google Scholar 

  • Rio Hortega, P. del, Penfield, W.: Cerebral cicatrix, reaction of neuroglia and microglia to brain wounds. Johns Hopk. Hosp. Bull. 41, 278 (1927)

    Google Scholar 

  • Robineaux, R., Moncel, C.: Installation 16 m/m pour l'analyse image par image d'enregistrements microcinématographiques. Res. Film 6, 472–476 (1969)

    Google Scholar 

  • Robineaux, R., Pinet, J., Kourilsky, R.: Etude microcinématographique de la rate en culture sous membrane de dialyse. Nouv. Rev. franc. Hémat. 2, 797–811 (1962)

    CAS  PubMed  Google Scholar 

  • Seil, F. J.: Neuronal groups and fiber patterns in cerebellar tissue cultures. Brain Res. 42, 33–51 (1972)

    Article  CAS  PubMed  Google Scholar 

  • Seil, F. J., Herndon, R. M.: Cerebellar granule cells in vitro. A light and electron microscope study. J. Cell Biol. 45, 212–220 (1970)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shein, H. M.: Propagation of human fetal spongioblasts and astrocytes in dispersed cell cultures. Exp. Cell Res. 40, 554–569 (1965)

    Article  CAS  PubMed  Google Scholar 

  • Shein, H. M.: Neoplastic transformation of hamster astrocytes and choroid plexus cell in culture by polyoma virus. J. Neuropath. exp. Neurol. 29, 70–88 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Storts, R. W., Koestner, A.: General cultural characteristics of canine cerebellar explants. Amer. J. vet. Res. 29, 2351–2364 (1968)

    CAS  PubMed  Google Scholar 

  • Suyeoka, O., Okamoto, M.: Granule cells of the mouse cerebellum cultured “in vitro”. Their identification, degeneration and perikaryal myelin. Arch. histol. jap. 27, 117–130 (1966)

    Article  Google Scholar 

  • Wolf, M. K.: Anatomy of cultured mouse cerebellum. II. Organotypic migration of granule cells demonstrated by silver impregnation of normal and mutant cultures. J. comp. Neurol. 140, 281–298 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Wolf, M. K., Dubois-Dalcq, M.: Anatomy of cultured mouse cerebellum. I. Golgi and electron microscopic demonstrations of granule cells, their afferent and efferent synapses. J. comp. Neurol. 140, 261–280 (1970)

    Article  CAS  PubMed  Google Scholar 

  • Wolf, M. K., Holden, A. B., Harlan, P. M.: The fate of granule cell precursors in myelinating cultures of mouse cerebellum. Anat. Rec. 157, 343 (1967)

    Google Scholar 

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Authors and Affiliations

  1. Centre de Physiologie et d'Immunologie Cellulaires INSERM U. 104, CNRS et Association Claude Bernard, Hôpital Saint-Antoine, 12, Paris Cedex, France

    Jean-Jacques Hauw, Jeanne-Marie Boutry, Nadine Crosnier-Suttin & Roger Robineaux

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  1. Jean-Jacques Hauw
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  2. Jeanne-Marie Boutry
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  3. Nadine Crosnier-Suttin
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  4. Roger Robineaux
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Hauw, JJ., Boutry, JM., Crosnier-Suttin, N. et al. Morphology of cultured guinea-pig cerebellum. Cell Tissue Res. 152, 141–164 (1974). https://doi.org/10.1007/BF00224691

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