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Observations on cerebellar granule cells in tissue culture

A silver and electron microscopic study

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Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

In long-term organized cultures of newborn mouse cerebellum, granule cell neurons were studied with silver impregnation and electron microscopy.

In silver impregnated cultures, small neurons are defined as granule cell neurons from their size, morphology and location. There are also occasional large nerve endings with the morphology typical of mossy fiber endings.

In correlative electron micrographs, granule cell neurons revealed a faithful reproduction of characteristic structures seen in vivo. The fine structural details of cerebellar glomeruli and myelinated granule cell bodies developed in vitro were also described.

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Authors and Affiliations

  1. Department of Anatomy, College of Medicine, University of Saskatchewan, Saskatoon, Canada

    Seung U. Kim

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  1. Seung U. Kim
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Additional information

This work was initiated while the author was a fellow of National Multiple Sclerosis Society in the laboratory of Dr. Margaret R. Murray at the Department of Surgery, Columbia University, New York, U.S.A. This work was supported by the Medical Research Council of Canada.

The author is greatly indebted to Dr. Asao Hirano, Laboratory Division, Montefiore Medical Center, New York, U.S.A., for his contribution in the preparation of certain electronmicrographs. The author wishes to thank Dr. S. Fedoroff for reading the manuscript.

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Kim, S.U. Observations on cerebellar granule cells in tissue culture. Z. Zellforsch. 107, 454–465 (1970). https://doi.org/10.1007/BF00335434

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  • DOI: https://doi.org/10.1007/BF00335434

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