Summary
The supraoptic (SON) and paraventricular (PVN) nuclei of the lizard Liolaemus cyanogaster c. were studied by use of histochemical, immunocyto-chemical and electron microscopic methods. The immunofluorescence staining for neurophysin was applied to methacrylate-embedded material before and after treatment of the sections with urea and trypsin. Pseudoisocyanine was applied to sections previously used for immunocytochemistry. The ultrastructural study showed that the SON and PVN neurons possess neurosecretory granules (nsg), distributed throughout the perikaryon, and large (2 to 12 μm) electron-dense droplets located within dilatations of the cisternae of the rough endoplasmic reticulum. Whereas the perikaryon (nsg) and the secretory droplets are stainable with pseudoisocyanine, only the former displays immunoreactivity for neurophysin. However, after treating the sections with urea and trypsin, the same secretory droplets become immunoreactive. It is suggested that the secretory droplets are sites of storage for the precursor of neurophysin, and that the tryptic digestion either triggers its conversion into neurophysin or exposes its immunoreactive sites. Based on the ultrastructure and the histochemical behavior of the secretory droplets, it is also postulated that they contain, in addition to peptides, a glycoprotein component.
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References
Bargmann W (1949) Über die neurosekretorische Verknüpfung von Hypothalamus und Neurohypophyse. Z Zellforsch 34:610–634
Bergman LW, Kuehl WM (1977) Addition of glucosamine and mannose to nascent immunoglobulin heavy chains. Biochemistry 16:4490–4497
Castel M, Hochman J (1976) Ultrastructural immunohistochemical localization of vasopressin in the hypothalamic-neurohypophysial system of three murids. Cell Tissue Res 174:69–82
Dreifuss JJ (1975) A review on neurosecretory granules: their contents and mechanisms of release. Ann NY Acad 248:184–201
Gabe M (1953) Sur quelques applications de la coloration par la fuchsine-paraldehyde. Bull Microsc Appl 3:153–162
Gainer H, Brownstein MJ (1978) Identification of the precursors of the rat neurophysins. In: Vincent JD, Kordon C (eds) Biologie cellulaire des processus neurosécrétoires hypothalamiques. Edit Centre Nat Rech Sci, Paris, pp 525–541
Hew Ch-L, Yin CC (1976) Biosynthesis of polypeptide hormones. Can J Biochem 54:591–599
Kiely ML, McKnight GS, Schimke RT (1976) Studies on the attachment of carbohydrate to ovalbumin nascent chains in an oviduct. J Biol Chem 251:5490–5495
Krisch B (1978) Light- and electron microscopic localization of vasopressin or a vasopressin-like substance in the neurons of the rat suprachiasmatic nucleus. Cell Tissue Res 194:361–365
Morris JF (1978) Structural aspects of hormone production and storage in neurosecretion. In: Vincent JD, Kordon C (eds) Biologie cellulaire des processus neurosécrétoires hypothalamiques. Edit Centre Nat Rech Sci, Paris, pp 601–618
Murakami M (1963) Weitere Untersuchungen über die Feinstruktur der neurosekretorischen Zellen im Nucleus supraopticus von Gecko japonicus. Z Zellforsch 59:684–699
Murakami M (1964) Elektronenmikroskopische Untersuchungen am Nucleus praeopticus der Kröte (Bufo vulgaris formosus). Z Zellforsch 63:208–225
Oksche A, Farner DS, Serventy DL, Wolff F, Nicholls CA (1963) The hypothalamo-hypophysial neurosecretory system of the Zebra Finch, Taeniopygia castanotis. Z Zellforsch 58:846–914
Pearse AGE (1960) Histochemistry. J A Churchill Ltd, London
Pickering BT (1978) The neurosecretory neurone: A model system for the study of secretion. Ess Biochem 14:45–81
Richardson KC, Jarett L, Finke, EH (1960) Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol 35:313–323
Rodríguez EM (1968) Morphology and biological activities of the choroid plexus and some regions of the ventricular walls. PhD Thesis, Bristol
Rodríguez EM (1969) Fixation of the central nervous system by perfusion of the cerebral ventricles with a threefold aldehyde mixture. Brain Res 15:395–412
Rodríguez EM (1979 in preparation) Methacrylate embedding as a useful tool in histochemistry and immunocytochemistry
Scharrer E (1928) Die Lichtempfindlichkeit blinder Elritzen. I. Untersuchungen über das Zwischenhirn der Fische. Z Vergl Physiol 7:1–38
Scharrer E (1951) Neurosecretion. X. A relationship between the paraphysis and the paraventricular nucleus in the garter snake (Thamnophis sp.). Biol Bull 101:106–113
Scharrer E, Scharrer B (1954) Neurosekretion. In: Möllendorff W v, Bargmann W (eds) Handb d mikr Anat d Menschen. Springer, Berlin Göttingen Heidelberg, Vol 6/V, pp 953–1066
Silverman AJ (1976) Ultrastructural studies on the localization of neurohypophysial hormones and their carrier proteins. J Histochem Cytochem 24:816–827
Silverman AJ, Zimmerman EA (1975) Ultrastructural immunocytochemical localization of neurophysin and vasopressin in the median eminence and posterior pituitary. Cell Tissue Res 159:291–301
Sterba G, Weiss J (1967) Beiträge zur Hydrencephalokrinie: I. Hypothalamische Hydrencephalokrinie der Bachforelle (Salmo trutta fario). J Hirnforsch 9:359–371
Tager HS, Steiner DF (1974) Peptide hormones. Ann Rev Biochem 43:509–538
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Supported by Grant S-77-28 from the Dirección de Investigaciones, Universidad Austral de Chile
The authors wish to thank Prof. B.T. Pickering for providing the antineurophysin serum and Mrs. Elizabeth Santibáñez for her assistance
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González, C.B., Rodríguez, E.M. Ultrastructure and immunocytochemistry of neurons in the supraoptic and paraventricular nuclei of the lizard Liolaemus cyanogaster . Cell Tissue Res. 207, 463–477 (1980). https://doi.org/10.1007/BF00224620
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DOI: https://doi.org/10.1007/BF00224620