Skip to main content
SpringerLink
Log in

Vitronectin and proliferative intraocular disorders. II. Expression of cell surface receptors for fibronectin and vitronectin in periretinal membranes

  • Published:
International Ophthalmology Aims and scope Submit manuscript

Abstract

Several cell types participate in the formation of vitreoretinal traction membranes in proliferative intraocular disorders. The communication between these cells involves hormones, growth factors, and the interaction with extracellular matrix molecules. We have previously demonstrated a partial colocalisation of two potent mediators of cell attachment, fibronectin and vitronectin, in periretinal membranes from patients with proliferative vitreoretinopathy (PVR). We found a similar pattern of vitronectin and fibronectin deposition in proliferative diabetic retinopathy (PDR) (n = 6). Now we show the expression of the corresponding cell surface receptors, integrins, for fibronectin and vitronectin by proliferating cells in 22 periretinal membranes, including traumatic (n = 8) and idiopathic (n = 8) PVR as well as PDR membranes (n = 6). Integrins are membrane receptors for extracellular matrix macromolecules which are involved in such basic biological phenomena as embryogenesis and metastasis. Future studies on the pathogenesis of vitreoretinal proliferation will have to focus on the initiation, maintenance, and regulation of this intercellular communication network involving attachment proteins and integrins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akiyama SK, Yamada KM. Fibronectin. Adv Enzymol 1987; 59: 1–57.

    CAS  PubMed  Google Scholar 

  • Argraves WS, Suzuki S, Arai H, Thompson K, Pierschbacher MD, Ruoslahti E. Amino acid sequence of the human fibronectin receptor. J Cell Biol 1987; 105: 1183–1190.

    Article  CAS  PubMed  Google Scholar 

  • Avery RL, Glaser BM. Inhibition of retinal pigment epithelial cell attachment by a synthetic peptide derived from the cell-binding domain of fibronectin. Arch Ophthalmol 1986; 104: 1220–1222.

    Article  CAS  PubMed  Google Scholar 

  • Brown EJ, Goodwin JL. Fibronectin receptors of phagocytes. Characterization of the Arg-Gly-Asp binding proteins of human monocytes and polymorphonuclear leukocytes. J Exp Med 1988; 167: 777–793.

    Article  CAS  PubMed  Google Scholar 

  • Buck CA, Horwitz AF. Cell surface receptors for extracellular matrix molecules. Annu Rev Cell Biol 1987; 3: 179–205.

    Article  CAS  PubMed  Google Scholar 

  • Burns GF, Lucas CM, Krissansen GW, Werkmeister JA, Scanlon DB, Simpson RJ, Vadas MA. Synergism between membrane gangliosides and Arg-Gly-Asp-directed glycoprotein receptors in attachment to matrix proteins by melanoma cells. J Cell Biol 1988; 107: 1225–1230.

    Article  CAS  PubMed  Google Scholar 

  • Campochiaro PA, Jerdan JA, Glaser BM. Serum contains chemoattractants for human retinal pigment epithelial cells. Arch Ophthalmol 1984; 102: 1830–1833.

    Article  CAS  PubMed  Google Scholar 

  • Campochiaro PA, Jerdan JA, Glaser BM, Cardin A, Michels RG. Vitreous aspirates from patients with proliferative vitreoretinopathy stimulate retinal pigment epithelial cell migration. Arch Ophthalmol 1985; 103: 1403–1405.

    Article  CAS  PubMed  Google Scholar 

  • Collins WE, Mosher DF, Tomasini BR, Cooper SL. A preliminary comparison of the thrombogenic activity of vitronectin and other RGD-containing proteins when bound to surfaces. Ann NY Acad Sci 1987; 516: 291–299.

    Article  CAS  PubMed  Google Scholar 

  • Dedhar S, Ruoslahti E, Pierschbacher MD. A cell surface receptor complex for collagen type I recognizes the arg-gly-asp sequence. J Cell Biol 1987; 104: 585–593.

    Article  CAS  PubMed  Google Scholar 

  • Dejana E, Colella S, Conforti G, Abbadini M, Gaboli M, Marchisio PC. Fibronectin and vitronectin regulate the organization of their respective Arg-Gly-Asp adhesion receptors in cultured human endothelial cells. J Cell Biol 1988; 107: 1215–1223.

    Article  CAS  PubMed  Google Scholar 

  • Donaldson DJ, Mahan JT, Smith GN Jr. Newt epidermal cell migration in vitro and in vivo appears to involve Arg-Gly-Asp-Ser receptors. J Cell Sci 1987; 87: 525–534.

    CAS  PubMed  Google Scholar 

  • Fitzgerald LA, Poncz M, Steiner B, Rall SC Jr., Bennet JS, Phillips DR. Comparison of the cDNA-derived protein sequences of the human fibronectin and vitronectin receptor alpha subunits and platelet glycoprotein IIb. Biochemistry 1987; 26: 8158–8165.

    Article  CAS  PubMed  Google Scholar 

  • Gailit J, Ruoslahti E. Regulation of the fibronectin receptor affinity by divalent cations. J Biol Chem 1988; 263: 12927–12932.

    CAS  PubMed  Google Scholar 

  • Gehlsen KR, Argraves WS, Pierschbacher MD, Ruoslahti E. Inhibition of in vitro tumor cell invasion by Arg-Gly-Asp-containing synthetic peptides. J Cell Biol 1988; 106: 925–930.

    Article  CAS  PubMed  Google Scholar 

  • Hayman EG, Pierschbacher MD, Ruoslahti E. Detachment of cells from culture substrate by soluble fibronectin peptides. J Cell Biol 1987; 100: 1948–1954.

    Article  Google Scholar 

  • Hiscott PS, Grierson I, McLeod D. Natural history of fibrocellular epiretinal membranes: a quantitative, autoradiographic, and immunohistochemical study. Br J Ophthalmol 1985; 69: 810–823.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hynes RO. Integrins: a family of cell surface receptors. Cell 1987; 48: 549–554.

    Article  CAS  PubMed  Google Scholar 

  • Juliano RL. Membrane receptors for extracellular matrix macromolecules: relationship to cell adhesion and tumor metastasis. Biochim Biophys Acta 1987; 907: 261–278.

    CAS  PubMed  Google Scholar 

  • Nishida T, Nakagawa S, Watanabe K, Yamada KM, Otori T, Berman MB. A peptide from fibronectin cell-binding domain inhibits attachment of epithelial cells. Invest Ophthalmol Vis Sci 1988; 29: 1820–1825.

    CAS  PubMed  Google Scholar 

  • Pierschbacher MD, Ruoslahti E. Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule. Nature 1984; 309: 30–33.

    Article  CAS  PubMed  Google Scholar 

  • Pierschbacher MD, Ruoslahti E. Influence of stereochemistry of the sequence Arg-Gly-Asp-Xaa on binding specifity in cell adhesion. J Biol Chem 1987; 262: 17703–17711.

    Google Scholar 

  • Poncz M, Eisman R, Heidenreich R, Silver SM, Vilaire G, Surrey S, Schwartz E, Benett JS. Structure of the platelet membrane glycoprotein IIb. Homology to the alpha subunits of the vitronectin and fibronectin membrane receptors. J Biol Chem 1987; 262: 8476–8482.

    CAS  PubMed  Google Scholar 

  • Pytela R, Pierschbacher MD, Ginsberg MH, Plow EF, Ruoslahti E. Platelet membrane glycoprotein IIb/IIIa: member of a family of Arg-Gly-Asp-specific adhesion receptors. Science 1986; 28: 1559–1562.

    Article  Google Scholar 

  • Ruoslahti E, Pierschbacher MD. Arg-gly-asp: a versatile cell recognition signal. Cell 1986; 44: 517–518.

    Article  CAS  PubMed  Google Scholar 

  • Ruoslahti E, Pierschbacher MD. New perspectives in cell adhesion: RGD and integrins. Science 1987; 238: 491–497.

    Article  CAS  PubMed  Google Scholar 

  • Scheiffarth OF, Kampik A, Günther H, von der Mark K. Proteins of the extracellular matrix in vitreoretinal membranes. Graefe's Arch Clin Exp Ophthalmol 1988; 226: 357–361.

    Article  CAS  Google Scholar 

  • Singer II, Scott S, Kawka DW, Kazazis DM, Gailit J, Ruoslahti E. Cell surface distribution of fibronectin and vitronectin receptors depends on substrate composition and extracellular matrix accumulation. J Cell Biol 1988; 106: 2171–2182.

    Article  CAS  PubMed  Google Scholar 

  • Sosnoski DM, Emanuel BS, Hawkins AL, van Tuinen P, Ledbetter DH, Nussbaum RL, Kaos FT, Schwartz E, Philipps D, Bennett JS, Fitzgerald LA, Poncz M. Chromosomal localization of the genes for the vitronectin and fibronectin receptors alpha subunits and for platelet glycoproteins IIb and IIIa. J Clin Invest 1988; 81: 1993–1998.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Thiagarajan P, Kelly KL. Exposure of binding sites for vitronectin on platelets following stimulation. J Biol Chem 1988; 263: 3035–3038.

    CAS  PubMed  Google Scholar 

  • Weller M, Heimann K, Wiedemann P. Immunochemical studies of epiretinal membranes using APAAP complexes. Evidence for macrophage involvement in traumatic proliferative vitreoretinopathy. Int Ophthalmol 1988a; 11: 181–186.

    Article  CAS  PubMed  Google Scholar 

  • Weller M, Heimann K, Wiedemann P. Immunochemical analysis of periretinal membranes. Review and outlook. In: Straub W (ed.) Developments in Ophthalmology Vol. 16, 1989a, pp. 54–74.

  • Weller M, Wiedemann P, Bresgen M, Heimann K. Vitronectin and proliferative intraocular disorders. I. A colocalisation of the serum spreading factor, vitronectin, and fibronectin in traction membranes from patients with proliferative vitreoretinopathy. Int Ophthalmol 1990; 15: 93–101.

    Article  Google Scholar 

  • Weller M, Wiedemann P, Heimann K, Zilles K. The significance of fibronectin in vitreoretinal pathology: a critical evaluation. Graefe's Arch Clin Exp Ophthalmol 1988b; 226: 294–298.

    Article  CAS  Google Scholar 

  • Weller M, Wiedemann P, Moter H, Heimann K. Transferrin and transferrin receptor expression in intraocular proliferative disease. APAAP-immunolabeling of retinal membranes and ELISA for vitreal transferrin. Graefe's Arch Clin Exp Ophthalmol 1989b; 227: 281–286.

    Article  CAS  Google Scholar 

  • Wiedemann P, Weller M. The pathophysiology of proliferative vitreoretinopathy. Acta Ophthalmol 1988; 66: Suppl. 189.

    Google Scholar 

  • Yamada KM, Kennedy DW. Peptide inhibitors of fibronectin, laminin, and other adhesion molecules: unique and shared features. J Cell Physiol 1987; 130: 21–28.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Vitreoretinal Surgery, University Eye Clinic Cologne, FRG

    Michael Weller, Peter Wiedemann, Martin Bresgen & Klaus Heimann

Authors
  1. Michael Weller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter Wiedemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Martin Bresgen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Klaus Heimann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Weller, M., Wiedemann, P., Bresgen, M. et al. Vitronectin and proliferative intraocular disorders. II. Expression of cell surface receptors for fibronectin and vitronectin in periretinal membranes. Int Ophthalmol 15, 103–108 (1991). https://doi.org/10.1007/BF00224462

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00224462

Key words

Search

Navigation