Abstract
Proliferative vitreoretinopathy (PVR) is characterized by cellular proliferation and the formation of intravitreal and periretinal membranes predisposing to traction retinal detachment. Recently, fibronectin, a high-molecular glycoprotein, has been attributed an important role in the pathogenesis of PVR. In order to evaluate the significance of fibronectin as a diagnostic or prognostic marker for PVR, we determined levels of immunoreactive fibronectin in 59 human vitreous specimens by a noncompetitive ELISA technique. Before this study, fibronectin levels in the vitreous had not been investigated taking both protein and iron levels into consideration. The vitreous was obtained during vitrectomies performed for a broad spectrum of vitreoretinal disorders. Our measurements suggest that the amount of intravitreal fibronectin may be correlated with the severity of a manifested disorder rather than being diagnostic for PVR. As long as plasma-derived fibronectin cannot be differentiated from other tissue fibronectins, however, the concentration of this glycoprotein is not a reliable indicator of intraocular proliferative activity. The values for all three parameters — fibronectin, protein, and iron — were significantly higher in revitrectomy samples compared to patients undergoing a first vitrectomy. A somewhat surprising result was a high level of fibronectin in the vitreous of two patients with macular pucker.
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References
Campochiaro PA, Jerdan JA, Glaser BM, Cardin A, Michels RG (1985) Vitreous aspirates from patients with proliferative vitreoretinopathy stimulate retinal pigment epithelial cell migration. Arch Ophthalmol 103:1403–1405
Cooper WC, Halbert SP, Manski WJ (1963) Immunochemical analysis of vitreous and subretinal fluid. Invest Ophthalmol 2:369–377
Criswick VG, Schepens Cl (1969) Familial exudative vitreoretinopathy. Am J Ophthalmol 68:578–584
Gomez-Lechon MJ, Castell JV (1985) Enzyme-linked immunosorbent assay to quantify fibronectin. Anal Biochem 145:1–8
Gressner AM, Wallraff P (1980) Laser nephelometric procedure for the determinaton of the fibronectin concentration in plasma. J Clin Chem Clin Biochem 18:797–805
Heimann K, Paulmann H, Tavakolian U (1983) The intraocular foreign body. Principles and problems in the management of complicated cases by pars plana vitrectomy. Int Ophthalmol 6: 235–242
Kampik A, Kenyon KR, Michels RG, Green WR, Cruz ZC de la (1981) Epiretinal and vitreous membranes. Comparative study of 56 cases. Arch Ophthalmol 99: 1445–1454
Laemmii UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Towbin H, Gordon J (1984) Immunoblotting and Dot immunobinding — current status and outlook. J Immunol Methods 72:313–340
Vuento M, Salonen E, Salminen K, Pasanen M, Stenman U (1980) Immunochemical characterization of human plasma fibronectin. Biochem J 191:719–727
Wiedemann P, Heimann K (1986) Proliferative Vitreoretinopathie: Pathogenese und Möglichkeiten der Beeinflussung mit Zytostatika. Klin Monatsbl Augenheilkd 188:559–564
Yamada KM, Akiyama SK, Hasegawa T, Hasegawa E, Humphries MJ, Kennedy DW, Nagata K, Urushihara H, Olden K, Chen W (1985) Recent advances in research on fibronectin and other cell attachment proteins. J Cell Biochem 28: 79–97
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This study was supported by the Retinovit Foundation and the Forschungsförderung Nordrhein-Westfalen
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Weller, M., Wiedemann, P., Heimann, K. et al. The significance of fibronectin in vitreoretinal pathology. Graefe's Arch Clin Exp Ophthalmol 226, 294–298 (1988). https://doi.org/10.1007/BF02181200
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DOI: https://doi.org/10.1007/BF02181200