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Functional polymers from (vinyl)polystyrene. Enzyme immobilization through a cysteinyl-S-ethyl spacer

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Biotechnology Techniques

Abstract

By radical-catalyzed addition to residual vinyl groups, cysteine was S-linked to porous beads of divinylbenzene/ethylstyrene copolymer. Subsequent treatment with acetic anhydride gave polystyrene-supported azlactone groups able to covalently bind enzymes out of aqueous solution under mild conditions. Pseudomonas cepacia lipase thus immobilized remained active for the hydrolysis of esters, even after storage and/or several cycles of use and recovery.

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References

  • Bartholin, M.; Boissier, G.; Dubois, J. (1981). J. Makromol Chem. 182, 2075–2085.

    Google Scholar 

  • Brzozowski, A.M.; Derewenda, U.; Derewenda, Z.S.; Dodson, G.G.; Lawson, D.M.; Turkenburg, J.P.; Bjorkling, F.; Huge-Jensen, B.; Patkar, S.A.; Thim, L. (1991). Nature 351, 491–494.

    Google Scholar 

  • Chibata, I.; Tosa, T.; Sato, T. (1983). Adv. Biotech. Proc. 1, 203–222.

    Google Scholar 

  • Darling, G.D.; Fréchet, J.M.J. (1986). J. Org. Chem. 51, 2270–2276.

    Google Scholar 

  • Deratani, A.; Darling, G.D.; Horak, D.; Fréchet, J.M.J. (1987). Macromolecules 20, 767.

    Google Scholar 

  • Gao, J.P.; Morin, F.G.; Darling, G.D. (1993). Macromolecules 26, 1196–1198.

    Google Scholar 

  • Goldstein, L.; Manecke, G. (1976). Appl. Biochem. Bioeng. 1, 23–126.

    Google Scholar 

  • Heilmann, S.M.; Rasmussen, J.K.; Krepski, L.R.; Milbraith, D.S. (1988). U.S. Patent 4,737,560; Chem. Abstr. 109, P93880u.

  • Hsu, S.H.; Wu, S.S.; Wang, Y.F.; Wong C.H. (1990). Tetrahedron Lett. 31, 6403–6406.

    Google Scholar 

  • Jones, J.B. (1986). Tetrahedron Lett. 42, 3351–3403.

    Google Scholar 

  • Lowry, D.H.; Rosenbrough, N.J.; Farr, A.L.; Randall, R.J.J. (1951). Biochemistry 193, 265.

    Google Scholar 

  • Mosbach, K. (ed.) (1980). Meth. Enzymol. 44, passim.

  • Peterson, G.L. (1977). Anal Biochem. 83, 346.

    Google Scholar 

  • Sherrington, D.C.; Hodge, P. (1988). Syntheses and separations using functional polymers, New York: Wiley.

    Google Scholar 

  • Stranix, B.R.; Gao, J.P.; Barghi, R.; Darling G.D. (1994). In preparation for J. Org. Chem.

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Authors and Affiliations

  1. Department of Chemistry, McGill University, 801 Sherbrooke Street West, H3A 2K6, Montreal, Québec, Canada

    Brent R. Stranix & Graham D. Darling

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  1. Brent R. Stranix
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  2. Graham D. Darling
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Stranix, B.R., Darling, G.D. Functional polymers from (vinyl)polystyrene. Enzyme immobilization through a cysteinyl-S-ethyl spacer. Biotechnol Tech 9, 75–80 (1995). https://doi.org/10.1007/BF00224401

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