Summary
Three sites of somatostatin-synthesizing perikarya, or a related antigen, were determined by immunofluorescence in the hypothalamus of the tadpole, Alytes obstetricans (Amphibia, Anura). Two sites of neurosecretory perikarya were localized in the preoptic nuclei of the anterior hypothalamus; the axons extended either to the anterior diencephalon or to the median eminence and the pituitary. The third site was found in the posterior hypothalamus. These neurosecretory cells showed a strong immunofluorescent reaction; their axons all terminated at the level of the median eminence. Somatostatin cells were only found in intact or hypophysectomized tadpoles given somatotropin (STH). The strong reaction observed in hypophysectomized tadpoles was possibly due to the loss of the terminal portion of the neurosecretory pathway (median eminence and pituitary) by which the agent is transported to the site of discharge.
Similar content being viewed by others
References
Brazeau, P., Vale, W., Burgus, R., Ling, N., Butcher, M., Rivier, J., Guillemin, R.: Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary secretion of immunoreactive pituitary growth hormone. Science 179, 77–79 (1973)
Calas, A., Dubois, M.P., Assenmacher, I.: Exploration immunocytologique de Péminence médiane du canard avec des I.S. contre la LH-RH et la somatostatine, couplées avec une sérum albumine humaine. J. Physiol. (Paris) 13 B (1975)
Doerr-Schott, J., Dubois, M.P.: Répartition comparée des structures réagissant en immunofluorescence avec des anticorps anti-somatostatine, anti-neurophysine et anti-LH-RF chez deux Amphibiens: Le crapaud et le Xénope. J. Physiol. (Paris) 72, 5 B (1976)
Dubois, M.P.: Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas. Proc. nat. Acad. Sci. (Wash.) 72, 4, 1340–1343 (1975)
Dubois, M.P., Barry, J., Léonardelli, J.: Mise en évidence par immunofluorescence et répartition de la somatostatine (SRIF) dans l'éminence médiane des Vertébrés (Mammifères, Oiseaux, Amphibiens, Poissons). C.R. Acad. Sci. (Paris) 279, 1899–1902 (1974)
Dubois, M.P., Kolodziejczyk, E.: Centres hypothalamiques du rat sécrétant la somatostatine: répartition des péricaryons en 2 systèmes magno-et parvocellulaires. (étude immunocytologique). C.R. Acad. Sci. (Paris) 281, 1737–1740 (1975)
Rémy, Ch.: Effets de l'ablation du complexe pituitaire chez le têtard du Crapaud accoucheur Alytes obstetricans. Laur. C.R. Acad. Sci. (Paris) 258, 2912–2915 (1964)
Rémy, Ch.: Contribution à l'étude expérimentale des mécanismes neuro-endocriniens intervenant dans la morphogenèse et la croissance des têtards d'Alytes obstetricans. Laur. Thèse Sci. nat. AO 3603 (1969)
Vale, W., Brazeau, P., Grant, G., Nussey, A., Burgus, R., Rivier, J., Ling, N., Guillemin, R.: Premières observations sur le mode d'action de la somatostatine, un facteur hypothalamique qui inhibe la sécrétion de l'hormone de croissance. C.R. Acad. Sci. (Paris) 275, 2913–2915 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rémy, C., Dubois, M.P. Immunofluorescence of somatostatin-producing sites in the hypothalamus of the tadpole, Alytes obstetricans Laur. . Cell Tissue Res. 187, 315–321 (1978). https://doi.org/10.1007/BF00224373
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224373