Summary
Modifications of cell shape induced in cultured newborn rat astroblasts by serum deprivation or dibutyryladenosine 3′–5′ monophosphate (dBcAMP) are described. Serum suppression modifies the adhesivity of the cells to the substrate, but this modification is not consistent with a true differentiation. The main ultrastructural feature of dBcAMP-treated astroblasts is the presence of an extensive system of 90 Å microfilaments, while control cells are relatively devoid of these structures.
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References
Edström, A., Kanje, M., Walum, E.: Effects of dibutyryl cyclic AMP and prostaglandin E1 on cultured human glioma cells. Exp. Cell Res. 85, 217–223 (1974)
Grobstein, C.: Differentiation of vertebrate cells. The cell (Brachet, J. and Mirsky, A. eds.), vol. 1, p. 437–496. New York: Academic Press 1958
Klier, G., Schubert, D., Heinemann, S.: Ultrastructural changes accompanying the induced differensstiation of clonal rat nerve and glia. Neurobiology 5, 1–37 (1975)
Lim, R., Mitsunobu, K., Li, W.K.P.: Maturation-stimulating effect of brain extract and dibutyryl cyclic AMP on dissociated embryonic brain cells in culture. Exp. Cell Res. 79, 243–246 (1973)
McIntyre, E.H., Wintersgill, Č.J., Perkins, J.P., Vatter, A.E.: The responses in culture of human tumour astrocytes and neuroblasts to N6,O2-dibutyryl adenosine 3′,5′-monophosphoric acid. J. Cell Sci. 11, 639–667 (1972)
Moonen, G., Cam, Y., Sensenbrenner, M., Mandel, P.: Variability of the effects of serum-free medium, dibutyryl-cyclic AMP or theophylline on the morphology of cultured newborn rat astroblasts. Cell Tiss. Res. 163, 365–372 (1975)
Schubert, D.: Induced differentiation of clonal rat nerve and glial cells. Neurobiology 4, 376–387 (1975)
Shapiro, D.L.: Morphological and biochemical alterations in foetal rat brain cells cultured in the presence of monobutyryl cyclic AMP. Nature (Lond.) 241, 203–204 (1973)
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Dr. G. Moonen is Aspirant du Fonds National de la Recherche Scientifique.
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Moonen, G., Heinen, E. & Goessens, G. Comparative ultrastructural study of the effects of serum-free medium and dibutyryl-cyclic AMP on newborn rat astroblasts. Cell Tissue Res. 167, 221–227 (1976). https://doi.org/10.1007/BF00224329
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DOI: https://doi.org/10.1007/BF00224329