Summary
A series of hexaploid wheat lines containing zero, two or four doses of rye chromosome arm 1RS was used to investigate the response to changes in dosage by the rye genes when in a wheat background. The quantity of protein produced by the secalin protein genes contained on 1RS was directly related to the number of copies of 1RS present in the line. No response could be detected by representative wheat proteins suggesting that the increase in secalin protein observed was due to an increase in mRNA produced when four copies of the secalin gene was present. These results suggest that increasing the dosage of alien genes introgressed into wheat may be a useful tool for enhancing their expression.
Mention of a trade name or proprietary product does not constitute a guarantee, warranty or recommendation of the product by the U.S. Department of Agriculture or the University of Missouri and does not imply its approval to the exclusion of other products that may be suitable.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aragoncillo C, Rodriguez-Loperena MA, Salcedo G, Carbonero P, Garcia-Olmedo F (1978) Influence of homeologous chromosomes on gene dosage effects in allohexaploid wheat (Triticum aestivum L.). Proc Natl Acad Sci USA 75:1446–1450
Bartels D, Thompson RD (1986) Synthesis of mRNAs coding for abundant endosperm proteins during wheat grain development. Plant Sci 46:117–125
Bittel DC, Fominaya A, Jouve N, Gustafson JP (1991) Dosage response of rye genes in a wheat background 1. Selection of lines with multiple copies of 1RS, J Plant Breed
Bradford M (1976) A rapid and sensitive method for the determination of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Galili G, Feldman M (1984) Intergenomic suppression of endosperm protein genes in common wheat. Can J Genet Cytol 26:651–656
Galili G, Levy AA, Feldman M (1986) Gene-dosage compensation of endosperm proteins in hexaploid wheat Triticum aestivum. Proc Natl Acad Sci USA 83:6524–6528
Gustafson JP (1988) Evaluation of a 1R(1D) substitution in wheat. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Cambridge, UK, pp 193–196
Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR (1988) The structure and expression of genes encoding the high molecular weight (HMW) subunits of wheat glutenin. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Cambridge, UK, pp 745–750
Lukaszewski AJ, Gustafson JP (1983) Translocations and modifications in triticale x wheat hybrids. Theor Apl Genet 64:239–248
Ng PKW, Scanion MG, Bushuk W (1988) A catalog of biochemical fingerprints of registered Canadian wheat cultivars by electrophoresis and high-performance liquid chromatography. University of Manitoba, Winnipeg, Canada
Rajaram S, Mann CE, Ortiz-Ferrara G, Mujeeb-Kazi A (1983) Adaptation, stability and high yield potential of certain 1B/1R CIMMYT wheats. In: Sakamoto S (ed) Proc 6th Int Wheat Genet Symp. Kyoto, Japan, pp 613–621
Rajaram S, Villareal R, Mujeeb-Kazi A (1990) The global impact of 1B/1R wheats. Agronomy Abstr 105. Annual Meeting ASA, CSSA, SSA
Rogers WJ, Rickatson JM, Sayers EJ, Law CN (1990) Dosage effects of chromosomes of homoeologous groups 1 and 6 upon bread making quality in hexaploid wheat. Theor Appl Genet 80:281–287
Sebesta EE, Wood Jr EA (1978) Transfer of greenbug resistance from rye to wheat with X-rays. Agronomy Abstr 61. Annual Meeting ASA, CSSA, SSA
Zeller FJ, Hsam SLK (1983) Broadening the genetic variability of cultivated wheat by utilizing rye chromatin. In: Sakamoto S (ed) Proc 6th Int Wheat Genet Symp. Kyoto, Japan, pp 161–173
Author information
Authors and Affiliations
Additional information
Communicated by J. W. Snape
Contribution from the Missouri Agricultural Experiment Station. Journal Series No. 11,413
Rights and permissions
About this article
Cite this article
Bittel, D.C., Gustafson, J.P. Dosage response of rye genes in a wheat background. Theoret. Appl. Genetics 84, 1–5 (1992). https://doi.org/10.1007/BF00223973
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223973