Abstract
Among pale-green tomato plants heterozygous for the xanthophyllic2 (xa-2) mutation that were transformed with a T-DNA harbouring the NPTII and GUS gene, a plant with a high frequency of green/white twin spots was found. The genetic analysis of this plant indicated that the occurrence of these twin spots was caused by a genetic defect located at the distal end of chromosome 10S, where xa-2 also is located. The genetic analysis of green plants regenerated from leaf expiants of this twin-spot plant revealed that the green sectors derive from non-disjunction of the xa-2 + allele. In an analysis of mitotic chromosome behaviour bridges were observed in approximately 5% of the anaphases, providing arguments that a breakage-fusion-bridge cycle caused by a tissue culture-induced genomic instability is the most likely cause of this aberrant behaviour of chromosome 10.
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References
Carlson PS (1974) Mitotic crossing-over in a higher plant. Genet Res 24:109–112
Evans DA, Paddock EF (1976) Comparisons of somatic crossing over frequency in Nicotiana tabacum and three other crop species. Can J Genet Cytol 18:57–65
Evans DA, Paddock EF (1980) Characterization of X-ray induced increase of mitotic cross-overs in Glycine max. Theor Appl Genet 56:245–251
Evans DA, Bravo JE, Kut SA, Flick CE (1983) Genetic behaviour of somatic hybrids in the genus Nicotiana: N. otophora + N. tabacum and N. sylvestris + N. tabacum. Abstr 6th Int Protoplast Symp. Birkhauser Verlag, Basel, pp 98–99
Gröber K (1963) Somatische Spaltung in dem heterozygoten Genotyp der dominanten Chlorophyllmutation Xanthophyllic 3 von Lycopersicon esculentum Mill. I. Erste Genetische und Cytologische Beobachtungen in der X1-Generation. Kulturpflanze 11:583–602
Gröber K (1967) Somatische Spaltung in dem heterozygoten Genotyp der dominanten Chlorophyllmutation Xanthophyllic 3 von Lycopersicon esculentum Mill. II. Cytogenetische Analyse der verschiedene Schreckungstypen in der X2 und X3 Generation. Kulturpflanze 15:351–365
Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907
Koornneef M, Stam P (1992) Genetic analysis. In: Koncz C, Chua NH, Schell J (eds) Methods in Arabidopsis research. World Scientific, Singapore, pp 83–99
Koornneef M, Hanhart CJ, Martinelli L (1987a) A genetic analysis of cell-culture traits in tomato. Theor Appl Genet 74:633–641
Koornneef M, Jongsma M, Weide R, Zabel P, Hille J (1987b) Transformation of tomato. In: Nevins DJ, Jones RA (eds) Tomato biotechnology. Alan R. Liss, New York, pp 169–178
Koornneef M, van Diepen JAM, Hanhart CJ, Kieboom-de Waart AC, Martinelli L, Schoenmakers HCH, Wijbrandi J (1989) Chromosomal instability in cell and tissue cultures of tomato haploids and diploids. Euphytica 43:179–186
Koornneef M, Bade J, Hanhart C, Horsman K, Schel J, Soppe W, Verkerk R, Zabel P (1993) Characterization and mapping of a gene controlling shoot regeneration in tomato. Plant J 3:131–141
Lee M, Phillips RL (1988) The chromosomal basis of somaclonal variation. Annu Rev Plant Physiol Mol Biol 39:413–437
Lörz H, Scowcroft WR (1983) Variability among plants and their progeny regenerated from protoplasts of Su/su heterozygotes of Nicotiana tabacum. Theor Appl Genet 66:67–75
Parag Y (1977) Genetic analysis of mutations for low (rec) and very high (pop) mitotic-recombination frequency in Aspergillus nidulans. Mol Gen Genet 155:319–327
Persson AR (1960) Physiological and genetical investigations on chlorophyll mutants. Rep Tomato Genet Coop 10:27–28
Peterson PW, Yoder JI (1993) Ac-induced instability at the xanthophyllic locus of tomato. Genetics 134:931–942
Ramanna MS, Hille J, Zabel P (1985) Chromosome breakage-fusion bridge-cycle and phenotypic instability in isochromosome lines of tomato. Theor Appl Genet 71:145–152
Schoenmakers HCH, Koornneef M, Alefs SJHM, Gerrits WFM, van der Kop, Chérel I, Caboche M (1991) Isolation and characterization of nitrate reductase-deficient mutants in tomato (Lycopersicon esculentum Mill.). Mol Gen Genet 227:458–464
Seeni S, Gnanam A (1981) In vitro regeneration of chlorophyll chimeras in tomato (Lycopersicon esculentum). Can J Bot 59:1941–1943
Tanksley SD (1993) Linkage map of tomato (Lycopersicon esculentum) (2N = 24) In: O'Brien SJ (ed) Genetic maps: locus maps of complex genomes, 6th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y., pp 6.39–6.60
Vig BK (1969) Relationship between mitotic events and leaf spotting in Glycine max. Can J Genet Cytol 11:147–152
Vig BK (1973) Somatic crossing-over in Glycine max (L.) Merrill: mutagenicity of sodium azide and lack of synergistic effect with caffeine and mitomycin C. Genetics 75:265–277
Weide R, Koornneef M, Zabel P (1989) A Simple, non-destructive spraying assay for the detection of an active kanamycin resistance gene in transgenic tomato plants. Theor Appl Genet 78:169–172
Wolters AMA, Vergunst AC, van der Werff F, Koornneef M (1993) Analysis of nuclear and organellar DNA of somatic hybrid calli and plants between Lycopersicon spp. and Nicotiana spp. Mol Gen Genet 241:707–718
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Zhu, D., Schoenmakers, H.C.H., Wolters, A.M.A. et al. A genetic analysis of a tomato (Lycopersicon esculentum) genotype with a high frequency of twin spots. Theoret. Appl. Genetics 91, 1162–1166 (1995). https://doi.org/10.1007/BF00223935
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DOI: https://doi.org/10.1007/BF00223935