Summary
Three lines derived from wheat (6x) x Agropyron elongatum (10x) that are resistant to wheat streak mosaic virus (WSMV) were analyzed by chromosome pairing, banding, and in situ hybridization. Line CI15321 was identified as a disomic substitution line where wheat chromosome 1D is replaced by Ag. elongatum chromosome 1Ae-1. Line 87-94-1 is a wheat-Ag. elongatum ditelosomic addition 1Ae-1L. Line CI15322 contains an Ag. elongatum chromosome, 1Ae-2, that substitutes for chromosome 1D. The short arm of 1Ae-2 paired with the short arm of 1Ae-1 at metaphase I (MI) in 82% of the pollen mother cells (PMCs). However, the long arms of these two chromosomes did not pair with each other. In CI15322, the long arm of chromosome 4D has an Agropyron chromosome segment which was derived from the distal part of 1Ae-1L. This translocation chromosome is designated as T4DS·4DL-1L. T4DS·4DL-1Ae-1L has a 0.73 μm distal part of the long arm of 4D replaced by a 1.31 μm distal segment from 1Ae-1L. The major WSMV resistance gene(s) in these lines is located on the distal part of 1Ae-1L.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Friebe B, Mukai Y, Dhaliwal HS, Martin TJ, Gill BS (1991) Identification of alien chromatin specifying resistance to wheat streak mosaic and greenbug in wheat germ plasm by C-banding and in situ hybridization. Theor Appl Genet 81:381–389
Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885
Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum L.) Genome 34:830–839
Larson RI, Atkinson TG (1970) Identify of the wheat chromosome replaced by Agropyron chromosomes in a triple alien chromosome substitution line immune to wheat streak mosaic. Can J Genet Cytol 12:145–150
Larson RI, Atkinson TG (1973) Wheat-Agropyron chromosome substitution lines as sources of resistance to wheat streak mosaic virus and its vector, Aceria tulipae. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp. University of Missouri, Columbia, Mo. pp 173–184
Lay CL, Wells DG, Gardner WAS (1971) Immunity from wheat streak mosaic virus in irradiated Agrotriticum progenies. Crop Sci 11:431–432
Le HT, Armstrong KC, Miki B (1989) Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. Plant Mol Biol Rep 7:150–158
Liang GH, Wang RC, Niblett CL, Heyne EG (1979) Registration of B-6–37–1 wheat germ plasm. Crop Sci 19:421
Martin TJ, Harvey TL, Livers RW (1976) Resistance to wheat streak mosaic virus and its vector, Aceria tulipae. Phytopathology 66:346–349
Muramatsu M (1990) Cytogenetics of decaploid Agropyron elongatum (Elytrigia elongata) (2n = 70). I. Frequency of deca valent formation. Genome 33:811–817
McKinney HH, Sando WJ (1951) Susceptibility and resistance to the wheat streak mosaic virus in the genera Triticum, Agropyron, Secale, and certain hybrids. Plant Dis Rep 35:476–478
Mukai Y, Gill BS (1991) Detection of barley chromatin added to wheat by genomic in situ hybridization. Genome 34:448–452
Pfannenstiel MA, Niblett CL (1978) The nature of the resistance of Agrotriticum to wheat streak mosaic virus. Phytopathology 68:1204–1209
Rayburn AL, Gill BS (1985) Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes. J Hered 76:78–81
Sebesta EE, Bellingham RC (1963) Wheat viruses and their genetic control. In: MacKey J (ed) Proc 2nd Int Wheat Genet Symp. Lund, Sweden, Hereditas (Suppl) 2:184–201
Sebesta EE, Young HC, Wood EA (1972) Wheat streak mosaic virus resistance. Annu Wheat Newsl 18:136
Sharma HC, Gill BS, Uyemoto JK (1984) High level of resistance in Agropyron species to barley yellow dwarf and wheat streak mosaic virus. Phytopathol Z 110:143–147
Sill Jr WH, Bellingham RC, Fellows H (1964) Reaction of wheat, wheat crosses, barley, rye and oats to wheat streak mosaic virus. Kan State Univ Agric Exp Sta Tech Bull 132
Sim IV T, Willis WG, Eversmeyer MG (1988) Kansas plant disease survey. Plant Dis 72:832–836
Somsen HW, Sill Jr WH (1970) The wheat curl mite, Aceria ulipae Keifer, in relation to epidemiology and control of wheat streak mosaic. Kans State Univ Agric Exp Sta Res Publ 162
Stoddard SL, Gill BS, Lommel SA (1987a) Genetic expression of wheat streak mosaic virus resistance in two wheatwheatgrass hybrids. Crop Sci 27:514–519
Stoddard SL, Lommel SA, Gill BS (1987b) Evaluation of wheat germ plasm for resistance to wheat streak mosaic virus by symptomatology, ELISA, and slot-blot hybridization. Plant Dis 71:714–719
Wang RC, Liang GH (1977) Cytogenetic location of genes for resistance to wheat streak mosaic in an Agropyron substitution line. J Hered 68:375–378
Wells DG, Wong R Sze-Chung, Lay CL, Gardner WS, Buchenau GW (1973) Registration of C.I. 15092 and C.I. 15093 wheat germ plasm. Crop Sci 13:776
Wells DG, Kota RS, Sandhu HS, Garnder WS, Finney KF (1982) Registration of one disomic substitution line and five translocation lines of winter wheat germ plasm resistant to wheat streak mosaic virus. Crop Sci 22:1277–1278
Whelan EDP, Hart GE (1988) A spontaneous translocation that transfers wheat curl mite resistance from decaploid Agropyron elongatum to common wheat. Genome 30:289–292
Whelan EDP, Atkinson TG, Larson RI (1983) Registration of LRS-IF 193 wheat germplasm. Crop Sci 23:194
Author information
Authors and Affiliations
Additional information
Communicated by J. W. Snape
Contribution No. 92-599-J from the Kansas Agricutural Experiment Station, Kansas State University, Manhattan, Kansas, USA
Rights and permissions
About this article
Cite this article
Jiang, J., Friebe, B., Dhaliwal, H.S. et al. Molecular cytogenetic analysis of Agropyron elongatum chromatin in wheat germplasm specifying resistance to wheat streak mosaic virus. Theoret. Appl. Genetics 86, 41–48 (1993). https://doi.org/10.1007/BF00223806
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223806