Summary
The obligate parasitic fungus, Erysiphe graminis f. sp. hordei, was found to harbour plasmid-like extrachromosomal DNA. A 1.35-kb fragment of this 9kb plasmid was cloned into the pUC12 vector. No homology was detected to nuclear or mitochondrial DNA. As only about half of the 27 isolates examined contained plasmid-like DNA, this appears to be inessential for fungal survival. The plasmid is frequent in European isolates and is found in both newly collected isolates and in isolates kept under laboratory conditions for many years. No correlation between presence of plasmid and specific avirulence/virulence genes was found. The plasmid appear to be located in the mitochondria.
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References
Birmboim HC, Doly I (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Chan VTW, Fleming KA, McGee JO'D (1985) Detection of subpicogram quantities of specific DNA sequences on blot hybridization with biotinylated probes. Nucleic Acids Res 13, 22:8083–8091
Dixon LK, Leaver CJ (1982) Mitochondrial gene expression and cytoplasmic male sterility in sorghum. Plant Mol Biol 1:89–102
Düvell A, Hessberg-Stutzke H, Oeser B, Rogmann-Backwinkel P, Tudzynski P (1988) Structural and functional analysis of mitochondrial plasmids in Claviceps purpurea. Mol Gen Genet 214:128–134
Eckhardt T (1978) A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid 1:584–588
Flor HH (1955) Host-parasite interactions in flax rust — Its genetics and other implications. Phytopathology 45:680–685
Francon F (1981) Isolation and characterization of a linear DNA molecule in the fungus Ascobolus immersus. Mol Gen Genet 184:440–444
Garber RC, Turgeon BG, Yoder OC (1984) A mitochondrial plasmid from the plant pathogenic fungus Cochliobolus heterostrophus. Mol Gen Genet 196:301–310
Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885
Giasson L, Lalonde M (1987) Analysis of a linear plasmid isolated from the pathogenic fungus Ceratocystis fimbriata Ell & Halst. Curr Genet 11:331–334
Hashiba T (1987) Plasmids responsible for pathogenicity and morphology in Rhizoctonia solani. In: Nishimura S, Vance CP, Doke N (eds) Molecular determinants of plant diseases. Japan Scientific Society Press, Tokyo Springer, Berlin Heidelberg New York, pp 157–170
Hermansen JE (1980) A spontaneous mutation in Erysiphe graminis f. sp. hordei for virulence to host gene Ml-g. Phytopathol Z 98:171–177
Hiura U, Heta H (1955) Studies on the disease resistance in barley. III. Further studies on the physiologic races of Erysiphe graminis hordei in Japan. Ber Ohara Inst Landwirtsch Biol 10:135–156
Honeyman AL, Currier TC (1986) Isolation and characterization of linear DNA elements from the mitochondria of Gaeumannomyces graminis. Appl Env Microbiol 52:924–929
Jensen HP, Jørgensen JH (1981) Powdery mildew resistance in genes in Northwest European winter barley varieties. Dan J Plant Soil Sci 85:303–319
Kemble RJ (1987) A rapid, single-leaf, nucleic acid assay for determining the cytoplasmic organelle complement of rapeseed and related Brassica species. Theor Appl Genet 73:364–370
Kistler HC, Leong SA (1986) Linear plasmid-like DNA in the plant pathogenic fungus Fusarium oxysporum f. sp. conglutinans. J Bacteriol 167:587–593
Kølster P, Munk L, Stølen O, Løhde J (1986) Near-isogenic barley lines with genes for resistance to Erysiphe graminis f. sp. hordei. Crop Sci 26:903–907
Maniatis T, Fritsch EF, Sambrook I (1982) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory Press, Cold Spring Harbor/NY
Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78
Moseman JG (1959) Host-pathogen interaction of the genes for resistance in Hordeum vulgare and for pathogenicity in Erysiphe graminis f. sp. hordei. Phytopathology 49:469–472
Moseman JG (1968) Reactions of barley to Erysiphe graminis f. sp. hordei from North America, England, Ireland, and Japan. Plant Dis Rep 52:463–467
Samac DA, Leong SA (1988) Two linear plasmids in mitochondria of Fusarium solani f. sp. cucurbitae. Plasmid 19:57–67
Schwarzbach E (1979) Response to selection for virulence against the ml-o based mildew resistance in barley, not fitting the gene-for-gene hypothesis. Barley Genet Newsl 9:85–88
Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet 75:286–290
Stahl U, Lemke PA, Tudzynski P, Kück U, Esser K (1978) Evidence for plasmid-like DNA in a filamentous fungus, the ascomycete Podospora anserine. Mol Gen Genet 162:341–343
Stohl LL, Collins RA, Cole MD, Lambowitz AM (1982) Characterization of two new plasmid DNAs found in mitochondria of wild-type Neurospora intermedia strains. Nucleic Acids Res 10:1439–1458
Tudzynski P, Esser K (1985) Mitochondrial DNA for gene cloning in eukaryotes. In: Bennett JW, Lasure LL (eds) Gene manipulations in fungi. Academic Press, New York, pp 403–416
Tudzynski P, Düvell A, Esser K (1983) Extrachromosomal genetics of Claviceps purpurea. Curr Genet 7:145–150
Turpen T, Garger SI, Marks MD, Grill LK (1987) Molecular cloning and physical characterization of a Brassica linear mitochondrial plasmid. Mol Gen Genet 209:227–233
Wiberg A (1974) Sources of resistance to powdery mildew in barley. Hereditas 78:1–40
Zabala G, Walbot V (1988) An S1 episomal gene of maize mitochondria is expressed in male sterile and fertile plants of the S-type cytoplasm. Mol Gen Genet 211:386–392
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Giese, H., Christiansen, S.K. & Jensen, H.P. Extrachromosomal plasmid-like DNA in the obligate parasitic fungus Erysiphe graminis f. sp. hordei . Theoret. Appl. Genetics 79, 56–64 (1990). https://doi.org/10.1007/BF00223787
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DOI: https://doi.org/10.1007/BF00223787