Abstract
The megagametophytes of seeds of Pinus pinaster Ait. contain two types of oligomeric globulins of approximately 175 and 190 kDa that are comprised of 47-kDa and 27- and 22-kDa, monomers, respectively, joined by weak interactions. The 27- and 22-kDa components were purified and their N-terminal sequences determined. Both polypeptides were inherited as if they were coded by a single unit of recombination. The results obtained suggest that these two polypeptides originate from a single protein that undergoes proteolytic processing. The characteristics of this P. pinaster globulin indicate that it is a member of the 7S globulin family of seed storage proteins.
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References
Adams WT (1983) Application of isozymes in tree breeding. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier Science Publ, Amsterdam, pp 381–400
Allona I, Casado R, Aragoncillo C (1992) Seed storage proteins from Pinus pinaster Ait: homology of major components with 11S proteins from angiosperms. Plant Sci 87:9–18
Bailey NTJ (1961) Introduction to the mathematical theory of genetic linkage. Oxford University Press, London
Bidlingmeyer BA, Cohen SA, Tarvin TL (1984) Rapid analysis of amino acids using precolumn derivatization. J Chromatogr 336:93–104
Blakesley RW, Boezi JA (1977) A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G-250. Anal Biochem 82:580–582
Borroto K, Dure L (1987) The globulin seed-storage proteins of flowering plants are derived from two ancestral genes. Plant Mol Biol 8:113–131
Casey R, Domoney C, Ellis N (1986) Legume storage proteins and their genes. Oxford Surv Plant Mol Cell Biol 3:1–95
Chlan CA, Pyle JB, Legocki AB, Dure L (1986) Developmental biochemistry of cottonseed embryogenesis and germination. XVIII. cDNA and amino acid sequences of members of the storage protein families. Plant Mol Biol 7:475–489
Delaage M (1968) Sur la recherche du poid moleculaire le plus coherent avec l'analyse des acides aminés d'une proteine. Biochim Biophys Acta 168:573–575
Derbyshire E, Wright DJ, Boulter D (1976) Legumin and vicilin storage proteins of legume seeds. Phytochemistry 15:3–24
Domoney C, Casey R (1985) Measurement of gene number for seed storage proteins in Pisum. Nucleic Acids Res 13:687–699
Drysdale JW, Rigghetti P, Bunn HF (1971) The separation of human and animal haemoglobins by isoelectric focussing in polyacrylamide gel. Biochim Biophys Acta 229:42–50
Flinn BS, Roberts DR, Webb DT, Sutton BCS (1991) Storage protein changes during zygotic embryogenesis in interior spruce. Tree Physiol 8:71–81
Garcia-Olmedo F, Salcedo G, Aragoncillo C, Sanchez-Monge R, Collada C, Gomez L (1988) Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae. Electrophoresis 9:719–727
Gatehouse JA, Lycett GW, Delauney AJ, Croy BRD, Boulter D (1983) Sequence specificity of the post-translational proteolytic cleavage of vicilin, a seed storage protein of pea (Pisum sativum L.). Biochem J 212:427–432
Geburek Th, von Wuehlisch G (1989) Linkage analysis of isozyme gene loci in Picea abies (L.) Karst. Heredity 62:185–191
Gibbs PEM, Strongin KB, McPherson A (1989) Evolution of legume seed-storage proteins. A domain common to legumins and vicilins is duplicated in vicilins. Mol Biol Evol 6:614–623
Gifford DJ (1988) An electrophoretic analysis of the seed proteins from Pinus monticola and eight other species of pine. Can J Bot 66:1808–1812
Hager KP, Jensen U, Gilroy J, Richardson M (1992) The N-terminal amino acid sequence of the β-subunit of the legumin-like protein from seeds of Ginkgo biloba. Phytochemistry 31:523–525
Hakman I, Stabel P, Engstrom P, Eriksson T (1990) Storage protein accumulation during zygotic and somatic embryo development in Picea abies (Norway spruce). Physiol Plant 80:441–445
Higgins DG, Sharp PM (1988) CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73:237–244
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Leal I, Misra S (1993) Molecular cloning and characterization of a legumin-like storage protein cDNA of Douglas fir seeds. Plant Mol Biol 21:709–715
Lycett GW, Delauney AJ, Gatehouse JA, Gilroy J, Croy RD, Boulter D (1983) The vicilin gene family of pea (Pisum sativum L.): a complete cDNA coding sequence for preprovicilin. Nucleic Acids Res 11:2367–2380
Misra S, Green MJ (1990) Developmental gene expression in conifer embryogenesis and germination. I. Seed proteins and protein body composition of mature embryo and the megagametophyte of white spruce (Picea glauca [Moench] Voss). Plant Sci 68:163–173
Newton CH, Flinn BS, Sutton BCS (1992) Vicilin-like seed storage proteins in the gymnosperm interior spruce (Picea glauca/engelmanii) Plant Mol Biol 20:315–322
Perry DJ, Knowles P (1989) Inheritance and linkage relationships of allozymes of eastern white cedar (Thuja occidentalis) in north-western Ontario. Genome 32:245–250
Plietz P, Damaschum G, Müller JJ, Schelesier B (1983) Comparison of the structure of the 7S globulin from Phaseolus vulgaris in solution with the crystal structure of the 7S globulin from Canavalia ensiformis by small-angle X-ray scattering. FEBS Lett 162:43–46
Scholz G, Manteuffel R, Muntz K, Rudolph A (1983) Low-molecular-weight polypeptides of vicilin from Vicia faba L. are products of proteolytic breakdown. Eur J Biochem 132:103–107
Sebastiani FL, Farrell LB, Schuler MA, Beachy RN (1990) Complete sequence of a cDNA of α-subunit of soybean β-conglycinin. Plant Mol Biol 15:197–201
Shotwell MA, Larkins BA (1989) The biochemistry and molecular biology of seed storage proteins. In: Marcus A (ed) The biochemistry of plants. A comprehensive treatise, vol 15. Academic Press, London, pp 297–345
Slightom JL, Sun SM, Hall TC (1983) Complete nucleotide sequence of a French bean storage-protein gene: phaseolin. Proc Natl Acad Sci USA 80:1897–1901
Spencer D, Chandler PM, Higgins TJV, Inglis AS, Rubira M (1983) Sequence interrelationships of the subunits of vicilin from pea seeds. Plant Mol Biol 2:259–267
Tierney ML, Bray EA, Allen RD, Ma Y, Drong RF, Slightom J, Beachy RN (1987) Isolation and characterization of a genomic clone encoding the β-subunit of β-conglycinin. Planta 172:356–363
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Communicated by P. M. A. Tigerstedt
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Allona, I., Casado, R. & Aragoncillo, C. Biochemical genetics of a 7S globulin-like protein from Pinus pinaster seed. Theoret. Appl. Genetics 88, 454–459 (1994). https://doi.org/10.1007/BF00223660
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DOI: https://doi.org/10.1007/BF00223660