Summary
In the wandering spider Cupiennius salei, the functional neuroanatomy of leg mechanosensory receptor neurons and interneurons associated with a single leg neumere was investigated by combined intracellular recording and Lucifer yellow ionophoresis. Trichobothria axons that selectively respond to air currents and to low-frequency airborne vibrations have arborizations restricted to ventral regions of the appropriate leg neuromere. Receptor afferents that respond selectively to substrateborne vibrations are distributed ventrally in the corresponding leg neuromere and extend into certain interganglionic tract neuropiles. Golgi impregnation and intracellular dye filling show that local interneurons originate in ventral sensory neuropiles of leg neuromeres and ascend dorsally to terminate amongst dendrites of motor neurons. Local interneurons generally show higher thresholds for vibration stimuli than do receptors. Local interneurons typically receive inputs from one or several types of receptors. Some respond to stimulation of a single leg, others respond to stimulation of several legs on the same side of the body. The functional morphology of the receptor afferents is correlated with known physiological characteristics of slit sensilla and trichobothria. Structure and activity of the local interneurons are compared with analogous interneurons in other arthropods.
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References
Babu KS, Barth FG (1984) Neuroanatomy of the central nervous system of the wandering spider, Cupiennius salei (Arachnida, Araneida). Zoomorphology 104:344–359
Babu KS, Barth FG (1989) Central nervous projections of mechanoreceptors in the spider Cupiennius salei Keys. Cell Tissue Res (to appear)
Babu KS, Barth FG, Strausfeld NJ (1985) Intersegmental sensory tracts and contralateral motor neurons in the leg ganglia of the spider Cupiennius salei Keys. Cell Tissue Res 241:53–57
Bacon JP, Murphey RK (1984) Receptive fields of the cricket giant interneurons are related to their dendritic structure. J Physiol 352:601–623
Barth FG (1967) Ein einzelnes Spaltsinnesorgan auf dem Spinnentarsus: seine Erregung in Abhängigkeit von den Parametern des Luftschallreizes. Z Vergl Physiol 55:407–449
Barth FG (1982) Spiders and vibratory signals: sensory reception and behavioral significance. In: Witt P, Rovner JS (eds) Spider communication, mechanisms and ecological significance. Princeton University Press, Princeton, NY, pp 67–120
Barth FG (1985) Neuroethology of the spider vibration sense. In: Barth FG (ed) Neurobiology of arachnids. Springer, Berlin Heidelberg New York Tokyo, pp 203–229
Barth FG, Geethabali (1982) Spider vibration receptors: threshold curves of individual slits in the metatarsal lyriform organ. J Comp Physiol [A] 148:175–185
Bodian D (1936) A new method for staining nerve fibers and nerve endings in mounted paraffin sections. Anat Rec 69:89–97
Bräunig P, Hustert R, Pflüger HJ (1981) Distribution and specific central projections of mechanoreceptors in the thorax and proximal leg joints of locust. I. Morphology, location and innervation of internal proprioceptors of pro- and metathorax and their central projections. Cell Tissue Res 216:56–77
Burrows M, Pflüger H-J (1986) Processing by local interneurons of mechanosensory signals involved in a leg reflex of the locust. J Neurosci 6:2764–2777
Dahl F (1883) Über die Hörhaare bei den Arachnoiden. Zool Anz 6:267–270
Eckweiler W, Seyfarth E-A (1988) Tactile hairs and the adjustment of body height in wandering spiders: behavior, leg reflexes, and afferent projections in the leg ganglia. J Comp Physiol [A] 162:611–621
Eibl E, Huber F (1979) Central projections of tibial sensory fibres within the three thoracic ganglia of crickets (Gryllus campestris L., Gryllus bimaculatus de Geer). Zoomorphologie 92:1–17
Görner P, Andrews P (1969) Trichobothrien, ein Ferntastsinnesorgan bei Webespinnen (Araneen). Z Vergl Physiol 64:301–317
Gregory GE (1980) The Bodian protargol technique. In: Strausfeld NJ, Miller TA (eds) Neuroanatomical techniques. Springer, Berlin, Heidelberg New York, pp 75–95
Gronenberg W (1987) Neuronal elements in the CNS of a spider (Cupiennius salei): anatomy and physiology. In: Elsner N, Creutzfeld O (eds) New frontiers in brain research. Thieme, Stuttgart New York, p 78
Hanström B (1928) Vergleichende Anatomie des Nervensystems der wirbellosen Tiere. Springer, Berlin Heidelberg New York, pp 392–407
Hustert R (1978) Segmental and interganglionic projections from primary fibers of insect mechanoreceptors. Cell Tissue Res 194:337–351
Hustert R, Pflüger HJ, Bräunig P (1981) Distribution and specific central projections of mechanoreceptors in the thorax and proximal leg joints of locusts. III. The external mechanoreceptors: the campaniform sensilla. Cell Tissue Res 216:97–111
Kondoh Y, Hisada M (1986) Distribution and ultrastructure of synapses on a premotor local nonspiking interneuron of the crayfish. J Comp Neurol 254:259–270
Liesenfeld FJ (1961) Über Leistung und Sitz des Erschütterungssinnes von Netzspinnen. Biol Zentralbl 80:465–475
Linsenmair KE (1968) Anemomenotaktische Orientierung bei Skorpionen (Chelicerata, Scorpiones). Z Vergl Physiol 60:445–449
Markl H (1973) Leistungen des Vibrationssinnes bei wirbellosen Tieren. Fortschr Zool 21:100–120
Markl H, Tautz J (1975) The sensitivity of hair receptors in caterpillars of Barathra brassicae L. (Lepidoptera, Noctuidae) to particle movement in a sound field. J Comp Physiol [A] 99:79–87
Milde J, Seyfarth E-A (1988) Tactile hairs and leg reflexes in wandering spiders: physiological and anatomical correlates of reflex activity in the leg ganglia. J Comp Physiol [A] 162:623–633
Palka J, Levine R, Schubiger M (1977) The cercus-to-giant interneuron system of crickets. I. Some attributes of the sensory cells. J Comp Physiol [A] 119:267–283
Pflüger H-J, Tautz J (1982) Air movements sensitive hairs and interneurons in Locusta migratoria. J Comp Physiol [A] 145:369–380
Pflüger HJ, Bräunig P, Hustert R (1981) Distribution and specific central projections of mechanoreceptors in the thorax and proximal leg joints of locusts. II. The external mechanoreceptors: hair plates and tactile hairs. Cell Tissue Res 216:79–96
Reichert H, Plummer MR, Wine JJ (1983) Identified nonspiking local interneurons mediate nonrecurrent, lateral inhibition of crayfish mechanosensory interneurons. J Comp Physiol [A] 151:261–276
Reissland A (1978) Electrophysiology of trichobothria in orbweaving spiders (Agelenidae, Araneae). J Comp Physiol [A] 123:71–84
Reissland A, Görner P (1978) Mechanics of trichobothria in orbweaving spiders (Agelenidae, Araneae). J Comp Physiol [A] 123:59–69
Reissland A, Görner P (1985) Trichobothria. In: Barth FG (ed) Neurobiology of arachnids. Springer, Berlin Heidelberg New York Tokyo, pp 138–161
Ribi WA (1983) Electron microscopy of Golgi-impregnated neurons. In: Strausfeld NJ (ed) Functional neuroanatomy. Springer, Berlin Heidelberg New York Tokyo, pp 1–18
Römer H, Marquart V (1984) Morphology and physiology of auditory interneurons in the metathoracic ganglion of the locust. J Comp Physiol [A] 155:249–262
Shimozawa T, Kanou M (1984) Varieties of filiform hairs: range fractionation by sensory afferents and cereal interneurons of a cricket. J Comp Physiol [A] 155:485–493
Siegler MVS, Burrows M (1983) Spiking local interneurons as primary integrators of mechanosensory information in the locust. J Neurophysiol 50:1281–1295
Siegler MVS, Burrows M (1984) The morphology of two groups of spiking local interneurons in the metathoracic ganglion of the locust. J Comp Neurol 224:463–482
Speck J, Barth FG (1982) Vibration sensitivity of pretarsal slit sensilla in the spider leg. J Comp Physiol [A] 148:187–194
Speck-Hergenröder J, Barth FG (1987) Tuning of vibration sensitive neurons in the central nervous system of a wandering spider, Cupiennius salei Keys. J Comp Physiol [A] 160:467–475
Spurr AR (1969) A low viscosity epoxyresin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Tobias M, Murphey RK (1979) The response of cereal receptors and identified interneurons in the cricket (Acheta domesticus) to air currents. J Comp Physiol [A] 129:51–59
Tyrer NM, Bacon JP, Davies CA (1979) Sensory projections from the wind-sensitive head hairs of the locust Schistocerca gregaria. I. Distribution in the central nervous system. Cell Tissue Res 203:79–92
Watson AHD, Burrows M (1982) The ultrastructure of identified motor neurones and their synaptic relationships. J Comp Neurol 205:383–347
Watson AHD, Burrows M (1983) The morphology, ultrastructure, and distribution of synapses on an intersegmental interneuron of the locust. J Comp Neurol 214:154–169
Watson AHD, Burrows M (1985) The distribution of synapses on the two fields of neurites of spiking local interneurons in the locust. J Comp Neurol 240:219–232
Wohlers DW, Huber F (1978) Intracellular recording and staining of cricket auditory interneurons (Gryllus campestris L., Gryllus bimaculatus de Geer). J Comp Physiol [A] 127:11–28
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Gronenberg, W. Anatomical and physiological observations on the organization of mechanoreceptors and local interneurons in the central nervous system of the wandering spider Cupiennius salei . Cell Tissue Res. 258, 163–175 (1989). https://doi.org/10.1007/BF00223155
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DOI: https://doi.org/10.1007/BF00223155