Abstract
Germplasm of Triticum dicoccoides collected from different environments in Israel was evaluated by using the PCR as a molecular marker. Two pairs of primers were used in the PCR in amplifying the DNA tracts coding the high-and low-molecular-weight glutenin subunits. Analyses reveal great variability within and between populations indicating the high values of this germplasm for future breeding programs to improve the protein quality in wheat.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beckmann JS, Soller M (1986) Restriction fragment length polymorphism in plant genetic improvement. Oxford Surveys of Plant Mol Cell Biol 3:196–250
Beckmann JS, Soller M (1988) Detection of linkage between marker loci and loci affecting quantitative traits in crosses between segregating populations. Theor Appl Genet 76:228–236
Brown AHD (1989) The case for core collections. In: Brown AHD et al. (eds) The use of plant genetic resources. Cambridge University Press, Cambridge, pp 136–156
Carver BF, Nevo E (1990) Genetic diversity of photosynthetic characters in native populations of Triticum dicoccoides. Photosyn Res 25:119–128
Colot V, Bartels D, Thompson R, Flavell R (1989) Molecular characterisation of an active wheat LMW glutenin gene and its relation to the other wheat and barley prolamin genes. Mol Gen Genet 216:81–90
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini preparation: version II. Plant Mol Biol Rep 1:19–21
D'Ovidio R, Tanzarella OA, Porceddu E (1990) Rapid and efficient detection of genetic polymorphism in wheat through amplification by the polymerase chain reaction. Plant Mol Biol 15:169–171
D'Ovidio R, Tanzarella OA, Porceddu E (1992) Molecular analysis of gliadin and glutenin genes in T. durum cv Lira. A model system to analyse the molecular bases of quality differences in durum wheat cultivars. J Cereal Sci 16:165–172
D'Ovidio R, Anderson OD, Porceddu E (1993) PCR identification of specific gliadin and glutenin genes in wheat. Vth Gluten Workshop. Detmald, Germany, June 7–9,. In: Gluten proteins, published by Association of cereal research, Detmold, Germany, pp. 326–334
Feldman M, Sears ER (1981) The wild gene resources of wheat. Sci Am 244:102–112
Flavell RB, O'Dell M, Sharp PJ, Nevo E, Beiles A (1986) Variation in the intergenic spacer of ribosomal DNA of wild wheat, Triticum dicoccoides in Israel. Mol Biol Evol 3:547–558
Frankel OH (1984) Genetic prospectives of germplasm conservation. In: Arber WK et al. (eds) Genetic manipulation: impact on man and society. Cambridge University Press, Cambridge, pp 161–170
Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR (1987) The nucelotide and deduced amino-acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor Appl Genet 75:117–126
Helentjaris T, King G, Slocum M, Siedenstrang C, Wegman S (1985) Restriction fragment length polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol Biol 5:109–118
Innis MA, Myambo KB, Gelfand DH, Brown MAD (1988) DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proc Natl Acad Sci USA 85:9436–9440
Mullis KB, Faloona F (1987) Specific synthesis of DNA in vitro via a polymerase-catalysed chain reaction. In: Methods Enzymol 155:335–350
Nei M (1973) Analysis of genetic diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590
Nevo E (1983) Genetic resources of wild emmer wheat: structure, evolution and application in breeding. In: Sakamoto S (ed) Proc 6th Int Wheat Genet Symp. Kyoto, Japan, pp 421–431
Nevo E (1986) Genetic resources of wild cereals and crop improvement: Israel a natural laboratory. Isr J Bot 35:255–278
Nevo E (1987) Plant genetic resources: prediction by isozyme markers and ecology. In: Rattazzi MC, Scandalios JG, Whitt GS (eds) Isozymes: current topics in biological and medical research, vol 16. Alan R. Liss, New York, pp 247–267
Nevo E (1988) Genetic diversity in nature: patterns and theory. Evol Biol 23:217–247
Nevo E (1993) Genetic resources of wild emmer, Triticum dicoccoides, for wheat improvement: news and views (abstract). 8th Int Wheat Genet Symp, Beijing p 3 orNevo E (1995) Origin, evolution, population genetics and resources for breeding of wild emmer wheat, Triticum dicoccoides, in the Fertile Crescent. In: Kaloo G (ed) Wild taxa of crop plants: potential plant genetic resources (in press)
Nevo E, Beiles A (1989) Genetic diversity of wild emmer wheat in Israel and Turkey: structure, evolution and application in breeding. Theor Appl Genet 77:421–455
Nevo E, Payne PI (1987) Wheat storage proteins: diversity of HMW subunits in wild emmer from Israel. I. Geographical patterns and ecological predictability. Theor Appl Genet 74:827–836
Nevo, E, Golenberg EM, Beiles A, Brown AHD, Zohary D (1982) Genetic diversity and environmental associations of wild wheat, Triticum dicoccoides, in Israel. Theor Appl Genet 62:241–254
Nevo E, Beiles A, Krugman T (1988a) Natural selection of allozyme polymorphisms: a microgeographical differentiation by edaphic, topographical, and temproal factors in wild emmer wheat (Triticum dicoccoides). Theor Appl Genet 76:737–752
Nevo E, Beiles A, Krugman T (1988b) Natural selection of allozyme polymorphisms: a microgeographical climatic differentiation in wild emmer wheat (Triticum dicoccoides). Theor Appl Genet 75:529–538
Nevo E, Gerechter-Amitai Z, Beiles A (1991) Resistance of wild emmer wheat to stem rust: ecological, pathological and alloyzme associations. Euphytica 53:121–130
Nevo E, Pagnotta MA, Beiles A, Porceddu E (1995) Wheat storage proteins: glutenin DNA diversity in wild emmer wheat, Triticum dicoccoides, in Israel and Turkey. 3. Environmental correlates and allozymic associations. Theor Appl Genet 91:415–420
Plucknett DL, Smith NJH, Williams JT, Anishetty NM (1983) Crop germplasm conservation and developing countries. Science 220:163–169
Plucknett DL, Smith NJH, Williams JT, Anishetty NM (1987) Gene banks and the world's food. Princeton University Press, Princeton, New Jersey
Saiki RK, Scarf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of beta-globin genomic sequences and restriction-site analysis for diagnosis of sickle-cell anaemia. Science 230:1350–1354
Schwarz K, Hansen-Hagge T, Bartram C (1990). Improved yields of long PCR products using gene 32 protein. Nucleic Acids Res 18:1079
SPSS (1990) User's guide, Release 4. SPSS Inc. Chicago
Swofford DL, Selander RB (1989) Biosys-1, Release 1.7. Published by the first author, Champaign, Illinois, USA
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Biotechnology 7:257–264
Wright S (1943) Isolation by distance. Genetics 28:114–138
Zohary D (1970) Wild wheat. In: Frankel OH, Bennet E (eds) Genetic resources in plants: their exploration and conservation. Blackwell, Oxford Edinburgh, pp 239–247
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Pagnotta, M.A., Nevo, E., Beiles, A. et al. Wheat storage proteins: glutenin diversity in wild emmer, Triticum dicoccoides, in Israel and Turkey. 2. DNA diversity detected by PCR. Theoret. Appl. Genetics 91, 409–414 (1995). https://doi.org/10.1007/BF00222967
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222967