Skip to main content
Log in

Appearance and innervation of CHH-producing cells in the eyestalk of the crayfish Astacus leptodactylus examined after tracing with Lucifer Yellow

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

By injection of the fluorescent dye Lucifer Yellow into individual Crustacean Hyperglycemic Hormone (CHH)-producing cells, the shape of these neurosecretory cells in the eyestalk of the crayfish Astacus leptodactylus can be traced. A highly fluorescent perikaryon gives rise to an axon that can be followed by the fluorescent label to the neurohemal region, the sinus gland. The proximal part of that axon sends out extensive branches into the neuropil of the medulla terminalis. Electron-microscopic investigations reveal synaptic input to these axonal ramifications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adams ME, O'Shea M (1983) Peptide cotransmitter at a neuromuscular junction. Science 221:286–289

    Google Scholar 

  • Andrew RD, Saleuddin ASM (1978) Structure and innervation of a crustacean neurosecretory cell. Can J Zool 56:423–430

    Google Scholar 

  • Andrew RD, Orchard I, Saleuddin ASM (1978) Structural re-evaluation of the neurosecretory system in the crayfish eyestalk. Cell Tissue Res 190:235–246

    Google Scholar 

  • Baskin DG, Erlandsen SL, Parsons JA (1979) Immunocytochemistry with osmium-fixed tissue. I. Light microscopic localizationof growth hormone and prolactin with the unlabeled antibody-enzyme method. J Histochem Cytochem 27:867–872

    Google Scholar 

  • Buijs RM, Swaab DF (1979) Immuno-electron microscopical demonstration of vasopressin and oxytocin synapses in the limbic system of the rat. Cell Tissue Res 204:355–365

    Google Scholar 

  • Drach P (1944) Etude préliminaire sur le cycle d'intermue et son conditionnement hormonal chez Leander serratus (Pennant). Bull Biol Fr Belg 78:40–62

    Google Scholar 

  • Emson PC (1979) Peptides as neurotransmitter candidates in the mammalian CNS. Prog Neurobiol 13:61

    Google Scholar 

  • Glantz RM, Kirk MD, Arechiga H (1983) Light input to crustacean neurosecretory cells. Brain Res 265:307–311

    Google Scholar 

  • Gorgels-Kallen JL (1984) Etude histophysiologique du système neurosécrétoire produisant l'hormone hyperglycémique dans le pédoncule oculaire de l'écrevisse. VIII Réunion des Carcinologistes de Langue Française (Liège, 29.08–02.09.1983). Ann Soc R Zool Belgique 114:171–172 (abstract)

    Google Scholar 

  • Gorgels-Kallen JL, Meij JTA (1984) Description of the crustacean hyperglycemic hormone (CHH)-producing system in the eyestalk of the crayfish Astacus leptodactylus during larval and postlarval development, determined by means of immunocytochemistry. J Morphol (in press)

  • Gorgels-Kallen JL, Van Herp F (1981) Localization of crustacean hyperglycemic hormone (CHH) in the X-organ sinus gland complex in the eyestalk of the crayfish, Astacus leptodactylus (Nordmann, 1842). J Morphol 170:347–355

    Google Scholar 

  • Gorgels-Kallen JL, Van Herp F (1983) Does molecular heterogeneity exist for the crustacean hyperglycemic hormone produced in the neurosecretory system of the crayfish Astacus leptodactylus? Biosynthèse, métabolisme, mode d'action des hormones d'invertébrés. International CNRS Symposium, Strasbourg, p 116 (abstract)

  • Gorgels-Kallen JL, Voorter CEM (1984) Secretory stages of individual CHH-producing cells in the eyestalk of the crayfish Astacus leptodactylus, determined by means of immunocytochemistry. Cell Tissue Res 237:291–298

    Google Scholar 

  • Gorgels-Kallen JL, Van Herp F, Leuven RSEW (1982) A comparative immunocytochemical investigation of the crustacean hyperglycemic hormone (CHH) in the eyestalks of some decapod Crustacea. J Morphol 174:161–168

    Google Scholar 

  • Jacobs AAC, Van Herp F (1984) Immunocytochemical localization of a substance in the eyestalk of the prawn Palaemon serratus, reactive with an anti-FMRF amide rabbit serum. Cell Tissue Res 235:601–605

    Google Scholar 

  • Jaros PP (1978) Tracing of neurosecretory neurons in crayfish optic ganglia by cobalt iontophoresis. Cell Tissue Res 194:297–302

    Google Scholar 

  • Jaros PP, Keller R (1979) Immunocytochemical identification of hyperglycemic hormone-producing cells in the eyestalk of Carcinus maenas. Cell Tissue Res 204:379–385

    Google Scholar 

  • Keller R (1981) Purification and amino acid composition of the hyperglycemic neurohormone from the sinus gland of Orconectes limosus and comparison with the hormone of Carcinus maenas. J Comp Physiol 141:445–450

    Google Scholar 

  • Keller R, Beyer J (1968) Zur hyperglykämischen Wirkung von Serotonin und Augenstielextrakt beim Flußkrebs Orconectes limosus. Z vergl Physiol 59:78–85

    Google Scholar 

  • Kleinholz LH, Keller R (1979) Endocrine regulation in crustacea. In: Barrington EJW (ed) Hormones and evolution. Academic Press, New York, vol 1, pp 160–213

    Google Scholar 

  • Lane BP, Europe DL (1965) Differential staining of ultrathin sections of Epon-embedded tissues for light microscopy. J Histochem Cytochem 13:579–582

    CAS  PubMed  Google Scholar 

  • Mancillas JR, McGinty JF, Selverston AJ, Karten H, Bloom EE (1981) Immunocytochemical localization of enkephalin and substance P in retina and eyestalks neurones of lobster. Nature 293:576–578

    Google Scholar 

  • Martin G (1978) Action de la sérotonine sur la glycémie et sur la libération des neurosécrétions contenues dans la glande du sinus de Porcellio dilatatus Brandt (Crustacé, Isopode, Oniscoïde). CR Soc Biol 172:304–308

    Google Scholar 

  • Martin G, Dubois MP (1981) A somatostatin-like antigen in the nervous system of an isopod Porcellio dilatatus Brandt. Gen Comp Endocrinol 45:125–130

    Google Scholar 

  • Newcomb RW (1983) Peptides in the sinus gland of Cardisoma carnifex: isolation and amino acid analysis. J Comp Physiol B 153:207–221

    Google Scholar 

  • Polak JM, Bloom SR (1978) Peptidergic nerves of the gastrointestinal tract. Invest Cell Pathol 1:301–326

    Google Scholar 

  • Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212

    Article  CAS  PubMed  Google Scholar 

  • Sedlmeier D, Keller R (1981) The mode of action of the crustacean neurosecretory hyperglycemic hormone. I. Involvement of cyclic nucleotides. Gen Comp Endocrinol 45:82–90

    Google Scholar 

  • Shivers RR (1967) Fine structure of crayfish optic ganglia. Univ Kansas Sci Bull XLVII:677–733

    Google Scholar 

  • Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43

    CAS  PubMed  Google Scholar 

  • Steel CGH (1977) The neurosecretory system in the aphid Megoura viciae, with reference to unusual features associated with long distance transport of neurosecretion. Gen Comp Endocrinol 31:307–322

    Google Scholar 

  • Sternberger LA (1974) Immunocytochemistry. Prentice-Hall Inc., Englewood Cliffs, New Jersey, 246 pp

    Google Scholar 

  • Stewart WW (1978) Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell 14:741–759

    Google Scholar 

  • Strolenberg GECM (1979) Functional aspects of the sinus gland in the neurosecretory system of the crayfish Astacus leptodactylus: An ultrastructural approach. Catholic University Nijmegen, The Netherlands, Thesis, pp 140

    Google Scholar 

  • Strolenberg GECM, Van Herp F (1977) Mise en évidence du phénomène d'exocytose dans la glande du sinus d'Astacus leptodactylus (Nordmann) sous l'influence d'injections de sérotonine. CR Acad Sci D 284:57–60

    Google Scholar 

  • Van Deijnen JE, Vek F, Van Herp F (1984) Une étude immunocytochimique des pédoncules oculaires de l'écrevisse Astacus leptodactylus: réactions immunopositives avec différents anticorps contre des neuropeptides et peptides gastro-intestinaux de vertébrés VIII Réunion des Carcinologistes de Langue Française (Liège, 29.08–02.09.1983) Ann Soc R Zool Belgique 114:178 (abstract)

    Google Scholar 

  • Van Herp F, Bellon-Humbert C (1982) Localisation immunocytocheimique de substances apparentées à la neurophysine et à la vasopressine dans le pédoncule oculaire de Palaemon serratus Pennant (Crustacé Décapode Natantia). CR Acad Sci Paris 295:97–102

    Google Scholar 

  • Van Herp F Van Buggenum HJM (1979) Immunocytochemical localization of hyperglycemic hormone (HGH) in the neurosecretory system of the eyestalk of the crayfish Astacus leptodactylus. Experientia 35:1527–1528

    Google Scholar 

  • Van Herp F, Strolenberg GECM (1980) Functional aspects of the neurosecretory system in the eyestalk of the crayfish Astacus leptodactylus with special reference to the hyperglycemic hormone. Gen Comp Endocrinol 40:364 (abstract)

    Google Scholar 

  • Van Herp F, Van Wormhoudt A, Van Venrooy WAJ, Bellon-Humbert C (1984) Immunocytochemical study of Crustacean Hyperglycemic Hormone (CHH) in the eyestalks of the prawn Palaemon serratus (Pennant) and some other Palaemonidae, in relation to variations in the blood glucose level. J Morphol 182:85–94

    Google Scholar 

  • Van Wormhoudt A, Van Herp F, Bellon-Humbert C, Keller R (1984) Polymorphisme de l'hormone hyperglycémique chez Palaemon serratus (Crustacea, Decapoda, Natantia). VIII Réunion des Carcinologistes de Langue Française (Liège, 29.08–02.09.1983) Ann Soc R Zool Belgique 114:179–180 (abstract)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gorgels-Kallen, J.L. Appearance and innervation of CHH-producing cells in the eyestalk of the crayfish Astacus leptodactylus examined after tracing with Lucifer Yellow. Cell Tissue Res. 240, 385–391 (1985). https://doi.org/10.1007/BF00222350

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00222350

Key words

Navigation